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Effects of Prostaglandin E2 on Gastric Irritant-Induced Apoptosis

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Abstract

We previously reported that various gastric irritants induced both apoptosis and necrosis in cultured gastric mucosal cells. In a continuation of this work, the present study has examined the effects of prostaglandin E2 (PGE2), a cytoprotective factor for gastric mucosa in vivo, on gastric irritant-induced apoptosis and necrosis in vitro. PGE2 inhibited ethanol-induced apoptosis and increased cell viability in a dose-dependent manner in primary cultures of guinea pig gastric mucosal cells. PGE2 also inhibited hydrogen peroxide-induced apoptosis. In contrast, PGE2 showed no cytoprotective effects against ethanol-induced necrosis. Based on these results, we consider that the cytoprotective effects of PGE2 on gastric mucosa in vivo can be partially explained by its inhibitory effect on gastric irritant-induced apoptosis.

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Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, Okayama University, Okayama, 700-8530, Japan

    Tatsuya Hoshino, Tatsunori Takano, Shinji Tsutsumi, Wataru Tomisato, Tomofusa Tsuchiya & Tohru Mizushima

  2. PRESTO, Japan Science and Technology Corporation, Okayama, 700-8530, Japan

    Tatsuya Hoshino, Tatsunori Takano, Shinji Tsutsumi, Wataru Tomisato, Tomofusa Tsuchiya & Tohru Mizushima

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  1. Tatsuya Hoshino
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  2. Tatsunori Takano
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  3. Shinji Tsutsumi
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  4. Wataru Tomisato
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  5. Tomofusa Tsuchiya
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  6. Tohru Mizushima
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Hoshino, T., Takano, T., Tsutsumi, S. et al. Effects of Prostaglandin E2 on Gastric Irritant-Induced Apoptosis. Dig Dis Sci 47, 2370–2379 (2002). https://doi.org/10.1023/A:1020164000898

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