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Identification of H. pylori in Saliva by a Nested PCR Assay Derived from a Newly Cloned DNA Probe

Digestive Diseases and Sciences volume 43, pages 1211–1218 (1998)Cite this article

Abstract

A novel probe was developed from genomic DNA ofHelicobacter pylori ATCC type strain 43629. Ithybridized with all 73 H. pylori clinical isolatestested but not with any of 183 non-H. pylori DNAs in dot blot hybridization. Typing tests revealed 41different HaeIII-digestion patterns from 57 H. pyloristrains tested. Based on the sequence of the probe, anested PCR was developed that detected as little as 2 fg of H. pylori DNA or approximatelyequivalent to one cell. No PCR products were amplifiedfrom any of 21 non-H. pylori strains tested. Using thisnested PCR, H. pylori DNA was detected in 33 of 45 (73%) saliva samples collected from patientswith gastric H. pylori infection. These data suggestthat the probe is useful for typing H. pylori and thatthe nested PCR is a valuable tool for detecting H. pylori DNA in saliva.

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Authors
  1. Chuancang Jiang
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  2. Chuanfu Li
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  3. Tuanzhu Ha
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  4. Donald A. Ferguson
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  5. David S. Chi
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  6. John J. Laffan
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  7. Eapen Thomas
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Jiang, C., Li, C., Ha, T. et al. Identification of H. pylori in Saliva by a Nested PCR Assay Derived from a Newly Cloned DNA Probe. Dig Dis Sci 43, 1211–1218 (1998). https://doi.org/10.1023/A:1018847522200

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  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1018847522200

  • HELICOBACTER PYLORI
  • DNA PROBE
  • NESTED PCR
  • TYPING
  • SALIVA