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Selenoprotein W gene regulation by selenium in L8 cells

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Abstract

The effects of selenium on selenoprotein W gene expression were examined in cultured L8 rat skeletal muscle cells. Selenoprotein W contains selenium as selenocysteine in the primary protein structure and levels of this selenoprotein are affected by selenium. Northern blots indicated that there were no significant changes (P<0.05) in selenoprotein W mRNA levels during cell proliferation and differentiation. Reduction of selenium concentration in the medium decreased the selenoprotein W mRNA levels. Nuclear run-on experiments with isolated L8 nuclei showed the same rate of selenoprotein W mRNA synthesis in cells cultured in either low selenium or selenium supplemented medium, suggesting that the transcription rate of the selenoprotein W gene is independent of selenium. Measurement of the selenoprotein W mRNA half-life in myoblasts treated with the transcription inhibitor, α-amanitin, showed that selenoprotein W mRNA levels decreased over time with an estimated half-life of 57 h for cells grown in low selenium medium. Selenium treatment increased the selenoprotein W mRNA half-life 2-fold. These data suggest that selenium stabilizes selenoprotein W mRNA but has no effect on transcription.

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Author notes

  1. Q.P. Gu

    Present address: Johns Hopkins University, Cardiology Ross 312, 1721 East Madison Street, Baltimore, MD, 21205-1151, USA

Authors and Affiliations

  1. Departments of Environmental and Molecular Toxicology, Oregon State University, Corvallis, Oregon, 97331, USA

    Q.P. Gu & P.D. Whanger

  2. Microbiology, Oregon State University, Corvallis, Oregon, 97331, USA

    W. Ream

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  1. Q.P. Gu
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  2. W. Ream
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  3. P.D. Whanger
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Gu, Q., Ream, W. & Whanger, P. Selenoprotein W gene regulation by selenium in L8 cells. Biometals 15, 411–420 (2002). https://doi.org/10.1023/A:1020241032336

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