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Activation of Mononuclear Cells by Interleukin-12: An In Vivo Study in Chimpanzees

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Abstract

Interleukin (IL)-12 is considered a central regulator of host resistance against a variety of pathogens. Therefore, IL-12 has been advocated as a potential therapeutic agent in infections. To determine the in vivo effects of IL-12 on mononuclear cells involved in the host immune response, four chimpanzees received an intravenous injection of recombinant IL-12 (1 μg/kg). IL-12 induced a sustained decrease in lymphocyte counts, with decreases in CD3+/CD4+ and CD3+/CD8+ cells, while monocyte counts showed a transient increase. IL-12 injection resulted in a shift toward a Th1-mediated immune response as indicated by increased interferon-γ production during whole-blood stimulation, while not influencing IL-4 production. IL-12-induced activation of NK cells and phagocytes, as indicated by increased NK cell cytotoxicity and increased plasma levels of granzymes A and B and of chitotriosidase activity. These data support the hypothesis that IL-12 may serve as a useful therapeutic agent in infections where a cell-mediated response is protective.

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Authors and Affiliations

  1. Laboratory of Experimental Internal Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands

    Fanny N. Lauw, Anje A. Te Velde, Pascale E. P. Dekkers, Sander J. H. Van Deventer & Tom Van Der Poll

  2. Department of Infectious Diseases, Tropical Medicine and AIDS, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands

    Fanny N. Lauw, Peter Speelman & Tom Van Der Poll

  3. Department of Biochemistry, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands

    Johannes M. F. G. Aerts

  4. Department of Pathophysiology of Plasma Proteins, Central Laboratory of The Netherlands Red Cross Blood Transfusion Service, Amsterdam, The Netherlands

    C. Erik Hack

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  1. Fanny N. Lauw
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Lauw, F.N., Te Velde, A.A., Dekkers, P.E.P. et al. Activation of Mononuclear Cells by Interleukin-12: An In Vivo Study in Chimpanzees. J Clin Immunol 19, 231–238 (1999). https://doi.org/10.1023/A:1020520130792

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