Abstract
A useful approach for constructing dose–response relationships and for studying the underlying mechanisms by which a xenobiotic agent enhances airway reactivity is to measure the response of an isolated airway following ex vivo exposure to a pollutant. We have in this way determined the dose–response relationship between ex vivo exposure to pollutants such as nitrogen dioxide (NO2), the aldehyde acrolein, and ozone (O3) and the reactivity to agonists in human isolated bronchial smooth muscle. We have also investigated the underlying alteration in the cellular mechanisms of airway smooth-muscle contraction induced by such exposure and found that it is related to alteration in calcium signaling at the site of the airway smooth-muscle cell. Finally, although there is epidemiological evidence that an increase in allergic diseases such as asthma may be linked to air pollution, there are few experimental data to address this issue. The final aim of this study was therefore to investigate the interaction between passive sensitization and exposure to pollutants in human isolated airways. We have examined (i) the effect of a pre-exposure to pollutants on the contraction of sensitized bronchi in response to a specific antigen and (ii) the effect of passive sensitization on the contraction in response to nonspecific agonists in bronchi pre-exposed to pollutants. The results indicate a combined effect of immunological sensitization and exposure to pollutants; that is, passive sensitization and exposure to pollutants act in a synergistic manner on human bronchial smooth-muscle reactivity in response to both specific antigens and nonspecific agonists.
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References
American Thoracic Society. Health effects of outdoor air pollution. Part 1. Am J Respir Crit Care Med. 1996a; 153: 3–50.
American Thoracic Society. Health effects of outdoor air pollution. Part 2. Am J Respir Crit Care Med. 1996b; 153: 477–98.
Ben-Jebria A, Marthan R, Savineau JP. Effect of in vitro nitrogen dioxide exposure on human bronchial smooth muscle response. Am Rev Respir Dis. 1992; 146: 378–82.
Ben-Jebria A, Marthan R, Rossetti M, Savineau JP. Effect of passive sensitization on the mechanical activity of human isolated bronchial smooth muscle induced by substance P, neurokinin A and VIP. Br J Pharmacol. 1993a; 109: 131–6.
Ben-Jebria A, Marthan R, Rossetti M, Savineau JP, Ultman JS. Effect of in vitro exposure to acrolein on carbachol responses in rat trachealis muscle. Respir Physiol. 1993b; 93: 111–23.
Ben-Jebria A, Marthan R, Rossetti M, Savineau JP, Ultman JS. Human bronchial smooth muscle responsiveness after in vitro exposure to acrolein. Am J Respir Crit Care Med. 1994; 149: 382–6.
Berger P, Walls AF, Marthan R, Tunon-de-Lara JM. Immunologlobulin E-induced passive sensitization of human airways. Am J Respir Crit Care Med. 1998; 157: 610–6.
Bignon J. Effets sur la sante¨ respiratoire de l'homme du dioxyde d'azote, de l'ozone et autres polluants oxydants de l'atmo-sphe© re. Pollution Atmosphe¨ rique. 1990; 128: 431–40.
Black JL, Marthan R, Armour CL, Johnson PR. Sensitization alters contractile responses and calcium in£ux in human airway smooth muscle. J Allergy Clin Immunol. 1989; 84: 440–7.
Dab W, Medina S, Quenel P. Short term respiratory health effects of ambient air pollution: results of the APHEA project in Paris. J Epidemiol Community Health. 1996; 50(supplement 1): S42–6.
Del¢no RJ, Coate BD, Zeiger RS, Seltzer JM, Street DH, Koutrakis P. Daily asthma severity in relation to personal ozone exposure and outdoor fungal spores. Am J Respir Crit Care Med. 1996; 154: 633–41.
Devalia JL, Rusznak C, Wang J, Davies RJ. Pollution-allergen interactions: challenge studies in man. Eur Respir Rev. 1998; 8: 175–8.
Gilmour MI. Interaction of air pollutants and pulmonary allergic responses in experimental animals. Toxicology. 1995; 105: 335–42.
Guibert C, Marthan R, Savineau JP. Oscillatory Clcurrent induced by angiotensin II in rat pulmonary arterial myocytes: Ca2+ dependence and physiological implication. Cell Calcium. 1997; 21: 421–9.
Hyvelin JM, Roux E, Prevost MC, Savineau JP, Marthan R. Cellular mechanisms of acrolein-induced alteration in calcium signaling in airway smooth muscle. Toxicol Appl Pharmacol. 2000; 164: 176–83.
Hyvelin JM, Savineau JP, Marthan R. Selected contribution: effect of the aldehyde acrolein on acetylcholine-induced membrane current in airway smooth muscle cells. J Appl Physiol. 2001; 90: 750–4.
Jorres R, Nowak D, Magnussen H. The effect of ozone exposure on allergen responsiveness in subjects with asthma or rhinitis. Am J Respir Crit Care Med. 1996; 153: 56–64.
Koenig JQ. Effect of ozone on respiratory responses in subjects with asthma. Environ Health Perspect. 1995; 103(supplement 2): 103–5.
Marthan R, Crevel H, Guenard H, Savineau JP. Responsiveness to histamine in human sensitized airway smooth muscle. Respir Physiol. 1992; 90: 239–50.
Ouedraogo N, Roux E, Forestier F, Rossetti M, Savineau JP, Marthan R. Effects of intravenous anesthetics on normal and passively sensitized human isolated airway smooth muscle. Anesthesiology. 1998; 88: 317–26.
Rossetti M, Savineau JP, Crevel H, Marthan R. Role of protein kinase C in nonsensitized and passively sensitized human isolated bronchial smooth muscle. Am J Physiol. 1995; 268: L966–71.
Roux E, Guibert C, Crevel H, Savineau JP, Marthan R. Human and rat airway smooth muscle responsiveness after ozone exposure in vitro. Am J Physiol. 1996; 271: L631–6.
Roux E, Guibert C, Savineau JP, Marthan R. [Ca ]i oscillations induced by muscarinic stimulation in airway smooth muscle cells: receptor subtypes and correlation with the mechanical activity. Br J Pharmacol. 1997; 120: 1294–301.
Roux E, Hyvelin JM, Savineau JP, Marthan R. Calcium signaling in airway smooth muscle cells is altered by in vitro exposure to the aldehyde acrolein. Am J Respir Cell Mol Biol. 1998a; 19: 437–44.
Roux E, Molimard M, Savineau JP, Marthan R. Muscarinic stimulation of airway smooth muscle cells. Gen Pharmacol. 1998b; 31: 349–56.
Roux E, Hyvelin JM, Savineau JP, Marthan R. Human isolated airway contraction: interaction between air pollutants and passive sensitization. Am J Respir Crit Care Med. 1999; 160: 439–45.
Savineau JP, Marthan R. Cytosolic calcium oscillations in smooth muscle cells. News Physiol Sci. 2000; 15: 50–5.
Socie¨ te¨ Franc° aise de Sante¨ Publique. La pollution atmosphe¨ rique d'origine automobile et la sante¨ publique. Bilan de 15 ans de recherche internationale. In: Collection Sante¨ et Socie¨ te¨ no. 4. 1996: 1–251.
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Roux, E., Ouedraogo, N., Hyvelin, JM. et al. In vitro effect of air pollutants on human bronchi. Cell Biol Toxicol 18, 289–299 (2002). https://doi.org/10.1023/A:1019514830173
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DOI: https://doi.org/10.1023/A:1019514830173