Abstract
The use of a newly synthesized fluorescent probe, ABM (conditional name), to determine changes of membrane composition (fluidity), biomass yield, ethanol productivity, and yield of Zymomonas mobilis under anaerobic conditions was investigated. A strong correlation between ABM spectral characteristics in cell suspension and all obtained parameters was observed. It was concluded that application of ABM is a useful tool to detect temperature-induced changes in the membrane composition of Zymomonas mobilis.
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REFERENCES
I. Swing and I. DeLay (1997) Bacteriol. Rev. 4, 1–46.
I. Laudrin and G. Goma (1982) Biotechnol. Lett. 4, 537–542.
K. Ohta, K. Supanwong, and S. Hayashida (1981) J. Ferment. Technol. 59, 435–439.
W. R. Finnerty and R. A. Makula (1975) Crit. Rev. Microbiol. 4, 1–40.
P. J. Qulinn and D. Chapmann (1980) Crit. Rev. Biochem 8, 1–117.
J. R. Lakowicz (1983) Principles of Fluorescence Spectroscopy, Plenum Press, New York.
M. Shinitzky (1978) Biochim. Biophys. Acta 515, 367–394.
H. S. Mann and D. R. Whitney (1974) Ann. Math. Stat. 18, 50.
D. B. Duncan (1970) Biometrics 26, 141.
I. Kalnina, I. Meirovics, and E. Rashkina (1996) Functional Materials, Institute for Single Crystals, Kharkov, Ukraine, Vol. 3(4), pp. 551–555.
I. Kalnina, I. Meirovics, and J. Rashkina (1997) in NATO Advanced Workshop on Synthesis, Optical Properties, and Applications of Near-Infrared (NIR) Dyes in High Technology Fields, Trest, Czech Republic, P. 20.
I. Kalnina and I. Meirovics (1999) J. Fluoresc. 9, 27–32.
H. Kruckova, M. Pilmane, I. Kalnina, I. Meirovics, and V. Scripchanova (1996) Eur. Respir. J. 9(Suppl. 23):335.
A. S. Benschoter and L. O. Ingram (1986) Appl. Environ. Microbiol. 51(6), 1278–1284.
G. E. Dobretsov (1983) Fluorescent Probes in Investigation of Cells, Membranes and Lipoproteins, Moscow, Nauka.
L. G. Kokina, G. E. Dobretsov, G. Walzel, E. M. Kogan, Y. I. Zimin, and A. Vladimirov (1981) J. Immunol. Methods 45(2), 227–237.
M. Shinitzky (1984) Physiology of Membrane Fluidity, CRC Press, Boca Raton, Vol. 1, p. 1–52.
A. V. Izvekova (1991) Sucess Contemp. Biol. 111, 577–591.
I. A. Huber, G. B. Xu, G. Juergens, E. Buhler, K. F. Gey, D. Schonitzer, K. N. Trail, and G. Wick (1991) Eur. J. Immunol. 21, 2761–2765.
M. Sinensky (1974) Proc. Natl. Acad. Sci. USA 71, 522–525.
J. E. Cronan and P. R. Vagelos (1972) Biochim. Biophys. Acta 286, 5–60.
C. R. Raetz (1978) Microbiol. Rev. 3, 614–659.
A. J. De Siervo (1969) J. Bacteriol. 100, 1347–1349.
K. M. Dombek and L. O. Ingram (1984) J. Bacteriol. 157, 233–239.
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Kalnina, I., Toma, M.M. & Meirovics, I. Temperature-Induced Changes in the Membrane Fluidity of Zymomonas Mobilis: Study Using a New Flourescent Probe, ABM. Journal of Fluorescence 9, 367–370 (1999). https://doi.org/10.1023/A:1020500411054
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DOI: https://doi.org/10.1023/A:1020500411054