Abstract
Fluorescence kinetics of perylene molecules in hemoglobin-free human erythrocyte membranes is investigated as a function of the refractive index of the external medium varied by adjusting the concentration of sorbitol or sucrose in an aqueous suspension of erythrocyte ghosts. It has been found that the fluorescence of perylene in erythrocyte ghosts decays nonexponentially, with the mean decay time decreasing from 7.13 to 5.70 ns with an increase in the refractive index of the suspension from 1.333 to 1.442. An analysis of the dependence made it possible to obtain an estimate of the second-rank orientational order parameter of perylene in the human erythrocyte membrane «P 2 (cosθ)» = 0.32 ... 0.43, which bears witness of considerable ordering of perylene molecules along acyl chains of phospholipids constituting the membrane. Good correspondence of the order parameter with the value of the steady-state emission anisoptropy of perylene in erythrocyte ghosts suggests that acyl chains of phospholipids in the human erythrocyte membrane are predominantly oriented along the normal to its surface.
Similar content being viewed by others
REFERENCES
E. M. Purcell (1946) Phys. Rev. 69, 681.
C. M. Soukoulis (Ed.) (1993) Photonic Bandgaps and Localization, Plenum Press, New York.
E. Burstein and C. Weisbuch (Eds.) (1995) Confined Electrons and Photons: New Physics and Applications, Plenum Press, New York.
W. Lukosz (1980) Phys. Rev. B 22, 3030–3038.
H. Khosravi and R. Loudon (1991) Proc. R. Soc. London Ser. A 433, 337–352; 436, 373–389.
H. P. Urbach and G. L. J. A. Rikken (1998) Phys. Rev. A 57, 3913–3930.
J. K. Trautman, J. J. Macklin, T. D. Harris, and L. E. Brus (1996) Science 272, 255–258.
R. E. Kunz and W. Lukosz (1980) Phys Rev. B 21, 4814–4828.
G. L. J. A. Rikken (1995) Phys. Rev. A 51, 4906–4909.
A. N. Rubinov and V. I. Nikolaev (1970) Izv. Akad. Nauk SSSR Ser. Fiz. 34, 1308–1311.
F. De Martini, G. Innocenti, G. R. Jakobowitz, and P. Mataloni (1987) Phys. Rev. Lett. 59, 2955–2958.
M. D. Barnes, W. B. Whitten, S. Arnold, and J. M. Ramsey (1992) J. Chem. Phys. 97, 7842–7845.
P. Lavallard, M. Rosenbauer, and T. Gacoin (1996) Phys. Rev. A 54, 5450–5453.
J. Martorell and N. M. Lawandy (1990) Phys. Rev. Lett. 65, 1877–1880.
E. P. Petrov, V. N. Bogomolov, I. I. Kalosha, and S. V. Gaponenko (1998) Phys. Rev. Lett. 81, 77–80.
D. Toptygin, J. Svobodova, I. Konopasek, and L. Brand (1992) J. Chem. Phys. 96, 7919–7930.
D. Toptygin and L. Brand (1993) Biophys. Chem. 48, 205–220.
D. Toptygin and L. Brand (1995) J. Fluoresc. 5, 39–50.
M. Cho (1997) J. Chem. Phys. 107, 4499–4506.
E. Gratton and T. Parasassi (1995) J. Fluoresc. 5, 51–57.
M. M. G. Krishna and N. Periasamy (1998) J. Fluoresc. 8, 81–92.
U. Cogan, M. Shinitzky, G. Weber, and T. Nishida (1973) Biochemistry 12, 521–528.
D. Papahadjopoulos, K. Jacobson, S. Nir, and T. Isac (1973) Biochim. Biophys. Acta 311, 330–348.
I. B. Berlman (1965) Handbook of Fluorescence Spectra of Aromatic Molecules, Academic Press, New York and London.
M. van Gurp, T. van Heijnsbergen, G. van Ginkel, and Y. K. Levine (1989) J. Chem. Phys. 90, 4103–4111.
J. Szubiakowski, A. Balter, W. Nowak, A. Kowalczyk, K. Wiśniewski, and M. Wierzbowska (1996) Chem. Phys. 208, 283–296.
G. E. Dobretsov (1989) Fluorescent Probes in the Study of Cells, Membranes, and Lipoproteins, Nauka, Moscow [in Russian].
J. S. Beck (1978) J. Theor. Biol. 75, 487–501.
M. R. Lieber and T. L. Steck (1982) J. Biol. Chem. 257, 11651–11659, 11660–11666.
R. Peters (1973) Biophys. Biochim. Acta 318, 469–473.
V. G. Ivkov and G. N. Berestovskii (1982) Lipid Bilayer of Biological Membranes, Nauka, Moscow [in Russian].
G. Guidotti (1972) Annu. Rev. Biochem. 41, 731–752.
G. Lipari and A. Szabo (1980) Biophys. J. 30, 489–506.
C. Zannoni (1981) Mol. Phys. 42, 1303–1320.
Y. Jiang and G. L. Blanchard (1994) J. Phys. Chem. 98, 6436–6440.
A. A. Boldyrev, S. V. Kotelevtsev, M. E. Lanio, K. Alvarez, and P. Perez (1990) Introduction to Biomembranology, Moscow University Publishers, Moscow [in Russian].
