Abstract
The absolute absorption spectra of the two heme a moieties in cytochrome c oxidase were determined in the Soret region where spectral contributions from copper ions are negligible. This determination employs a set of absorption spectra of the enzyme recorded during anaerobic reduction with sodium dithionite, and does not require any other spectral data. The unique feature of the component spectra revealed in the present study suggests the existence of a specific interaction of heme a with the immediate environment as its origin. The usefulness of the absolute spectra in quantitative analysis of the spectral data is presented.
Similar content being viewed by others
REFERENCES
Babcock, G. T., and Wikström, M. (1992). Nature 356, 301-309.
Beratan, D. N., Onuchic, J. N., Winkler, J. R., and Gray, H. B. (1992). Science 258, 1740-1741.
Bickar, D., Turrens, J. F., and Lehninger, A. L. (1986). J. Biol. Chem. 261, 14461-14466.
Bombelka, E., Richter, F.-W., Stroh, A., and Kadenback, B. (1986). Biochem. Biophys. Res. Commun. 140, 1007-1014.
Brunori, M., Bickar, D., Bonaventura, J., and Bonaventura, C. (1985). J. Biol. Chem. 260, 7165-7167.
Callahan, P. M., and Babcock, G. T. (1983). Biochemistry 22, 452-461.
Copeland, R. A. (1991). Proc. Natl. Acad. Sci. USA 88, 7281-7283.
Cowan, J. A., Upmacis, R. K., Beratan, D. N., Onuchic, J. N., and Gray, H. B. (1988). Ann. N.Y. Acad. Sci. 550, 68-84.
Einarsdóttir, O., and Caughey, W. S. (1984). Biochem. Biophys. Res. Commun. 124, 836-842.
Farrar, J. A., Thomson, A. J., Cheesman, M. R., Dooley, D. M., and Zumft, W. G. (1991). FEBS Lett. 294, 11-15.
Gouterman, M. (1959). J. Chem. Phys. 30, 1139-1161.
Greenwood, C., Hill, B. C., Barber, D., Eglinton, D. G., and Thomson, A. J. (1983). Biochem. J. 215, 303-316.
Greenwood, C., Thomson, A. J., Barrett, C. P., Peterson, J., George, G. N., Fee, J. A., and Reichardt, J. (1988). Ann. N.Y. Acad. Sci. 550, 47-52.
Hendler, R. W., and Westerhoff, H. V. (1992). Biophys. J. 63, 1586-1604.
Hendler, R. W., Reddy, K. V. S., Shrager, R. I., and Caughey, W. S. (1986). Biophys. J. 49, 717-729.
Horie, S. (1964). J. Biochem. 56, 67-71.
Iwata, S., Ostermeier, C., Ludwig, B., and Michel, H. (1995). Nature 376, 660-669.
Jones, G. D., Jones, M. G., Wilson, M. T., Brunori, M., Colosimo, A., and Sarti, P. (1983). Biochem. J. 209, 175-182.
Kadenbach, B., and Merle, P. (1981). FEBS Lett. 135, 1-11.
Keilin, D., and Hartree, E. F. (1939). Proc. R. Soc. London B127, 167-191.
Kobayashi, K., Une, H., and Hayashi, K. (1989). J. Biol. Chem. 264, 7976-7980.
Lambeth, D. O., and Palmer, G. (1973). J. Biol. Chem. 248, 6095-6103.
Lappalainen, P., and Saraste, M. (1994). Biochim. Biophys. Acta Bio-Energetics 1187, 222-225.
Lappalainen, P., Aasa, R., Malmström, B. G., and Saraste, M. (1993). J. Biol. Chem. 268, 26416-26421.
Lemberg, R., and Barrett, J. (1973). Cytochromes. Academic Press, London and New York.
Lemberg, R., and Mansley, G. E. (1966). Biochim. Biophys. Acta 118, 19-35.
Liao, G. L., and Palmer, G. (1996). Biochim. Biophys. Acta 1274, 109-111.
Malmström, B. G. (1974). Q. Rev. Biophys. 6, 389-431.
Marcus, R. A., and Sutin, N. (1985). Biochim. Biophys. Acta 811, 265-322.
Miki, T., and Orii, Y. (1986). J. Biochem. 100, 735-745.
Moody, A. J. (1996). Biochim. Biophys. Acta Bio-Energetics 1276, 6-20.
Moser, C. C., Keske, J. M., Warncke, K., Farid, R. S., and Dutton, P. L. (1992). Nature 355, 796-802.
Nicholls, P., and Wrigglesworth, J. M. (1988). Ann. N.Y. Acad. Sci. 550, 59-67.
Orii, Y. (1984). J. Biol. Chem. 259, 7187-7190.
Orii, Y. (1993). Biochemistry 32, 11910-11914.
Orii, Y., and Anni, H. (1990). FEBS Lett. 267, 117-120.
Orii, Y., Manabe, M., and Yoneda, M. (1977). J. Biochem. 81, 505-517.
Reddy, K. V. S., Hendler, R. W., and Bunow, B. (1986). Biophys. J. 49, 705-715.
Sherman, D., Kotake, S., Ishibe, N., and Copeland, R. A. (1991). Proc. Natl. Acad. Sci. USA. 88, 4265-4269.
Slutter, C. E., Sanders, D., Wittung, P., Malmström, B. G., Aasa, R., Richards, J. H., Gray, H. B., and Fee, J. A. (1996). Biochemistry 35, 3387-3395.
Snyder, S. H. (1992). Science 257, 494-496.
Steffens, G. C. M., Biewald, R., and Buse, G. (1987). Eur. J. Biochem. 164, 295-300.
Tsudzuki, T., Orii, Y., and Okunuki, K. (1967). J. Biochem. 62, 37-45.
Tsukihara, T., Aoyama, H., Yamashita, E., Tomizaki, T., Yamaguchi, H., Shinzawa-Itoh, K., Nakashima, R., Yaono, R., and Yoshikawa, S. (1995). Science 269, 1069-1074.
Tsukihara, T., Aoyama, H., Yamashita, E., Tomizaki, T., Yamaguchi, H., Shinzawa-Itoh, K., Nakashima, R., Yaono, R., and Yoshikawa, S. (1996). Science 272, 1136-1144.
Vanneste, W. H. (1966). Biochemistry 5, 838-848.
Von Wachenfeldt, C., de Vries, S., and Van der Oost, J. (1994). FEBS Lett. 340, 109-113.
Warburg, O., and Negelein, E. (1928). Biochem. Z. 202, 202-228.
Wikström, M., Krab, K., and Saraste, M. (1981). Cytochrome Oxidase A Synthesis Academic Press, London and New York.
Wilson, D. F., and Dutton, P. L. (1970). Arch. Biochem. Biophys. 136, 583-584.
Yonetani, T. (1960). J. Biol. Chem. 235, 845-852.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Orii, Y. Determination and Novel Features of the Absolute Absorption Spectra of the Heme a Moieties in Cytochrome c Oxidase. J Bioenerg Biomembr 30, 47–53 (1998). https://doi.org/10.1023/A:1020555427215
Issue Date:
DOI: https://doi.org/10.1023/A:1020555427215