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Androgenetic response of heterozygous triticale populations using a greenhouse hydroponic system

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Abstract

Production of haploids, followed by chromosome doubling to produce doubled haploids (DH) represents the most rapid means of achieving complete inbreeding. In order to improve the androgenetic responses and maximize the production of green regenerants from selected plants of twenty-five triticale (× Triticosecale, Wittmack) populations (BC1F1, TC1F1, and F2) we used a uniform and optimal growth environment for anther donor plants within a greenhouse hydroponic system. Non-orthogonal analysis of deviance showed highly significant differences (p < 0.001) among populations for both induction and regeneration. The overall induction response of the populations was very high with a mean of 50.4 embryoids per 100 anthers plated. Among all tested-populations, M86-6068/TW179//EP80 (TC1F1) was the most responsive for both induction and regeneration which could be associated to its Triticum timopheevii cytoplasm. On the other hand, although populations 80465/II83-194 both as BC1F1 and F2 had a high level of induction response, only a few green plants were regenerated. These populations probably possess a partial Secale montanum genome, which could be contributing to the low regeneration ability. In conclusion, the need for optimization of donor plant growth conditions to effectively assess the androgenetic ability of individual populations/lines would be emphasized.

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References

  • Arzani, A. & N.L. Darvey, 1996. Positive effect of timopheevii cytoplasm on anther culture response of triticale. In: H. Guedes-Pinto, N.L. Darvey, V.P. Carnide (Eds.), Triticale: Today and Tomorrow, pp. 321–324, Kluwer Academic Publ., Dordrecht.

    Google Scholar 

  • Arzani, A. & N.L. Darvey, 2001. The effect of colchicine on triticale anther-derived plant: Microspore pre-treatment and haploidplant treatment using a hydroponic recovery system. Euphytica 122: 235–241.

    Article  CAS  Google Scholar 

  • Bicar, E.H. & N.L. Darvey, 1997. Development of the components of a cytoplasmic male sterility hybrid system in rye through anther culture. Euphytica 97: 151–160.

    Article  Google Scholar 

  • Bjornstad, A., H.G. Opsahl-Ferstad & M. Aasmo, 1989. Effects of donor plant environment and light during incubation on anther cultures of some spring wheat (Triticum aestivum L.) cultivars. Plant Cell Tiss Org Cult 17: 27–37.

    Google Scholar 

  • Bruins, M.B.M. & C.H.A. Snijders, 1995. Inheritance of anther culture derived green plantlet regeneration in wheat (T. aestivum). Plant Cell Tiss Org Cult 43: 13–19.

    Article  Google Scholar 

  • Charmet, G. & S. Bernard, 1984. Diallel analysis of androgenetic plant production in hexaploid triticale (xTriticosecale, Wittmack). Theor Appl Genet 69: 55–61.

    Article  Google Scholar 

  • Ekiz, H. & C.F. Konzak, 1991. Nuclear and cytoplasmic control of anther culture response in wheat: III. common wheat crosses. Crop Sci 31: 1432–1436.

    Article  Google Scholar 

  • Foroughi-Wehr, B., W. Friedt & G. Wenzel, 1982. On the genetic improvement of androgenetic haploid formation in Hordeum vulgare L. Theor Appl Genet 62: 233–239.

    Google Scholar 

  • Foroughi-Wehr, B. & G. Wenzel, 1990. Recurrent selection altering with haploid step - a rapid breeding procedure for combining agronomic traits in inbreeders. Theor Appl Genet 80: 564–568.

    Article  Google Scholar 

  • Genstat 5, 1987. Reference Manual. Oxford University Press. 749 p.

  • Gonzalez, M., I. Hernandez & N. Jouve, 1997. Analysis of anther culture response in hexaploid triticale. Plant Breed 116: 302–304.

    Article  Google Scholar 

  • Hassawi, D.S. & G.H. Liang, 1990. Effect of cultivar, incubation temperature, and stage of microspore development on anther culture in wheat and triticale. Plant Breed 105: 332–336.

    Article  Google Scholar 

  • Hoagland, D.R. & D.I. Amon, 1959. The water culture method for growing plants without soil. California Agric Exp Stn Circ 307, 32 p.

