Abstract
Residual dipolar couplings between 15N and 1H nuclear spins in HPr were used to determine the protein's orientation in a medium of bicelles, oriented by a magnetic field. In the case of wild-type HPr the protein's non-spherical shape can explain its orientation in this medium. In the case of the F48W mutant it was found that at least one other mechanism contributes to the observed orientation of the protein, to a degree that depends on the concentration of phosphate ions in the medium. We propose that the F48W mutant has a weak affinity towards the bicelle-surfaces that decreases with increasing phosphate concentrations. We used an order-parameter description to analyse this situation and to determine the axis of main order and the sign of the order parameter pertaining to this additional orientation mechanism.
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Al Hashimi, H.M., Valafar, H., Terrell, M., Zartler, E.R., Eidsness, M.K. and Prestegard, J.H. (2000) J. Magn. Reson., 143, 402–406.
Fischer, M.W., Losonczi, J.A., Weaver, J.L. and Prestegard, J.H. (1999) Biochemistry, 38, 9013–9022.
Fowler, C.A., Tian, F., Al Hashimi, H.M. and Prestegard, J.H. (2000) J. Mol. Biol., 304, 447–460.
King, V., Parker, M. and Howard, K.P. (2000) J. Magn Reson., 142, 177–182.
Landt, O., Grunert, H.P. and Hahn, U. (1990) Gene, 96, 125–128.
Lee, L.G., Britton, P., Parra, F., Boronat, A. and Kornberg, H. (1982) FEBS Lett., 149, 288–292.
Losonczi, J.A., Andrec, M., Fischer, M.W. and Prestegard, J.H. (1999) J. Magn Reson., 138, 334–342.
Mao, Q., Schmunk, T., Gerber, B. and Erni, B. (1995) J. Biol. Chem., 270, 18295–18300.
Ottiger, M. and Bax, A. (1998) J. Biomol. NMR, 12, 361–372.
Ridder, A.N.J.A., Morein, S., Stam, J.G., Kuhn, A., de Kruijff, B. and Killian, J.A. (2000) Biochemistry, 39, 6521–6528.
Robillard, G.T. and Blaauw, M. (1987) Biochemistry, 26, 5796– 5803.
Sambrook, C.R., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
Tjandra, N. and Bax, A. (1997) Science, 278, 1111–1114.
van Dijk, A.A., de Lange, L.C., Bachovchin, W.W. and Robillard, G.T. (1990) Biochemistry, 29, 8164–8171.
Zweckstetter, M. and Bax, A. (2000) J. Am. Chem. Soc., 120, 3791– 3792.
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van Lune, F., Manning, L., Dijkstra, K. et al. Order-parameter tensor description of HPr in a medium of oriented bicelles. J Biomol NMR 23, 169–179 (2002). https://doi.org/10.1023/A:1019877830921
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DOI: https://doi.org/10.1023/A:1019877830921