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The Effect of γ-Radiation and Desiccation on the Viability of the Soil Bacteria Isolated from the Alienated Zone around the Chernobyl Nuclear Power Plant

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Abstract

Methylobacterium extorquens, M. mesophilicum, and Bacillus subtilis strains were found to be resistant to γ-radiation, irrespective of whether they were isolated from the alienated zone around the Chernobyl Nuclear Power Plant or outside this zone. The LD90 of Methylobacterium and B. subtilis strains with respect to γ-radiation was 2.0–3.4 and 3.7–4.4 kGy, respectively, whereas their LD99.99 values were 4.5–6.9 and more than 10 kGy, respectively. The high threshold levels of γ-radiation for Methylobacterium and B. subtilis imply the efficient functioning of DNA repair systems in these bacteria. Unlike Bacillus polymyxa cells, the cells of M. extorquens, M. mesophilicum, and B. subtilis were also resistant to desiccation. Pseudomonas sp., Nocardiasp., and nocardioform actinomycetes were sensitive to both γ-radiation and desiccation. Similar results were obtained when the bacteria studied were exposed to hydrogen peroxide and ultraviolet radiation. The results obtained indicate that the bacteria that are resistant to γ-radiation are also resistant to desiccation, UV radiation, and hydrogen peroxide. The possibility of using common laboratory tests (such as the determination of bacterial resistance to UV light and desiccation) for the evaluation of bacterial resistance to γ-radiation is discussed.

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REFERENCES

  1. Romanovskaya, V.A., Rokitko, P.V., Malashenko, Yu.R., and Chernaya, N.A., Sensitivity of Soil Bacteria Isolated from the Alienated Zone around the Chernobyl Nuclear Power Plant to Various Stress Factors, Mikrobiologiya, 1999, vol. 68, no. 4, pp. 534–539.

    Google Scholar 

  2. Romanovskaya, V.A., Malashenko, Yu.R., Sokolov, I.G., and Rokitko, P.V., Mutability of Epiphytic and Soil Bacteria of the Genus Methylobacterium and Their Resistance to Ultraviolet and Ionizing Radiation, Mikrobiologiya, 1998, vol. 67, no. 1, pp. 106–115.

    Google Scholar 

  3. Sanders, S.W. and Maxcy, R.B., Isolation of Radiation-Resistant Bacteria without Exposure to Radiation, Appl. Environ. Microbiol., 1979, vol. 38, no. 3, pp. 436–439.

    Google Scholar 

  4. Mattimore, V. and Battista, J.R., Radioresistance of Deinococcus radiodurans: Functions Necessary to Survive Ionizing Radiation Are Also Necessary to Survive Prolonged Desiccation, J. Bacteriol., 1996, vol. 178, no. 3, pp. 633–637.

    Google Scholar 

  5. Asada, S., Takano, M., and Shibasaki, I., Deoxyribonucleic Acid Strand Breaks during Drying of Escherichia coli on a Hydrophobic Filter Membrane, Appl. Environ. Microbiol., 1979, vol. 37, no. 2, pp. 266–273.

    Google Scholar 

  6. Asada, S., Takano, M., and Shibasaki, I., Mutation Induced by Drying of Escherichia coli on a Hydrophobic Filter Membrane, Appl. Environ. Microbiol., 1980, vol. 40, no. 2, pp. 274–281.

    Google Scholar 

  7. Dose, K. and Gill, M., DNA Stability and Survival of Bacillus subtilis Spores in Extreme Dryness, Orig. Life Evol. Biosph., 1995, vol. 25, nos. 1-3, pp. 277–293.

    Google Scholar 

  8. Dose, K. and Klein, A., Response of Bacillus subtilis Spores to Dehydration and UV Irradiation at Extremely Low Temperatures, Orig. Life Evol. Biosph., 1996, vol. 26, no. 1, pp. 47–59.

    Google Scholar 

  9. Romanovskaya, V.A., Stolyar, S.M., and Malashenko, Yu.R., Distribution of Bacteria from the Genus Methylobacterium in Various Ecosystems of Ukraine, Mikrobiol. Zh. (Kiev), 1996, vol. 58, no. 3, pp. 3–9.

    Google Scholar 

  10. Romanovskaya, V.A., Sokolov, I.G., Rokitko, P.V., and Chernaya, N.A., The Effect of Radioactive Contamination on Soil Bacteria in the 10-km Zone around the Chernobyl Nuclear Power Plant, Mikrobiologiya, 1998, vol. 67, no. 2, pp. 274–280.

    Google Scholar 

  11. Yarmonenko, S.P., Radiobiologiya cheloveka i zhivotnykh (Human and Animal Radiobiology), Moscow: Vysshaya Shkola, 1984, p. 67.

    Google Scholar 

  12. Nazim, A. and James, A., Life under Intense Irradiation, Microbial Life in Extreme Environments, Kushner, D.J., Ed., London: Academic, 1978. Translated under the title Zhizn' mikrobov v ekstremal'nykh usloviyakh, Moscow: Mir, 1981, pp. 472-504.

    Google Scholar 

  13. Moseley, B.E.B., The Isolation and Some Properties of Radiation-Sensitive Mutants of Micrococcus radiodurans, J. Gen. Microbiol., 1967, vol. 49, no. 2, pp. 203–300.

    Google Scholar 

  14. Ito, H. and Iizuca, H., Characterization of Radiation-Resistant Species of Pseudomonas radiora and Pattern of Catalase Activity, Agric. Biol. Chem., 1980, vol. 44, no. 6, pp. 1315–1320.

    Google Scholar 

  15. Potts, M., Desiccation Tolerance of Prokaryotes, Microbiol. Rev., 1994, vol. 58, no. 4, pp. 775–805.

    Google Scholar 

  16. Daly, M.J. and Minton, K.W., Resistance to Radiation, Science, 1995, vol. 270, no. 24, p. 1318.

    Google Scholar 

  17. Philips, R.W., Berry, C.J., Fliermans, C., Wiegel, J., and Shimkets, L.J., Radiation and Desiccation Resistance in a Kineococcus-Like Organism, Third Int. Congress on Extremophiles, Hamburg, Germany, September 3-7, 2000, poster 96.

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Authors and Affiliations

  1. Zabolotnyi Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, ul. Zabolotnogo 154, Kiev, 252627, Ukraine

    V. A. Romanovskaya, P. V. Rokitko, Yu. R. Malashenko & N. A. Chernaya

  2. Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, Ukraine

    A. N. Mikheev & N. I. Gushcha

Authors
  1. V. A. Romanovskaya
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  2. P. V. Rokitko
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  3. A. N. Mikheev
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  4. N. I. Gushcha
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  5. Yu. R. Malashenko
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  6. N. A. Chernaya
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Romanovskaya, V.A., Rokitko, P.V., Mikheev, A.N. et al. The Effect of γ-Radiation and Desiccation on the Viability of the Soil Bacteria Isolated from the Alienated Zone around the Chernobyl Nuclear Power Plant. Microbiology 71, 608–613 (2002). https://doi.org/10.1023/A:1020575223365

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