Abstract
Hydroxyapatite(HAp)/collagen nanocomposites were prepared by a coprecipitation method controlling the degree of cross-linkage between collagen molecules using glutaraldehyde. The precipitates filtered were dried in a freeze drier or naturally dried in the air at 25 °C. The naturally dried cakes had open channels of 5–15 μm in diameters, which were three-dimensionally and regularly developed over the whole samples, and showed a pretty good mechanical strength. The channels that were formed at spaces among the HAp/collagen particles, cross-linked one another, which had been filled up with water before its evaporation. The ordering state of the open channels depended on the degree of cross-linkage with glutaraldehyde; the optimal self-organized state was found when 30 molecules of glutaraldehyde were added per collagen molecule, though an excess amount of glutaraldehyde suppressed the appearance of the ordered state. From SEM and FT-IR measurements, it was indicated that the self-organization in the HAp/collagen nanocompsites continuously occurred during the drying process together with the removal of water and the increase of the density.
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References
J. S. Hanker and B. L. Giammara, Science 242 (1988) 885.
W. R. Walsh, M. Ohno and N. Guzelsu, J. Mater. Sci.: Mater. Med. 5 (1994) 72.
H. Ohashi, M. Therin, A. Meunier and P. Christel, ibid. 5 (1994) 237.
L. Gineste L, M. Gineste, X. Ranz, A. Ellefterion, A. Guilhem, N. Rouquet and P. Frayssinet, J. Biomed. Mater. Res. 48 (1999) 224.
P. Weiss, M. Lapkowski, R. Z. Legeros, J. M. Bouler, A. Jean and C. Daculsi, J. Mater. Sci.: Mater. Med. 8 (1997) 621.
S. Deb, M. Braden and W. Bonfield, ibid. 8 (1997) 829.
C. I. Vallo, P. E. Montemartini, M. A. Fanovich, J. M. Lopez and T. R. Cuadrao, J. Biomed. Mater. Res. 48 (1999) 150.
M. Kikuchi, Y. Suetsugu, J. Tanaka, S. Ito S, S. Ichinose, K. Shiniyama, Y. Hiraoka, Y. Mandai and S. Nakatani, Bioceramics 12 (1999) 393.
S. B. Cho, M. Kikuchi, Y. Suetsugu and J. Tanaka, Key Eng. Mater. 132–136 (1997) 802.
M. Kikuchi and J. Tanaka, J. Jap. Soc. Pow. Pow. Met. 45 (1998) 36.
M. Murata and J. Tanaka, Bioceramics 12 (1999) 429.
C. Du, F. Z. Cui, D. Zhu and K. D. Groot, J. Biomed. Mater. Res. 44 (1999) 407.
M. Ito, Y. Hidaka, M. Nakajima, H. Yagasaki and A. H. Kafrawy, ibid. 45 (1999) 204.
K. Sato, Y. Kumagai and J. Tanaka, Bioceramics 12 (1999) 137.
S. H. Rhee and J. Tanaka, ibid. 20 (1999) 2155.
L. H. H. Olde Damink, P. J. Dijkstra, M. J. A. Van Luyn, P. B. Van Wachem, P. Nieuwenhuis and J. Feijen, J. Mater. Sci.: Mater. Med. 8 (1997) 621.
J. M. Ruijgrok, J. R. D. Wijn and M. E. Boon, ibid. 5 (1994) 80.
F. Casagranda, J. A. Werkmeister and J. A. M. Ramshaw, ibid. 5 (1994) 332.
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Chang, M.C., Ikoma, T., Kikuchi, M. et al. The cross-linkage effect of hydroxyapatite/collagen nanocomposites on a self-organization phenomenon. Journal of Materials Science: Materials in Medicine 13, 993–997 (2002). https://doi.org/10.1023/A:1019825132610
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DOI: https://doi.org/10.1023/A:1019825132610