Abstract
Tumour cells produce and excrete to blood many substances which are present in the cell itself in trace amounts only. Our work has been aimed at the determination of changes in electric charge and in phospholipid composition of large intestine normal mucosa and colorectal cancer cells.
Surface charge density of tumour unaffected mucosa and of tissue sections from tumours, was measured by electrophoresis. The measurements were carried out at various pH of solution. Membrane isoelectric point was determined by measuring its electric charge in function of pH as well as total positive charge at low pH and total negative charge at high pH. Qualitative and quantitative composition of phospholipids in the membrane was determined by HPLC. Four phospholipid classes were identified: PI, PS, PE and PC and their surface concentrations were determined.
The electric charge calculated from phospholipid concentrations is by three orders of magnitude higher than that determined electrophoretically. It indicates that the groups present in the membrane surface are involved in equilibria in which the charge is neutralized.
The electric charge calculated from phospholipid concentrations is by three orders of magnitude higher than that determined electrophoretically. It indicates that the groups present in the membrane surface are involved in equilibria in which the charge is neutralized.
Tumour changes provoke an increase in surface charge density of large intestine membrane, whereas the content of individual phospholipids increased or decreased depending on a patient.
Similar content being viewed by others
References
Cullis PR, Hope MJ: Physical properties and functional roles of lipids in membranes. In: D.E. Vance, J. Vance (eds). Biochemistry of Lipids, Lipoproteins and Membranes. Elsevier Science Publishers, Amsterdam, 1991, pp 1–41
Klyszejko-Stefanowicz L: Cytobiochemia PWN Warszawa 1998
Mountford CE, Wright LC: Organization of lipids in the plasma membranes of malignant and stimulated cells: A new model. Trends Biochem Sci 13: 172–177, 1988
Negendank W: Studies of human tumours by MRS: A review. NMR Biomed 5: 302–324, 1992
Gennis RB: In: C.R. Cantor (ed). Biomembranes: Molecular Structure and Functions. Springer-Verlag, New York, 1989
Dolowy K: Bioelectrochemistry of cell surfaces. Prog Surface Sci 15: 245–368, 1984
Tien HT: In: Bilayer Lipid Membranes: Theory and Practice. Marcel Dekker Inc., New York, 1974, pp 117–164
Nalecz MJ, Wojtczak L: Surface charge of biological membranes and its regulatory functions. Post Biochem 28: 191–225, 1982
Nalecz KA: Functional and structural aspects of transport of low molecular weight compounds through biological membranes. Post Biochem 35: 437–467, 1989
Ruiz-Cabello J, Cohen JS: Phospholipids metabolites as indicators of cancer cell function. NMR Biomed 226–233, 1992
Stoll BA, Secreto G: New hormone-related markers of high risk to breast cancer. Ann Oncol 3: 435–438, 1992
Krysinski P, Tien HY: Membrane electrochemistry. Prog Surf Sci 23: 17–412, 1986
Thonart Ph, Paquot M: In: D.L. Wise (ed). Bioenvironmental Systems. CRC Press Inc., Boca Raton, Florida, 1987, pp 75–98
Pigon K, Ruziewicz Z: In: Physical Chemistry, 2nd ed. PWN Warsaw 1981, pp 340–342
Rodriguez-Vico F, Martinez-Cayuela M, Zafra MF, Garcia-Peregrin E, Ramirez H: A procedure for the simultaneous determination of lipid and protein in biomembranes and other biological samples. Lipids 26: 77–80, 1991
Ostrowska J, Skrzydlewska E, Figaszewski Z: Isolation and analysis of phospholipids. Chem Anal 45: 613–618, 2000
Gill HS, Cross ML: Anticancer properties of bovine milk. Br J Nutr 84: 161–166, 2000
Podo F: Tumour phospholipid metabolism. NMR Biomed 12: 413–439, 1999
Needleman P, Turk J, Jakschik BA, Morrison AR: Arachidonic acid metabolism. Annu Rev Biochem. 55: 69–75, 1986
Glatz JFC, Borchers T, Spencer F, Van der Vusse J: Fatty acids in cell signalling: modulation by lipid binding proteins. Prostagland Leukot Essent Fatty Acids 52: 121–128, 1995
Spector AA, Yorek MA: Membrane lipid composition and cellular function. J Lipid Res 26: 1015–1035, 1985
Thornton WH Jr, MacDonald RS: Dietary fat quantity and composition induce changes in proliferation and membrane lipids in rat colon cells. Ann Nutr Metab 41: 260–8, 1997
Monteggia E, Colombo I, Guerra A, Berra B: Phospholipid distribution in murine mammary adenocarcinomas induced by activated neu oncogene. Cancer Detect Prev 24: 207–211, 2000
Dueck DA, Chan M, Tran K, Wong JT, Jay FT, Littman C, Stimpson R, Choy PC: The modulation of choline phosphoglyceride metabolism in human colon cancer. Mol Cell Biochem 20: 97–103, 1996
Aboagye EO, Bhujwalla ZM: Malignant transformation alters membrane choline phospholipid metabolism of human mammary epithelial cells. Cancer Res 59: 80–84, 1999
Reynier M, Sari H, d'Anglebermes M, Kye EA, Pasero L: Differences in lipid characteristics of undifferentiated and enterocytic-differentiated HT29 human colonic cells. Cancer Res 15: 1270–1277, 1991
Stampfer MR, Yaswen P: Culture system for study of human mammary epithelial cell proliferation, differentiation and transformation. Cancer Surv 18: 7–34, 1994
Koning GA, Gorter A, Scherphof GL, Kamps JA: Antiproliferative effect of immunoliposomes containing 5-fluorodeoxyuridinedipalmitate on colon cancer cells. Br J Cancer 80: 1718–1725, 1999
Schroeder F, Gardiner JM: Membrane lipids and enzymes of cultured high-and low-metastatic B16 melanoma variants. Cancer Res 44: 3262–3269, 1984
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Szachowicz-Petelska, B., Dobrzyńska, I., Figaszewski, Z. et al. Changes in physico-chemical properties of human large intestine tumour cells membrane. Mol Cell Biochem 238, 41–47 (2002). https://doi.org/10.1023/A:1019946718876
Issue Date:
DOI: https://doi.org/10.1023/A:1019946718876