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Efficient production of doubled haploid plants by immediate colchicine treatment of isolated microspores in winter Brassica napus

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Abstract

The effect of colchicine on embryogenesis induction and chromosomedoubling during microspore culture was evaluated in two F1 hybridsofwinter oilseed rape (Brassica napus L.). Colchicinetreatment (50 and 500 mg/L) of isolated microspores during thefirst 15 h in culture stimulated embryogenesis and produced large amounts ofhealthy-looking embryos. These normal embryos germinated well at 24°C after being transferred to solid regeneration medium and aninitial period of low temperature (2 °C) for 10 days, andcoulddirectly and rapidly regenerate vigorous plants. A high doubling efficiency of84–88% was obtained from 500 mg/L colchicine treatment for15h with low frequency of polyploid and chimeric plants. Acolchicinetreatment duration of 6 h was less effective on embryogenesis anddoubling efficiency. The present experiment also showed that changing of induction medium 15h after microspore isolation produced higher spontaneous doublingefficiency, as compared with medium change 6 h after isolation.

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Authors and Affiliations

  1. College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310029, China

    W.J. Zhou & G.X. Tang

  2. Nilsson-Ehle Laboratory, Svalof Weibull AB, 26881, Svalov, Sweden

    P. Hagberg

Authors
  1. W.J. Zhou
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  2. G.X. Tang
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  3. P. Hagberg
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Zhou, W., Tang, G. & Hagberg, P. Efficient production of doubled haploid plants by immediate colchicine treatment of isolated microspores in winter Brassica napus . Plant Growth Regulation 37, 185–192 (2002). https://doi.org/10.1023/A:1020561201125

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  • DOI: https://doi.org/10.1023/A:1020561201125

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