  • Immonen, S. & J. Robinston, 2000. Stress treatments and ficoll for improving green plant regeneration in triticale anther culture. Plant Sci 150: 77–84.

    Article  CAS  Google Scholar 

  • Kasha, K.J., 1989. Production of haploids in cereals. In: M. Maluszynski (Ed.), Current Options for Cereal Improvement, Kluwer Academic Publishers, pp. 71-80.

  • Lu, C.S., H.C. Sharma & H.W. Ohm, 1991. Wheat anther culture - effect of genotype and environmental conditions. Plant Cell Tiss Org Cul 24: 233–236.

    Article  Google Scholar 

  • Luckett, D.J. & N.L. Darvey, 1992. Utilisation of microspore culture in wheat and barley improvement. Aust J Bot 40: 807–828.

    Article  Google Scholar 

  • Luckett, D.J., S. Venkatanagappa, N.L. Darvey & R.A. Smithard, 1991. Anther culture of Australian wheat germplasm using modified C17 medium and membrane rafts. Aust J Plant Physiol 18: 357–367.

    Article  Google Scholar 

  • Lukjanjuk, S.F. & S.A. Ignatova, 1986. Triticale: production of haploid and homozygous plants. In: Y.P.S. Bajaj (Ed.), Biotechnology in Agriculture and Forestry, Vol. 2, Crops I, pp. 530–543, Springer, Berlin Heidelberg.

    Google Scholar 

  • McCullagh, P. & J.A. Nelder, 1987. Generalized Linear Models. 2nd edn. Chapman and Hall, U.K., 511 p.

    Google Scholar 

  • Murashige T. & S. Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497.

    Article  CAS  Google Scholar 

  • Orshinsky, B.R. & R.S. Sadasivaiah, 1997. Effect of plant growth conditions, plating density, and genotype on the anther culture response of soft white pring wheat hybrids. Plant Cell Rept 16: 758–762.

    Article  CAS  Google Scholar 

  • Ouyang, J.W. 1986. Induction of pollen plants in Triticum aestivum. In: H. Hu & H. Yang (Eds.), Haploids of Higher Plants In Vitro, pp. 26–41, Springer, Berlin Heidelberg.

    Google Scholar 

  • Ouyang, J.W., D.G. He, G.H. Feng & S.E. Jia, 1987. The response of anther culture to culture temperature varies with growth conditions of donor plants. Plant Sci 49: 145–148.

    Article  Google Scholar 

  • Rothamsted Experimental Station, 1990. Genestat 5, Release 2.2, Reference manual. Lewes Agricultural Trust. U.K.

    Google Scholar 

  • Sagi, L. & B. Barnabas, 1989. Evidence for cytoplasmic control of in vitro microspore embryogenesis in the anther culture of wheat (Triticum aestivum L.). Theor Appl Genet 78: 867–872.

    Google Scholar 

  • Schumann, G., 1990. In vitro production of haploids in triticale. In: Y.P.S. Bajaj (Ed.), Biotechnology in Agriculture and Forestry, Vol. 13, Wheat, pp. 382–402. Springer Verlag, Heidelberg.

    Google Scholar 

  • Sesek, T. & S. Dencic, 1996. The potential of anther culture technique in wheat breeding. Cereal Res Commun 24: 163–170.

    Google Scholar 

  • Torp, A.M., A.L. Hansen & S.B. Andersen, 2001. Chromosomal regions associated with green plant regeneration in wheat (Triticum aestivum L.) anther culture. Euphytica 119: 377–387.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan University of Technology, Isfahan, 84156, Iran

    Ahmad Arzani

  2. Plant Breeding Institute, Cobbitty, University of Sydney, Private Bag 11, Camden, NSW, 2570, Australia

    Norman L. Darvey

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  1. Ahmad Arzani
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  2. Norman L. Darvey
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Arzani, A., Darvey, N.L. Androgenetic response of heterozygous triticale populations using a greenhouse hydroponic system. Euphytica 127, 53–60 (2002). https://doi.org/10.1023/A:1019977304182

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