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Increased Expression of Transforming Growth Factor-β1 During Gastric Ulcer Healing in Rats

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Abstract

This study was done to investigate theexpression and localization of transforming growthfactor-β1 (TGF-β1) inthe gastric ulcerated tissues produced by acetic-acidduring the healing process, by northern blot analysis and immunohistochemicaltechnique. Ulcerated TGF-β1 mRNA levelswere significantly increased from days 3 to 18, in asimilar manner to extracellular matrix proteins, andreturned to control levels at the scarred phase.Immunoreactive TGF-β1 was localized inepithelial cells beneath proliferative zone in intacttissues. 1 In ulcerated tissues, TGF-β1was localized in macrophages in the ulcer bed and in fibroblasts ormyofibroblasts in the granulation tissues. Treatmentwith prostaglandin E1 (PGE1)further stimulated ulcerated TGF-β1expression, being associated with the acceleration of gastric ulcer healing, while treatment withindomethacin reduced TGF-β1 expression,being accompanied by the delayed ulcer healing. Thecombination of PGE1 and indomethacin reversedthe indomethacin-induced decrease in ulcerated TGF-β1.Thus, TGF-β1 may be implicated in theacceleration of gastric ulcer healing.

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Authors
  1. Kazunari Tominaga
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  2. Tetsuo Arakawa
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  3. Shokei Kim
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  4. Hiroshi Iwao
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  5. Kenzo Kobayashi
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Tominaga, K., Arakawa, T., Kim, S. et al. Increased Expression of Transforming Growth Factor-β1 During Gastric Ulcer Healing in Rats. Dig Dis Sci 42, 616–625 (1997). https://doi.org/10.1023/A:1018867630686

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  • DOI: https://doi.org/10.1023/A:1018867630686

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