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New Criteria for the Evaluation of Soil Bacterial Complexes

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Abstract

The initial concentration of prokaryotic microorganisms, the type of their growth, doubling time, and the growth dynamics of bacteria and actinomycetes in three types of soil (meadow, chestnut, and soddy forest) were evaluated by the luminescence microscopic analysis of soil samples incubated in a humid chamber for 1 day. Soddy forest and chestnut soils differed in most of the parameters analyzed. Meadow soil was close to soddy forest soil in some parameters and to chestnut soil in other parameters. All soil suspensions exhibited high growth rates of bacteria and actinomycetes, indicating that the fraction of viable microorganisms in the soils was high.

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REFERENCES

  1. Meisel', M.N. and Zavarzina, N.B. Investigation of Living Microbial Cells by Fluorescence Microscopy, Mikrobiologiya, 1947, vol. 16, no. 5, pp. 394–402.

    Google Scholar 

  2. Razumovskaya, Z.G. and Osipova, I.V., On the Ratio between Dead and Live Cells in a Growing Acetobacter melanogenum Culture, Mikrobiologiya, 1958, vol. 27, no. 6, pp. 727–731.

    Google Scholar 

  3. Babiuk, L.A. and Paul, E.A., The Use of Fluorescein Isothiocyanate in the Determination of the Bacterial Biomass of Grassland Soil, Can. J. Microbiol., 1970, vol. 16, pp. 57–62.

    Google Scholar 

  4. Coleman, A., Enhanced Detection of Bacteria in Natural Environments by Fluorochrome Staining of DNA, Limnol. Oceanogr., 1980, vol. 25, pp. 948–951.

    Google Scholar 

  5. Lusta, K.A. and Fikhte, B.A., Metody opredeleniya zhiznesposobnosti mikroorganizmov (Methods for Determining the Viability of Microorganisms), Eroshin, V.K., Ed., Pushchino: Nauchn. Tsentr Biol. Issl. Akad. Nauk SSSR, 1990.

    Google Scholar 

  6. Chrzanowski, T.H., Crotty, R.D., Hubbard, J.G., and Welch, R.P., Applicability of the Fluorescein Diacetate Method of Detecting Active Bacteria in Freshwater, Microb. Ecol., 1984, vol. 10, pp. 179–185.

    Google Scholar 

  7. Fry, J.C., Direct Methods of Biomass Estimation, Methods in Microbiology, Grigorova, R. and Norris, Y.K., Eds., London: Academic, 1990, vol. 22, pp. 41–81.

    Google Scholar 

  8. Lundgren, B., Fluorescein Diacetate as a Stain of Metabolically Active Bacteria in Soil, Oikos, 1981, vol. 36, pp. 17–22.

    Google Scholar 

  9. Polyanskaya, L.M., Golovchenko, A.V., and Zvyagintsev, D.G., Determination of the Viability of Fungal Spores and Mycelium in Soil, Mikrobiologiya, 1998, vol. 67, pp. 832–836.

    Google Scholar 

  10. Kjoller, A. and Struwe, S., Microfungi in Ecosystems: Fungal Occurrence and Activity in Litter and Soil, Oikos, 1982, vol. 39, pp. 389–422.

    Google Scholar 

  11. Metody pochvennoi mikrobiologii i biokhimii (Methods of Soil Microbiology and Biochemistry), Zvyagintsev, D.G., Ed., Moscow: Mosk. Gos. Univ., 1991.

    Google Scholar 

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Authors and Affiliations

  1. Department of Soil Biology, Faculty of Soil Science, Moscow State University, Vorob'evy gory, Moscow, 119899, Russia

    A. M. Polyanskii, L. M. Polyanskaya & D. G. Zvyagintsev

  2. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Leninskii pr. 33, Moscow, 117071, Russia

    A. V. Golovchenko

  3. Institute of Soil Science of the Moscow State University and Russian Academy of Sciences, Moscow, Russia

    A. V. Golovchenko

Authors
  1. A. M. Polyanskii
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  2. A. V. Golovchenko
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  3. L. M. Polyanskaya
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  4. D. G. Zvyagintsev
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Polyanskii, A.M., Golovchenko, A.V., Polyanskaya, L.M. et al. New Criteria for the Evaluation of Soil Bacterial Complexes. Microbiology 71, 582–586 (2002). https://doi.org/10.1023/A:1020566921547

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  • DOI: https://doi.org/10.1023/A:1020566921547

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