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Effect of Chronic O3 Fumigation on the Activity of Some Calvin Cycle Enzymes in Two Poplar Clones

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Photosynthetica

Abstract

The effects of long-term exposure to ozone (O3, 60 mm3 m-3 for 5 h d-1) on some Calvin cycle enzymes, in particular those modulated by the thioredoxin system, were studied in two poplar clones. These clones differ in sensitivity to O3. In the I-214 clone, the first effects from O3 treatment were seen after 40 d of fumigation, while the Eridano clone showed visible symptoms of damage after only 15 d of the treatment. Specific activity of ribulose-1,5-bisphosphate carboxylase/oxygenase (E.C. 4.1.1.39) diminished in both the clones, while specific activity of phosphoenolpyruvate carboxylase (E.C. 4.1.1.31) increased. Exposure to O3 also caused a reduction in the specific activity of ribulose-1,5-bisphosphate kinase (E.C. 2.7.1.19) in both clones. At the end of the exposure to O3, specific activity of glyceraldehyde 3-phosphate dehydrogenase (E.C. 1.2.1.13) increased in I-214 and remained similar to the control in Eridano, whereas specific activity of fructose-1,6-bisphosphate phosphatase (E.C. 3.1.3.11) was higher in Eridano and similar to the control in I-214.

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Authors and Affiliations

  1. Dipartimento di Chimica e Biotecnologie Agrarie, Università di Pisa, Via del Borghetto 80, 56124, Pisa, Italy

    E. Degl'Innocenti, L. Guidi & G.F. Soldatini

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  1. E. Degl'Innocenti
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  2. L. Guidi
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  3. G.F. Soldatini
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Degl'Innocenti, E., Guidi, L. & Soldatini, G. Effect of Chronic O3 Fumigation on the Activity of Some Calvin Cycle Enzymes in Two Poplar Clones. Photosynthetica 40, 121–126 (2002). https://doi.org/10.1023/A:1020127231198

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