Somatic Electrical Nerve Stimulation Regulates the Motility of Sphincter of Oddi in Rabbits and Cats (Evidence for a Somatovisceral Reflex Mediated by Cholecystokinin)

Abstract

Cholecystokinin (CCK) plays an important role inregulating the biliary motility in herbivorous andcarnivorous animals. Little is known about how themotility of the sphincter of Oddi (SO) is regulated through a somatic stimulation. It was our aimto test the hypothesis that somatic electrical nervestimulation (SENS) affects SO motility in animals withdifferent types of SO through CCK-related mechanisms. The activity of SO in anesthetized rabbits andcats was measured by using a continuously perfusedopen-tip manometric method. SENS was brought about byapplying an electric current (2/15 Hz alternatively, 20 min) to two needles positioned near spinalnerves in the 6th and 7th intercostal space in the rightmidclavicular line. The SO motility before and X minafter the start of SENS, designated as pre-SENS and SENS-X respectively, were recorded andsaved in a computer equipped with off-line analysissoftware. The SO activity in rabbits, in terms of phasiccontraction pressure and duration of summation peak during SENS were significantly higher than thatbefore SENS. The phasic contraction pressure ofpre-SENS, SENS-10, and SENS-16 were 6.83 ± 0.39mm Hg, 9.23 ± 0.83 mm Hg and 10.46 ± 0.81mm Hg, respectively (P < 0.03, N = 13). The duration ofsummation peak in pre-SENS, SENS-10, and SENS-16 were7.26 ± 0.41 sec, 10.22 ± 0.46 sec, and13.49 ± 2.31 sec, respectively (P < 0.05, N =13). The SENS-induced SO hyperactivity was not inhibited bypretreatment with atropine, propranolol, phentolamine,or naloxone, but was blocked by pretreatment with theCCK receptor antagonist, proglumide, and by injection of anti-CCK-8 antibody during SENS in adose-dependent manner. In contrast, SENS induced aninhibitory SO response in cats. However, in bothcircumstances, an obvious elevation of plasma CCK leveldetermined by radioimmunoassay was noted after SENS. Weconclude that SENS causes secretion of CCK, which inturn affects biliary tract motility in animals withdifferent types of SO. This provides an easilyapplicable method for those patients who have hyperactiveSO function.

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Chiu, JH., Kuo, YL., Lui, WY. et al. Somatic Electrical Nerve Stimulation Regulates the Motility of Sphincter of Oddi in Rabbits and Cats (Evidence for a Somatovisceral Reflex Mediated by Cholecystokinin). Dig Dis Sci 44, 1759–1767 (1999). https://doi.org/10.1023/A:1018870017774

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  • CHOLECYSTOKININ
  • SOMATIC NERVE STIMULATION
  • SPHINCTER OF ODDI
  • ELECTROACUPUNCTURE
  • MOTILITY