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Endotoxin Protection from Oxygen Toxicity: Effect on Pulmonary Neutrophils and L-Selectin

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Abstract

The mechanisms by which sublethal doses of endotoxin protect against hyperoxic lung injury are not completely understood. We hypothesized that endotoxin treatment would result in a decreased inflammatory response to hyperoxia and that this would be accompanied by activation of neutrophils (as evidenced by loss of L-selectin) in the peripheral circulation. Adult rats were injected with endotoxin 0.5 mg/kg prior to and 24 hr after onset of exposure to ≥98% O2. After 56 hr of hyperoxia, pulmonary neutrophils were lower in the O2/endotoxin group compared to O2 controls as measured by myeloperoxidase in lung homogenates and neutrophil counts in bronchoalveolar lavage fluid. Circulating neutrophils were also significantly lower in the O2/endotoxin group compared to O2 controls at 56 hr. Expression of the neutrophil adhesion molecule, L-selectin, was lower at 4 and 24 hr in the endotoxin-treated rats compared to O2 controls. There were no differences at 48 hr. Expression of CD18 rose significantly in the O2/endotoxin group after 4 hr, but thereafter did not differ from O2 controls. In summary, endotoxin protection from O2 toxicity was associated with reduced neutrophils in the lung and a loss of L-selectin from peripheral blood neutrophils.

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Authors and Affiliations

  1. Department of Pediatrics, University of Texas Medical Branch, Galveston, TX, 77555

    Susan E. Keeney, Mary J. Mathews, Karen E. Shattuck & Dara V. Dallas

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  1. Susan E. Keeney
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  2. Mary J. Mathews
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  4. Dara V. Dallas
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Keeney, S.E., Mathews, M.J., Shattuck, K.E. et al. Endotoxin Protection from Oxygen Toxicity: Effect on Pulmonary Neutrophils and L-Selectin. Inflammation 26, 243–252 (2002). https://doi.org/10.1023/A:1019770703047

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