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Calorimetric study of the effects of 2,4-dichlorophenol on the thermotropic phase behavior of DPPC liposomes

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Abstract

The effect of 2,4-dichlorophenol (DCP) on the main transition and pretransition of fully hydrated (20 mass%) dipalmitoylphosphatidylcholine (DPPC) multilamellar liposomes has been studied by differential scanning calorimetry (DSC). It was observed that an increase in the molar ratio of DCP/DPPC (from 4·10-5 up to 2·10-2) causes progressive reductions in the temperature and enthalpy of the pretransition. The higher concentration of DCP eliminates the pretransition. The influence of DCP on the main transition in this molar ratio range is not drastic, but a decrease in temperature and in the enthalpy values was observed. In the molar ratio range (from 2·10-1 up to 4·10-1) the DSC scans show multiple main transition peaks instead of the characteristic single peak of the main transition. Above a DCP/DPPC molar ratio of 0.6 a new peak appears at 25°C having about the same transition enthalpy as the main transition of the pure system.

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References

  1. S. Mabrey and J. M. Sturtevant, Methods in Membrane Biology, 9 (1978) 237.

    Google Scholar 

  2. A. Blume, Thermochim. Acta, 193 (1991) 299.

    Google Scholar 

  3. A. Tardieu, V. Luzatti and F. C. Reman, J. Mol. Biol., 75 (1973) 711.

    Google Scholar 

  4. M. J. Ruocco and G. G. Shipley, Biochim. Biophys. Acta, 684 (1982) 59.

    Google Scholar 

  5. M. J. Ruocco and G. G. Shipley, Biochim. Biophys. Acta, 691 (1982) 309.

    Google Scholar 

  6. P. R. Maulik, M. J. Ruocco and G. G. Shipley, Chem. Phys. of Lipids, 45 (1990) 123.

    Google Scholar 

  7. G. Lipka, B. Z. Chowdhry and J. M. Sturtevant, J. Phys. Chem., 88 (1984) 5401.

    Google Scholar 

  8. A. Bota and M. Kriechbaum, Coll. Surf. A., 141 (1998) 441.

    Google Scholar 

  9. A. Bota, T. Drucker, M. Kriechbaum, Zs. Palfi and G. Rez, Langmuir, 15 (1998) 3101.

    Google Scholar 

  10. P. Steinle, Ph. Thalmann, P. Höhener, K. W. Hanselmann and G. Stucki, Environ. Sci. Technol., 34 (2000) 771.

    Google Scholar 

  11. Y. Beaton, L. Z. Shaw, L. A. Glover, A. A. Meharg and K. Killham, Environ. Sci. Technol., 33 (1999) 4086.

    Google Scholar 

  12. W. F. Jardim, S. G. Moraes and M. M. K. Takiyama, Wat. Res., 31 (1997) 1728.

    Google Scholar 

  13. B. Escher and R. P. Schwarzenbach, Environ.Sci. Technol., 30 (1996) 260.

    Google Scholar 

  14. B. Escher, R. Hunziker and R. P. Schwarzenbach, Environ.Sci. Technol., 33 (1999) 560.

    Google Scholar 

  15. J. F. Nagle and D. A. Wilkinson, Biochemistry, 21 (1982) 3817.

    Google Scholar 

  16. S. T Nagle, T. Moore, H. I. Petrache and J. F. Nagle, Biochim. Biophys. Acta, 19 (1998) 1369.

    Google Scholar 

  17. Á. Csiszár, A. Bóta, Cs. Novák, E. Klumpp and G. Subklew, Progr. Coll. Polym. Sci., 117 (2001) 145.

    Google Scholar 

  18. A. Ambrosini, G. Bossi, S. Dante and B. Dubini, Chem. Phys. Lipids, 95 (1998) 37.

    Google Scholar 

  19. R. N. McElhaney, Chem. Phys. Lipids, 30 (1982) 229.

    Google Scholar 

  20. M. K. Jain and N. M. Wu, J. Membr. Biol., 34 (1977) 157.

    Google Scholar 

  21. R. A. Videira, M. C. Antunes-Madeira and V. M. C. Madeira, Biochim. Biophys. Acta, 1419 (1999) 151.

    Google Scholar 

  22. H. Nagase, H. Ueda and M. Nakagaki, Biochim. Biophys. Acta, 1371 (1998) 223.

    Google Scholar 

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Authors and Affiliations

  1. Department of Physical Chemistry, Budapest University of Technology and Economics, Budapest, Hungary

    Á. Csiszár & A Bóta

  2. Department of General and Analytical Chemistry, Budapest University of Technology and Economics, Budapest, Hungary

    Cs. Novák

  3. Research Center Jülich, Institute of Agrosphere, Germany

    Á. Csiszár & E. Klumpp

  4. Research Center Jülich, Institute of Physical Chemistry, Germany

    G. Subklew

Authors
  1. Á. Csiszár
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  2. A Bóta
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  3. Cs. Novák
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  4. E. Klumpp
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  5. G. Subklew
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Correspondence to A Bóta.

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Csiszár, Á., Bóta, A., Novák, C. et al. Calorimetric study of the effects of 2,4-dichlorophenol on the thermotropic phase behavior of DPPC liposomes. Journal of Thermal Analysis and Calorimetry 69, 53–63 (2002). https://doi.org/10.1023/A:1019977404803

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