Abstract
Purpose. The purpose of this study was to design a well-characterized liposomal carrier system for multivalent antigen presentation in order to activate T cells.
Methods. MHC class II molecules were loaded with peptide and subsequently reconstituted into liposomes. A FACS assay was developed to monitor peptide loading and MHC class II incorporation in the liposomes. For in vitro testing of the resulting MHC class II/peptide liposomes, a T cell hybridoma assay was employed.
Results. The FACS assay provided a qualitative means to visualize the amount of incorporated MHC class II and peptide molecules that were oriented in the appropriate way for antigen presentation to the T cells. Interestingly, when MHC class II molecules were loaded with the appropriate peptide prior to liposome incorporation, such liposomes were fully capable of inducing IL-2 production of a T cell hybridoma.
Conclusions. This is the first article showing that MHC class II/peptide liposomes can serve as 'artificial antigen presenting cells' for activation of a CD4+ T cell hybridoma. As compared to soluble MHC class II/ peptide complexes, the multivalency of liposomal complexes may be an important advantage when studying possible applications in immunotherapy.
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van Rensen, A.J.M.L., Wauben, M.H.M., Grosfeld-Stulemeyer, M.C. et al. Liposomes with Incorporated MHC Class Il/Peptide Complexes as Antigen Presenting Vesicles for Specific T Cell Activation. Pharm Res 16, 198–204 (1999). https://doi.org/10.1023/A:1018864005620
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DOI: https://doi.org/10.1023/A:1018864005620