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Purification of glycyrrhizin from Glycyrrhiza uralensis Fisch with ethanol/phosphate aqueous two phase system

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Abstract

Phase diagrams of alcohol (ethanol or 2-propanol)/salt (phosphate or sulfate) aqueous two-phase systems were made. The system consisting of 60% (v/v) ethanol and 15% (w/v) phosphate was then used to separate glycyrrhizin from an extract of Glycyrrhiza uralensis Fisch and gave a 92% recovery of glycyrrhizin with 2.6-fold purification.

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Authors and Affiliations

  1. Department of Biochemical Engineering, Beijing University of Chemical Technology, Beijing, 100029, P.R. China

    Tan Tianwei, Huo Qing & Ling Qiang

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  1. Tan Tianwei
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  2. Huo Qing
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  3. Ling Qiang
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Tianwei, T., Qing, H. & Qiang, L. Purification of glycyrrhizin from Glycyrrhiza uralensis Fisch with ethanol/phosphate aqueous two phase system. Biotechnology Letters 24, 1417–1420 (2002). https://doi.org/10.1023/A:1019850531640

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