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Molecular analysis of de novo pyrimidine synthesis in solanaceous species

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Abstract

The de novo synthesis of pyrimidine nucleotides in plants has been analysed on a molecular level with special focus on cDNA cloning and structure analysis of all genes involved and their expression pattern during development. The exhaustive cloning of all cDNAs resulted from screening with heterologous cDNAs or by using complementation strategies with Escherichia coli mutants and subsequent enzyme activity measurements. Southern hybridization and comparison with the Arabidopsis genome reveals plant specific aspects and a simple genomic organization of pyrimidine synthesis in plants, which is superimposed by the postulated, complex subcellular compartmentalization. Northern hybridization evinces coordinated expression of all genes under developmental control during tobacco leaf growth.

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Author notes

  1. Rita Zrenner

    Present address: Botanisches Institut, Ruprecht-Karls-Universität Heidelberg, INF 360, 69120, Heidelberg, Germany

  2. Michael Schröder

    Present address: Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476 , Golm, Germany

Authors and Affiliations

  1. Botanisches Institut, Ruprecht-Karls-Universität Heidelberg, INF 360, 69120, Heidelberg, Germany

    Norbert Giermann & Tina Ritter

  2. Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476 , Golm, Germany

    Rita Zrenner

Authors
  1. Norbert Giermann
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  2. Michael Schröder
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  3. Tina Ritter
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  4. Rita Zrenner
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Giermann, N., Schröder, M., Ritter, T. et al. Molecular analysis of de novo pyrimidine synthesis in solanaceous species. Plant Mol Biol 50, 393–403 (2002). https://doi.org/10.1023/A:1019854531254

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