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In vivo blending of medium chain length polyhydroxy-alkanoates and polyhydroxybutyrate using recombinant Pseudomonas putida harboring phbCAB operon of Ralstonia eutropha

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Abstract

The in vivo blending of medium chain length polyhydroxyalkanoates (mcl-PHA) and polyhydroxybutyrate (PHB) was carried out using recombinant Pseudomonas putida after transforming the phbCAB operon of Ralstonia eutropha. The most suitable carbon sources for the production of mcl-PHA and PHB blends were identified to be octanoate and gluconate. The molar fractions of 3-hydroxyoctanoate and 3-hydroxybutyrate in the polymer blends were effectively modulated by controlling the mixing ratio of octanoate and gluconate, thereby producing a composition ranging from 95% mcl-PHA to 78% PHB.

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Authors and Affiliations

  1. Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University, Daegu, 702-701, Korea

    Hyun-Dong Shin, Jin-Nam Lee & Yong-Hyun Lee

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  1. Hyun-Dong Shin
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  2. Jin-Nam Lee
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  3. Yong-Hyun Lee
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Shin, HD., Lee, JN. & Lee, YH. In vivo blending of medium chain length polyhydroxy-alkanoates and polyhydroxybutyrate using recombinant Pseudomonas putida harboring phbCAB operon of Ralstonia eutropha . Biotechnology Letters 24, 1729–1735 (2002). https://doi.org/10.1023/A:1020604915657

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