Skip to main content
SpringerLink
Log in

Structural characterization of the trisaccharide chain from the skin mucus glycoprotein of the rainbow trout, Salmo gairdneri

Fish Physiology and Biochemistry volume 25, pages 11–17 (2001)Cite this article

Abstract

Skin mucus glycoprotein (RGP) was extracted from the skin mucus of rainbow trout, Salmo gairdneri. Alkaline borohydride treatment of RGP yielded a major oligosaccharide alditol NeuAcα2 to6GalNAc-ol and several minor oligosaccharide alditols. A trisaccharide alditol (OS-I) was purified by Sephadex G-25 gel filtration and high-performance liquid chromatography (HPLC). The amount of OS-I was estimated to be 7% in RGP from the amount of GalNAc-ol. Structural determination of the OS-I was examined. Structure of OS-I was identified to be NeuAcα2 to6 (GalNAcα1 to3) GalNAc-ol by α-N-acetylgalactosaminidase digestion, methylation analysis, and 1H-NMR spectroscopy.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

USD 39.95

Price includes VAT (Canada)

References

  • Capon C., Leroy Y., Wieruszeski J-M., Ricart G., Strecker G., Montreuil J. and Fournet B. 1989. Structures of O-glycosidically linked oligosaccharides isolated from human meconium glycoproteins. Eur. J. Biochem. 182: 139–152.

    Article  PubMed  CAS  Google Scholar 

  • Ciucanu I. and Kerek F. 1984. A simple and rapid method for the permethylation of carbohydrate. Carbohydr. Res. 131: 209–217.

    Article  CAS  Google Scholar 

  • Dua V. K., Dube V. E. and Bush C. A. 1984. The combination of normal-phase and reverse-phase high-pressure liquid chromatography with NMR for the isolation and characterization of oligosaccharide alditols from ovarian cyst mucins. Biochim. Biophys. Acta 80: 29–40.

    Google Scholar 

  • Feeney J., Frenkiel T. A. and Hounsell E. F. 1986. Complete 1H-NMR assignments for two core-region oligosaccharides of human meconium glycoproteins, using 1D and 2D methods at 500 MHz. Carbohydr. Res. 152: 63–72.

    Article  PubMed  CAS  Google Scholar 

  • Harris G. and MacWilliam I.C. 1954. A dipping technique for revealing sugars on paper chromatograms. Chem. Ind., London, 249.

    Google Scholar 

  • Inoue S., Kanamori A., Kitajima K. and Inoue Y. 1988. KDNglycoprotein: A novel deaminated neuraminic acid-rich glycoprotein isolated from vitelline envelope of rainbow trout eggs. Biochem. Biophys. Res. Commun. 153: 172–176.

    Article  PubMed  CAS  Google Scholar 

  • Iwasaki M., Inoue S., Nadano D. and Inoue Y. 1987. Isolation and structure of a novel deaminated neuraminic acid containing oligosaccharide chain present in rainbow trout egg polysialoglycoprotein. Biochemistry 26: 1452–1457.

    Article  CAS  Google Scholar 

  • Kanamori A., Kitajima K., Inoue S. and Inoue Y. 1989. Isolation and characterization of deaminated neuraminic acid-rich glycoprotein (KDN-gp-OF) in the ovarian fluid of rainbow trout (Salmo gairdneri). Biochem. Biophys. Res. Commun. 164: 744–749.

    Article  PubMed  CAS  Google Scholar 

  • Kimura M., Hama Y., Sumi T., Asakawa M., Narasinga Rao B.N., Horne A.P., Li S.-C., Li Y.-T. and Nakagawa H. 1994. Characterization of a deaminated neuraminic acid-containing glycoprotein from the skin mucus of the loach, Misgurnus anguillicaudatus. J. Biol. Chem. 269: 32138–32143.

    PubMed  CAS  Google Scholar 

  • Kochetkov N.K., Derevitskaya V.A., Likhosherstov L.M. and Medvedev S.A. 1974. The isolation and the structure of new glycopeptide from blood group substance. Biochem. Biophys. Res. Commun. 56: 311–316.

    Article  PubMed  CAS  Google Scholar 

  • Kurosaka A., Nakajima H., Funakoshi I., Matsuyama M., Nagaya T. and Yamashina I. 1983. Structures of the major oligosaccharides from a human rectal adenocarcinoma glycoprotein. J. Biol. Chem. 258: 11594–11598.

    PubMed  CAS  Google Scholar 

  • Mailsonrouge M.F. and Kobat E.A. 1976. Immunochemical studies on blood groups. Structures and immunochemical properties of oligosaccharides from two fractions of blood group substance from human ovarian cyst fluid differing in B, I, and activities and reactivity toward concanavalin A. Arch. Biochem. Biophys. 175: 90–113.

    Article  Google Scholar 

  • Nadano D., Iwasaki M., Endo S., Kitajima K., Inoue S. and Inoue Y. 1986. A naturally occurring deaminated neuraminic acid, 3-deoxy-D-glycero-D-galacto-nonulosonic acid (KDN). Its unique occurrence at the nonreducing ends of oligosialyl chains in polysialoglycoprotein of rainbow trout eggs. J. Biol. Chem. 261: 11550–11557.

