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Development of Quantitative Enzyme Immunoassay and Radioimmunoassay of λ-Free Light Chains of Immunoglobulins

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Abstract

New quantitative techniques of radioimmunoassay (RIA) and enzyme immunoassay (EIA) were developed for determination of free light λ-chains of immunoglobulins (immunometric sandwich-like variants) in biological fluids using two types of monoclonal specific antibodies (MAB-1 and MAB-2) to different epitopes of the antigen molecule: MAB-1 were immobilized on a solid phase (polystyrene beads), whereas MAB-2 were labeled with iodine or with the enzyme. The test systems prepared can be used for determination of concentrations from 25 to 1000 ng/ml, are very sensitive (25 ng/ml), and the analysis time is 5 h. The two methods were compared, and their clinical and diagnostic validity was evaluated in patients with various diseases associated with disorders in the antibody synthesis by the immune system.

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Authors and Affiliations

  1. Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Minsk, 220141, Belarus

    N. V. Piven', A. V. Goncharik, G. A. Shkumatova & A. I. Kul'bakova

  2. Medical Center of the RF President's Administration, Moscow, 121359, Russia

    Yu. P. Reznikov

Authors
  1. N. V. Piven'
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  2. A. V. Goncharik
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  3. Yu. P. Reznikov
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  4. G. A. Shkumatova
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  5. A. I. Kul'bakova
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Piven', N.V., Goncharik, A.V., Reznikov, Y.P. et al. Development of Quantitative Enzyme Immunoassay and Radioimmunoassay of λ-Free Light Chains of Immunoglobulins. Applied Biochemistry and Microbiology 38, 493–499 (2002). https://doi.org/10.1023/A:1019937006292

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