J. T. Dodge, C. Mitchell, and D. J. Hanahan (1963) Arch. Biochem. Biophys. 100, 119–130.
Z. I. Lalchev and K. S. Birdi (1988) C. R. Acad. Bulg. Sci. 41, 49–51.
M. A. K. Markwell, S. M. Haas, L. L. Bieber, and N. E. Tolbert (1978) Anal. Biochem. 87, 206–210.
R. M. C. Dawson, D. C. Elliot, W. H. Elliott, and K. M. Jones (1986) Data for Biochemical Research, 3rd ed., Clarendon Press, Oxford.
A. Siemiarczuk, B. D. Wagner, and W. R. Ware (1990) J. Phys. Chem. 94, 1661–1666.
D. M. Gakamsky, A. A. Goldin, E. P. Petrov, and A. N. Rubinov (1992) Biophys. Chem. 44, 47–60; Erratum (1992) Biophys. Chem. 45, 194–195.
E. P. Petrov (1997) in Proceedings of the Vth Internstional Conference on Methods and Applications of Fluorescence Spectroscopy, Köster, Berlin, Paper P142.
J. Večer, A. A. Kowalczyk, and R. E. Dale (1993) Rev. Sci. Instrum. 64, 3403–3412.
N. Ostrowsky, D. Sornette, P. Parker, and E. R. Pike (1981) Opt. Acta 28, 1059–1070.
J. Requena and D. A. Haydon (1975) Proc. R. Soc. Lond. A 347, 161–177.
R. J. Cherry, K. Hsu, and D. Chapman (1972) Biochim. Biophys. Acta 288, 12–21.
R. Peters (1971) Biochim. Biophys. Acta 233, 465–468.
L. G. Astaf'eva and G. P. Ledneva (1998) Personal communication.
M. Shinitzky and Y. Barenholz (1974) J. Biol. Chem. 249, 2652–2657.
E. Prenner, A. Hermetter, G. Landl, H. Stütz, H. F. Kauffmann, and A. J. Kungl (1993) J. Phys. Chem. 97, 2788–2792.
M. Kehry, J. Yguerabide, and S. J. Singer (1976) Science 195, 486–487.
B. Rudy and C. Gitler (1972) Biochim. Biophys. Acta 288, 231–236.
M. B. Feintein, S. M. Fernandez, and R. I. Sha'afi (1975) Biochim. Biophys. Acta 413, 354–370.
J. R. Lakowicz and J. R. Knutson (1980) Biochemistry 19, 905–911.
C. Karolis, H. G. L. Coster, T. C. Chilcott, and K. D. Barrow (1998) Biochim. Biophys. Acta 1368, 247–255.
A. S. Holmes, D. J. S. Birch, K. Suhling, R. E. Imhof, T. Salthammer, and H. Dreeskamp (1991) Chem. Phys. Lett. 186, 189–194.
P. L.-G. Chong, B. W. van der Meer, and T. E. Thompson (1985) Biochim. Biophys. Acta 813, 253–265.
D. M. Gakamsky, A. P. Demchenko, N. A. Nemkovich, A. N. Rubinov, V. I. Tomin, and N. V. Shcherbatska (1992) Biophys. Chem. 42, 49–61.
E. P. Petrov and A. N. Rubinov (1994) Lietuvos Fizikos Žurnalas 34, 47–51.
A. N. Tikhonov and V. Ya. Arsenin (1986) Methods for Solving Ill-Posed Problems, 3rd ed., Nauka, Moscow [in Russian].
C. L. Lawson and R. J. Hanson (1974) Solving Least Squares Problems, Prentice-Hall, Englewood Cliffs, NJ.
M. Bertero, P. Boccacci, and E. R. Pike (1982) Proc. R. Soc. Lond. A 383, 15–29.
M. Bertero, C. De Mol, and E. R. Pike (1985) in W.-M. Boerner, H. Brand, L. A. Cram, D. T. Gjessing, A. K. Jordan, W. Keydel, G. Schweirz, and M. Vogel (Eds.), Inverse Methods in Electromagnetic Imaging, Part I, D. Reidel, Dordrecht, pp. 319–328.
G. Wahba (1977) SIAM J. Numer. Anal. 14, 651–667.
G. H. Golub, M. Heath, and G. Wahba (1979) Technometrics 21, 215–223.
P. Geladi and B. R. Kowalski (1986) Anal. Chim. Acta 185, 1–17.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Petrov, E.P., Kruchenok, J.V. & Rubinov, A.N. Effect of the External Refractive Index on Fluorescence Kinetics of Perylene in Human Erythrocyte Ghosts. Journal of Fluorescence 9, 111–121 (1999). https://doi.org/10.1023/A:1020524832493
Issue Date:
DOI: https://doi.org/10.1023/A:1020524832493