    PubMed  CAS  Google Scholar 

  • Nakagawa H., Asakawa M. and Enomoto N. 1987. Purification and characterization of α-N-acetylgalactosaminidase from skipjack liver, J. Biochem. 101: 855–862.

    PubMed  CAS  Google Scholar 

  • Nakagawa H., Sumi T., Kimura M., Hama Y., Li S.-C. and Li Y.-T. 1995. Structures of KDN-containing sugar chains isolated from the skin mucus of the loach, Misgurnus anguillicaudatus. Glycoconjugate J. 12:516.

    Article  Google Scholar 

  • Oosten J.V. 1957. The skin and scales. In: The Physiology of Fishes. Vol. 1. pp. 207–244. Edited by M.E. Brown. Academic Press, New York.

    Google Scholar 

  • Pickering A.D. 1974. The distribution of mucous cells in the epidermis of the brown trout Salmo trutta and the char Salvelinus alpinus. J. Fish Biol. 6: 111–118.

    Article  CAS  Google Scholar 

  • Rose M.C. 1989. Characterization of human tracheobronchial mucin glycoproteins. Methods in Enzymol. 179: 3–17.

    Article  CAS  Google Scholar 

  • Rosen M.W. and Cornford N.E. 1971. Fluid friction of fish slimes. Nature 234: 49–51.

    Article  Google Scholar 

  • Savage A.V., Donoghue C.M., D'Arcy S.M., Koeleman C.A.M. and Eijnden D.H.V.D. 1990. Structure determination of five sialylated trisaccharides with core type 1, 3 or 5 isolated from bovine submaxillary mucin. Eur. J. Biochem. 192: 427–432.

    Article  PubMed  CAS  Google Scholar 

  • Strous G.J. and Dekker J. 1992. Mucin-type glycoproteins. Crit. Rev. Biochem. Mol. Biol. 27: 57–92.

    PubMed  CAS  Google Scholar 

  • Sumi T., Hama Y., Maruyama D., Asakawa M. and Nakagawa H. 1997. Purification and characterization of glycoprotein from the skin mucus of the rainbow trout, Salmo gairdneri. Biosci. Biotechnol. Biochem. 61: 675–683.

    Article  CAS  Google Scholar 

  • Sumi T., Hama Y., Nakagawa H. Maruyama D. and Asakawa M. 1999. Purification and characterization of eel skin mucus glycoprotein. Glycoconjugates J. 16: 115.

    Google Scholar 

  • Uchida Y., Tsukada Y. and Sugimori T. 1977. Distribution of neuraminidase in Arthrobacter and its purification by affinity chromatography. J. Biochem. 82: 1425–1433.

    PubMed  CAS  Google Scholar 

  • Vliegenthart J.F.G., Dorland L. and Halbeek H.V. 1983. Highresolution, 1H-nuclear magnetic resonance spectroscopy as a tool in the structural analysis of carbohydrates related to glycoproteins. Adv. Carbohydr. Chem. Biochem. 41: 209–374.

    Article  CAS  Google Scholar 

  • Vliegenthart J.F.G., Halbeek H.V. and Dorland L. 1981. The applicability of 500-MHz high-resolution 1H-NMR spectroscopy for the structure determination of carbohydrates derived from glycoproteins. Pure appl. Chem. 53: 45–77.

    CAS  Google Scholar 

  • Whitear M. 1984. Biology of the Integument 2. In: Vertebrates. pp. 8–38. Edited by J. Bereiter-Hahn, A.G. Matolsty and K. S. Richards. Springer-Verlag, Berlin.

    Google Scholar 

  • Wieruszeski J.-M., Michalski J.C., Montreuil J. Strecker G. Peter-Katalinic J., Egge H., Halbeek H.V., Mutsaers J.H.G.M. and Vliegenthart J.F.G. 1987. Structure of the monosialyl oligosaccharides derived from salivary gland mucin glycoproteins of the Chinese swiftlet (Genus Collocalia). Characterization of novel types of extended core structure, Gal beta(1----3)[GlcNAc beta(1----6)]GalNAc alpha(1----3) GalNAc(-ol), and of chain termination, [Gal alpha(1----4)]0-1[Gal beta(1----4)] 2GlcNAc beta(1----.). J. Biol. Chem. 262: 6650–6657.

    PubMed  CAS  Google Scholar 

Download references

Authors
  1. Toshihisa Sumi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yoichiro Hama
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hiroki Nakagawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Daisuke Maruyama
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Makio Asakawa
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Sumi, T., Hama, Y., Nakagawa, H. et al. Structural characterization of the trisaccharide chain from the skin mucus glycoprotein of the rainbow trout, Salmo gairdneri . Fish Physiology and Biochemistry 25, 11–17 (2001). https://doi.org/10.1023/A:1019763214180

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1019763214180

  • mucus
  • mucus glycoprotein
  • rainbow trout
  • sialoglycoprotein
  • trisaccharide

Search

Navigation