Abstract
Twelve strains of algicidal bacteria were isolated from the surfacewater of the pond Shinobazu and the moat Ote-bori (Tokyo, Japan). Nine of thesestrains were considered to be in the Pseudomonas group byanalyses of 16S rDNA sequences. The methanol extract ofPseudomonas sp. K44-1 showed marked antialgal activity bythe paper disk method. Harmane (1-methyl-β-carboline) was isolated fromtheethyl acetate extract of the whole culture broth ofPseudomonas sp. K44-1 by using silica gel columnchromatography and high performance liquid chromatography (HPLC). Harmaneshowedantagonistic activities against several cyanobacterial strains at aconcentration of 30 μg disk−1.
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References
Allen J.R.F. and Holmstedt B.R. 1980. The simple β-carboline alkaloids. Phytochemistry: 1573-1582.
Bohlendorf B., Forche E., Bedorf N., Gerth K., Irschik H., Jansen R. et al. 1996. Indole and quinoline derivatives as metabolites of tryptophan in myxobacteria. Liebigs Annalen 1: 49-53.
Carmichael W.W. 1992. A status report of planktonic cyanobacteria (Blue-green algae and their toxins.). #EPA/600/R-92/079. United States Environmental Protection Agency, Washington, DC, USA.
Cheng J. and Mitchelson K.R. 1997. Improved separation of six harmane alkaloids by high-performance capillary electrophoresis. J. Chromatogr. 761: 297-305.
Dakhama A., Noue J. and Lavoie M.C. 1993. Isolation and identi-fication of antialgal substances produced by Pseudomonas aeruginosa. J. appl. Phycol. 5: 297-306.
Harada K.-I. 1996. Chemistry and detection of microcystins. In: Watanabe M.F., Harada K.-I., Carmichael W.W. and Fujiki H. (eds), Toxic Microcystis. CRC Press, pp. 103-148.
Kikuchi T., Miura T., Harimaya K., Yano H., Arimoto T., Masada Y. et al. 1973. Odorous metabolites of blue-green alga: Schizothrix muelleri Nägeli collected in the southern basin of Lake Biwa. Identification of geosmin. Chem. Pharm Bull. 21: 2342- 2343.
Kimura M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol. 16: 111-120.
Mitsutani A., Uchida A. and Ishida Y. 1987. Occurrence of bluegreen algae and algal lytic bacteria in Lake Biwa. Bull. Jap.Soc. Microbiol. Ecol. 2: 21-28.
Neefs J.M., Van de Peer Y., Hendriks L. and Watcher R. 1990. Compilation of small ribosomal subunit RNA sequences. Nucleic Acids Res. 18: 2237-2317.
Palmer C.M. and Maloney T.E. 1955. Preliminary screening for potential algicides. Ohio J. Sci. 55: 1-8.
Rashidan K.K. and Bird D.F. 2000. Role of predatory bacteria in the termination of a cyanobacterial bloom. Microb. Ecol. 16: 97-105.
Reim R.L., Shane M.S. and Cannon R.E. 1974. The characterization of a Bacillus capable of blue-green bactericidal activity. Can. J. Microbiol. 20: 981-986.
Safferman R.S. and Morris M.E. 1962. Evaluation of natural products for algicidal properties. Appl. Microbiol. 10: 289-292.
Saitou N. and Nei M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4: 406-425.
Takeuchi T., Ogawa K., Iinuma H., Suda H., Ukita K., Nagamatsu T. et al. 1973. Monoamine oxidase inhibitors isolated from fermented broths. J. Antibiot. 16: 162-177.
Takase S. and Murakami H. 1966. Studies on the fluorescence of Saké. I. Fluorescence spectrum of Saké and identification of harmane. Agr. Biol. Chem. 30: 869-876.
Thompson J.D., Higgin D.G. and Gibson T.J. 1994. CLUSTALW: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.
Tsuchiya Y., Watanabe M.F. and Watanabe M. 1992. Volatile organic sulfur compounds associated with blue-green algae from inland waters of Japan. Wat. Sci. Technol. 25: 123-130.
Watanabe M.M., Kawauchi M., Hiroki M. and Kasai F. 2000. NIES-Collection, List of Strains, Microalgae and Protozoa; Microbial Culture Collection. National Institute for Environmental Science, Japan, 30-32.
Yamamoto Y. 1978. Detection of algal lysing biological agents in lakes by the soft-agar overlayer technique. Jap. J. Limnol. 39: 9-14.
Yamamoto Y., Kouchiwa T., Hodoki Y., Hotta K., Uchida H. and Harada K.-I. 1998. Distribution and identification of actinomycetes lysing cyanobacteria in a eutrophic lake. J. Appl. Phycol. 10: 391-397.
Yamamoto Y., Niizuma S., Kuroda N. and Sakamoto M. 1993. Occurrence of heterotrophic bacteria causing lysis of cyanobacteria in a eutrophic lake. Jap. J. Phycol. 41: 215-220.
Yoshikawa K., Adachi K., Nishijima M., Takadera T., Tamaki S., Harada K.-I. et al. 2000. β-Cyanoalanine production by marine bacteria on cyanide-free medium and its specific inhibitory activity toward cyanobacteria. Appl. Environ. Microbiol. 66: 718-722.
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Kodani, S., Imoto, A., Mitsutani, A. et al. Isolation and identification of the antialgal compound, harmane (1-methyl-β-carboline), produced by the algicidal bacterium, Pseudomonas sp. K44-1. Journal of Applied Phycology 14, 109–114 (2002). https://doi.org/10.1023/A:1019533414018
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DOI: https://doi.org/10.1023/A:1019533414018