Abstract
In an acid mining lake (pH 2.6) enclosure experiments wereperformed with the addition of different concentrations oforganic carbon, nitrogen and phosphorus. SSCP-communityfingerprints, based on 16S rRNA gene amplicons, were performed tomonitor changes in the structure of the total bacterialcommunity and the sulfate reducing bacteria (SRB) in themesocosms. Total bacterial cell counts, as assessed byepifluorescence microscopy, were increased in the mesocosmsamended with organic carbon. The addition of carbon alsoincreased the number of abundant bacterial taxa substantiallyalong depth. Sulfate reducing bacteria (SRB) could be detected inall enclosures and all parts of the water column. These SRBbelonged to genus Desulfobacter as indicated bycoroborating molecular data.
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Amann, R. I., Stromley, J., Devereux, R., Key, R. and Stahl, D. A.: 1992, ‘Molecular ad micro-scopic identification of sulfate-reducing bacteria in multispecies biofilms’, Appl. Environ. Microbiol. 58, 614–623.
Bassam, B. J., Caetano-Anolles, G. and Gresshoff, P. M.: 1991, ‘Fast and Sensitive Silver Staining of DNA in Polyacrylamide Gels’, Anal. Biochem. 80, 81–84.
Brettar, I. and Höfle, M.G.: 1992, ‘Influence of ecosystematic factors on survival of Escherichia coli after large-scale release into lake water mesocosms’, Appl. Environ. Microbiol. 58, 2201–2210.
Fisher, M. M., Klug, J. L., Lauster, G., Newton, M. and Triplett, E.W.: 2000, ‘Effects of resources and trophic interactions on freshwater bacterioplankton diversity’, Microb. Ecol. 40, 125–138.
Friese, K., Wendt-Potthoff, K., Zachmann, D.W., Fauville, A., Mayer, B.and Veizer, J.: 1998, ‘Biogeochemistry of iron and sulfur in sediments of an acidic mining lake in Lusatia, Germany’, Water, Air and Soil Pollut. 108, 231–47.
Frömmichen, R.: 2001, ‘In situ Sanierungsstrategie zur Förderung der mikrobiellen Entstäuerung von geogen-schwefelsauren Bergbaurestseen - Mesokosmos-Studien’, Ph.D. Thesis, Department of Forestry-, Geo-, and Hydrosciences, Technical University Dresden, 155 pp.
Geller, W., Klapper, H. and Schultze, M.: 1998, in: W. Geller, H. Klapper and W. Salomon, (eds), Acidic Mining Lakes, Springer Verlag, 3–14.
Hard, B. C., Friedrich, S. and Babel, W.: 1997, ‘Bioremediation of acid mine water using facultatively methylotrophic metal-tolerant sulfate-reducing bacteria’, Microbiol. Res. 152, 67–73.
Herzsprung, P., Friese, K., Packroff, G., Schimmele, M., Wendt-Potthoff, K. and Winkler, M.: 1998, ‘Vertical and annual distribution of of ferric and ferrous iron in acidic mining lakes’, Acta Hydrochim. Hydrobiol. 26, 253–62.
Höfle, M. G.: 1992, ‘Bacterioplankton community structure and dynamics after large-scale release of nonidigenous bacteria as revealed by low-molecular-weight-RNA analysis’, Appl. Environ. Microbiol. 58, 3387–3394.
Höfle, M. G. and Brettar, I.: 1995, ‘Taxonomic diversity and metabolic activity of microbial communities in the water column of the Central Baltic Sea’, Limnol. Oceanogr. 40, 868–874.
Höfle, M. G., Haas, H. and Dominik, K.: 1999, ‘Seasonal dynamics of bacterioplankton community structure in a eutrophic lake as determined by 5S rRNA analysis’, Appl. Environ. Microbiol. 65, 3164–3174.
Johnson, D. B: 1998, in: W. Geller, H. Klapper and W. Salomon (eds), Acidic Mining Lakes, Springer Verlag, 285–302.
Konopka, A., Bercot, T. and Nakatsu, C.: 1999, ‘Bacterioplankton community diversity in a series of thermally stratified lakes’, Microb. Ecol. 38, 126–135.
Lebaron, P., Servais, P., Troussellier, M., Courties, C., Muyzer, G., Bernard, L., Schafer, H., Pukall, R., Stackebrandt, E., Guindulain, T. and Vives-Rego, J.: 2001, ‘Microbial community dynamics in mediterranean nutrient-enriched seawater mesocosms: changes in abundances, activity and composition’, FEMS Microbiol. Ecol. 34, 255–266.
Lessmann, D., Deneke, R., Ender, R., Hemm, M., Kapfer, M., Krumbeck, H., Wollmann, K. and Nixdorf, B.: 1999, ‘Lake Plessa 107 (Lusatia, Germany) - an extremely acidic shallow mining lake’, Hydrobiol. 409, 293–299.
Nixdorf, B., Wollmann, K. and Deneke, R.: 1998, in: W. Geller, H. Klapper and W. Salomon (eds), Acidic Mining Lakes, Springer Verlag, 147–167.
Noble, R. T. and Fuhrman, J. A.: 1998, ‘Use of SYBR green I for rapid epifluorescence counts of marine viruses and bacteria’, Aquat. Microb. Ecol. 14, 113–118.
Satake, K.: 1977, ‘Microbial sulphate reduction in a volcanic lake having pH 1.8 to 2.0’, Jpn. J. Limnol. 38, 33–35.
Schmalenberger, A., Schwieger, F. and Teppe, C. C.: 2001, ‘Effect of primers hybridizing to different evolutionarily conserved regions of the small-subunit rRNA gene in PCR-based microbial community analyses and genetic profiling’, Appl. Environ. Microbiol., 67, 3557–3563.
Schwieger, F. and Tebbe, C.: 1998, ‘A new approach to utilize PCR-single-strand-conformation polymorphism for 16s rRNA gene-based microbial community analysis’, Appl. Environ. Microbiol. 64, 4870–4876.
Shannon, P. O. and Weaver, W.: 1969, ‘The Mathematical Theory of Communication’, The University of Illinois Press Urbana, Chicago, London, Fourth edition.
Simon, M., Tilzer, M. M. and Müller, H.: 1998, ‘Bacterioplankton dynamics in a large mesotrophic lake: I abundance, production and growth control’, Arch. Hydrobiol. 143, 385–407
Weinbauer, M. G., Fritz, I., Wenderoth, D. F. and Höfle, M. G.: 2002, ‘Simultaneous extraction of total RNA and DNA from bacterioplankton suitable for quantitative structure and function analyses’, Appl. Environ. Microbiol. 68, 1082–1087.
Wollmann, K., Deneke, R., Nixdorf, B. and Packroff, G.: 2000, ‘Dynamics of planktonic food webs in three mining lakes across a pH Gradient (pH 2-4)’, Hydrobiol. 433, 3–14.
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Pöhler, I., Wenderoth, D.F., Wendt-Potthoff, K. et al. Bacterioplankton Community Structure and Dynamics in Enclosures During Bioremediation Experiments in an Acid Mining Lake. Water, Air, & Soil Pollution: Focus 2, 111–121 (2002). https://doi.org/10.1023/A:1019951612385
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DOI: https://doi.org/10.1023/A:1019951612385