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Hydrophobized Formate Dehydrogenase from Pseudomonas sp. 101 in the System of Reverse Micelles of Aerosol OT in Octane

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Abstract

NAD+-dependent formate dehydrogenase (FDH) was hydrophobized with palmitoyl chloride to give the samples with various modification degrees (2–10). The native and modified FDHs were comparatively studied in the system of reverse micelles of Aerosol OT in octane. Like the native, the modified enzyme displayed three maxima in the curve of dependence of its catalytic activity on the degree of surfactant hydration (the micelle size), which reflect the enzyme functioning in the form of a monomer, dimer, or octamer. The peak corresponding to the functioning of the FDH dimer was found to decrease along with an increase in the modification degree. Thus, the modified enzyme mainly functions in the form of monomer and octamer. The modified FDH displayed membranotropy and revealed the dependence of catalytic activity on surfactant concentration.

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Authors and Affiliations

  1. Faculty of Chemistry, Moscow State University, Vorob'evy gory, Moscow, 119899, Russia

    D. N. Trofimova (Chair of Chemical Enzymology) & A. V. Levashov

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  1. D. N. Trofimova
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  2. A. V. Levashov
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Correspondence to A. V. Levashov.

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Trofimova, D.N., Levashov, A.V. Hydrophobized Formate Dehydrogenase from Pseudomonas sp. 101 in the System of Reverse Micelles of Aerosol OT in Octane. Russian Journal of Bioorganic Chemistry 28, 392–396 (2002). https://doi.org/10.1023/A:1020420027926

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