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Further evaluation of amniotic membrane banking for transplantation in ocular surface diseases

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Abstract

Objective: To define the best conditions foramniotic membrane preparation, storage and banking in its use for cornealreconstruction.Methods: Amniotic membrane pieces were prepared understerile conditions from placentas selected on the basis of donor medical andsocial history, serology, microbiological tests and histology. The pieces werekept at −140 °C but before grafting they werethawed and stored at 4 °C in RPMI medium, to have apreparation usable within 72 h. This procedure was validatedby testing its therapeutic effectiveness in 25 patients 13 of which had cornealulcers of various origin, 3 had sequelae of herpes simplex keratitis, 3 bandkeratopathy and 6 corneal stem cell deficiency due to chemical or thermalburns.Results: The preparation showed appreciableanti-inflammatory and analgesic effects. In the absence of corneal stem celldeficiency a stable re-epithelialisation was achieved in 15 out of 19 patients.When the limbus was lesioned, the amniotic membrane decreased vascularizationand increased the number of corneal epithelial cells only in 1 of the 6patients. No adverse reactions attributable to the tissue were recorded.Conclusions: A ready-to-use amniotic membrane preparationstored at 4 °C after cryopreservation has been tested incorneal reconstruction. Like the amniotic membrane thawed immediately beforegrafting, this preparation displayed full therapeutic effect in epithelialdefects with stromal ulceration but without severe limbal stem cell deficiency.In two years banking activity 463 pieces of the preparation were successfullydistributed to 90 Italian hospitals.

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Authors and Affiliations

  1. Division of Ophthalmology, Hospital “SS Giovanni e Paolo', Venezia, Italy

    Paolo Rama & Roberta Giannini

  2. Department of Pharmacology and Anesthesiology, University of Padova, Padova, Italy

    Alessandro Bruni

  3. Regional Tissue Bank ULSS 9, Treviso, Italy

    Claudio Gatto

  4. Fondazione Banca degli Occhi del Veneto, Via Felisati 109, 30174, Venezia, Italy

    Roberta Tiso & Diego Ponzin

Authors
  1. Paolo Rama
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  2. Roberta Giannini
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  3. Alessandro Bruni
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  4. Claudio Gatto
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  5. Roberta Tiso
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  6. Diego Ponzin
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Rama, P., Giannini, R., Bruni, A. et al. Further evaluation of amniotic membrane banking for transplantation in ocular surface diseases. Cell Tissue Banking 2, 155–163 (2001). https://doi.org/10.1023/A:1020158206073

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