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The toxicity induced by phosphinothricin and methionine sulfoximine in rice leaves is mediated through ethylene but not abscisic acid

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Abstract

The possible role of ethylene and abscisic acid (ABA) in regulating thetoxicity of detached rice leaves induced by phosphinothricin (PPT) andmethionine sulfoximine (MSO), both known to be glutamine synthetase (GS)inhibitors, was studied. During 12 h of incubation, PPT and MSOinhibited GS activity, accumulated NH4 + and inducedtoxicity of detached rice leaves in the light but not in darkness. PPT and MSOtreatments also resulted in an increase of ethylene production and ABA contentin a light dependent way. Addition of fluridone, an inhibitor of ABAbiosynthesis, reduced ABA content in rice leave but did not preventNH4 + toxicity of rice leaves induced by PPT and MSO.Cobalt ion, an inhibitor of ethylene biosynthesis, affected PPT- andMSO-inducedtoxicity of detached rice leaves but had no effect on PPT- and MSO-inducedNH4 + accumulation. Results suggest that ethylene but notABA may be responsible for PPT- and MSO-induced toxicity of detached riceleaves.

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Authors and Affiliations

  1. Department of Agronomy, National Taiwan University, Taipei, Republic of China

    Yu-Chang Tsai, Chien Teh Chen, Yi Ting Hsu & Ching Huei Kao

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  1. Yu-Chang Tsai
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  2. Chien Teh Chen
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  3. Yi Ting Hsu
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  4. Ching Huei Kao
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Correspondence to Ching Huei Kao.

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Tsai, YC., Chen, C.T., Hsu, Y.T. et al. The toxicity induced by phosphinothricin and methionine sulfoximine in rice leaves is mediated through ethylene but not abscisic acid. Plant Growth Regulation 37, 167–176 (2002). https://doi.org/10.1023/A:1020537108046

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