Seven strains with similar characteristics from the laboratory collection of yeasts isolated from forest substrates collected in the central part of European Russia corresponded to none of the known yeast species. Based on the study of their life cycle, physiological characteristics, and the nucleotide composition of their DNA and taking into account the data of PCR analysis with universal primers, the strains were ascribed to a new psychrophilic yeast species, Mrakia curviuscula sp. nov.
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Bab'eva, I.P., Golubev, V.I., Kartintsev, A.V., Gorin, ?S.E., and Zaslavskaya, P.L., Yeasts in Forest and Meadow Biogeocenoses, Vestn. Mosk. Univ., Ser. Biol. Pochvoved., 1973, no. 6, pp. 67–73.
Bab'eva, I.P., Yeasts in the Biogeocenoses of Different Natural Zones, Pochvennye organizmy kak komponenty biogeotsenoza (Soil Organisms as Components of Biogeocenoses), Moscow, 1984, pp. 131–141.
Bab'eva, I.P., Kartintseva, A.A., Maksimova, I.A., and Chernov, I.Yu., Yeasts in the Spruce Forests of the Central State Forest Biosphere Reserve, Vestn. Mosk. Univ., Ser. 17: Pochvoved., 1999, no. 4, pp. 45–49.
Bab'eva, I.P., Lisichkina, G.A., Maksimova, I.A., Reshetova, I.S., Terenina, E.E., and Chernov, I.Yu., A New Yeast Species, Candida anutae sp. nov., from the Fruiting Bodies of Agarics, Mikrobiologiya, 2000, vol. 69, no. 2, pp. 276–279.
Bab'eva, I.P. and Lisichkina, G.A., A New Species of Psychrophilic Basidiomycetous Yeasts Leucosporidium fasciculatum sp. nov., Mikrobiologiya, 2000, vol. 69, no. 6, pp.-801–804.
Maximova, I.A., Yeasts Biodiversity in Forest Ecosystems of European Russia, The rising power of Yeasts in Science and Industry, 10th Int. Symp. on Yeasts, 2000, p. ?138.
Kurtzman, C.P. and Fell, J.W., The Yeasts: A Taxonomic Study, New York: Elsevier, 1998.
Marmur, J.A., A Procedure for the Isolation of Deoxyribonucleic Acid from Microorganisms, J. Mol. Biol., 1961, vol. 3, pp. 208–214.
Owen, R.J., Hill, R.L., and Lapage, S.P., Determination of DNA Base Composition from Melting Profiles in Dilute Buffers, Biopolymers, 1969, vol. 7, pp. 503–516.
Danilevich, V.N. and Grishin, E.V., A New Approach to Isolation of Genomic DNA from Yeast and Fungi: Obtaining of DNA-Containing CM Envelopes and Their Direct Use in PCR, Bioorg. Khim., 2002, vol. 28, no. 2, pp. 156–167.
Bulat, S.A., Mironenko, N.V., and Zholkevich, Yu.G., The Genetic Structure of the Soil Population of the Fungus Fusarium oxysporum Schlechtend FR: The Molecular Reidentification of the Species and the Genetic Differentiation of Isolates by the Polymerase Chain Reaction Technique with Universal Primers (UP-PCR), Genetika, 1995, vol. 31, no. 3, pp. 315–323.
Maniatis, T., Fritsch, E.F., and Sambrook, J., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor: Cold Spring Harbor Lab., 1989.
Fell, J.W., Roejmans, H., and Boekhout, T., Cystofilobasidiales, a New Order of Basidiomycetous Yeasts, Int. J. Syst, Bacteriol., 1999, vol. 49, pp. 907–913.
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Bab'eva, I.P., Lisichkina, G.A., Reshetova, I.S. et al. Mrakia curviuscula sp. nov.: A New Psychrophilic Yeast from Forest Substrates. Microbiology 71, 449–454 (2002). https://doi.org/10.1023/A:1019801828620
- psychrophilic yeasts
- new yeast species