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Intestinal Expression of Human Heat Shock Protein 90 in Patients with Crohn's Disease and Ulcerative Colitis

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Abstract

Heat shock proteins are induced by severalstress factors and are potential antigens in autoimmunedisorders. Expression of heat shock protein 90 (HSP 90)was investigated in patients with inflammatory bowel disease and normal controls. We combinedwestern blot analysis with laser densitometry forquantitation. Localization of HSP 90 was investigated byimmunohistochemistry. Western blots showed a significant mucosal expression of HSP 90, which wascomparable in patients and controls. There was also nodifference between normal and inflamed mucosa ininflammatory bowel disease. In immunohistochemicalstaining studies, HSP 90 was detected in epithelialcells, mononuclear cells, giant cells, nerve cells, andendothelial cells of small vessels. There was nodifference in the intensity of staining or localization in patients with inflammatory bowel diseasecompared to controls. These findings render a potentialprotective or immunogenic function of HSP 90 ininflammatory bowel disease unlikely.

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REFERENCES

  1. Monsen U, Brostrom O, Nordenwall B, Sörstad J, Helle rs G: Prevalence of in¯ ammatory bowel disease among re latives of patients with ulcerative colitis. Scand J Gastroenterol 22:214 - 218, 1989

    Google Scholar 

  2. Hugot JP, Laurent-Puig P, Gower-Rousseau C, Olson JM, Lee JC, Beaugerie L, Naom I, Dupas JL, Van Gossum A, Groupe d'Etude The rapeutique des Affections Inammatoire s Digestives, Orholm M, Bonaiti-Pellie C, We issenbach J, Mathew CG, Lennard-Jone s JE, Cortot A, Colombel JF, Thomas G: Mapping of a susceptibility locus for Crohn's disease on chromosome 16. Nature 379:821- 823, 1996

    Google Scholar 

  3. Ibbotson JP, Pe ase PE, Allan RN: Serological studies in Crohn's disease. Eur J Clin Microbiol 6:286 - 290, 1987

    Google Scholar 

  4. Sanderson JD, Moss MT, Tizard MLV, Hermon-Taylor J: Mycobacterium paratube rculosis DNA in Crohn's disease tissue. Gut 33:890 - 896, 1992

    Google Scholar 

  5. Wakefield AJ, Pittilo RM, Sim R, Cosby SL, Stephenson JR, Dhillon AP, Pounder RE: Evidence of persistent measles virus infection in Crohn' s disease. J Med Virol 39:345- 353, 1993

    Google Scholar 

  6. Targan SR, Lande rs CJ, Cobb L, Mac Dermott RP, Vidrich A: Perinuclear anti-neutrophil cytoplasmatic antibodies are spontaneously produced by mucosal B ce lls of ulcerative colitis patients. J Immunol 155:3262- 3267, 1995

    Google Scholar 

  7. Podolsky DK: Inflammatory bowel disease (first of two parts). N Engl J Med 325:928 - 937, 1991

    Google Scholar 

  8. Ellis RJ: The general concept of molecular chaperone s. Phil Trans R Soc London Ser B 339:257- 262, 1993

    Google Scholar 

  9. Pelham HRB: Speculations on the functions of the major heat shock and glucose-regulated proteins. Cell 46:959 - 961, 1986

    Google Scholar 

  10. Musch MW, Ciancio MJ, Sarge K, Chang EB: Induction of heat shock protein 70 protects intestinal epithelial IEC-18 ce lls from oxidant and thermal injury. Am J Physiol 270(Ce ll Physiol 39):C429 - C436, 1996

    Google Scholar 

  11. Deitch EA, Beck SC, Cruz NC, De Maio AA: Induction of heat shock gene expression in colonic epithel cells after incubation with Escherichia colior endotoxin. Crit Care Med 23:1371- 1376, 1995

    Google Scholar 

  12. Kaufmann SHE, Schoel B: He at shock proteins as antigens in immunity against infection and se lf. InThe Biology of Heat Shock Proteins and Molecular Chaperone s. RI Morimoto, A Tissieres, C Georgopoulos (eds). Cold Spring Harbor, New York, Cold Spring Harbor Laboratory Pre ss, 1994, pp 495- 531

    Google Scholar 

  13. Cohen IR: Autoimmunity to chaperonins in the pathogenesis of arthritis and diabete s. Annu Rev Immunol 9:567- 589, 1991

    Google Scholar 

  14. Van Eden W, Thole JER, van der Zee R, Noordzij A, van Embden JDA, Hensen EJ, Cohen IR: Cloning of the mycobacte rial epitope recognized by T lymphocytes in adjuvant arthritis. Nature 331:171- 173, 1988

    Google Scholar 

  15. Bohen SP, Yamamoto KR: Modulation of steroid re ceptor signal transduction by heat shock proteins. InThe Biology of He at Shock Prote ins and Molecular Chape rones. RI Morimoto, A Tissieres, C Georgopoulos (eds). Cold Spring Harbor, New York, Cold Spring Harbor Laboratory Press, 1994, pp 313- 334

    Google Scholar 

  16. Pratt WB: The role of heat shock proteins in regulating the function, folding and trafficking of the glucocorticoid receptor. J Biol Chem 268:21455- 21458, 1993

    Google Scholar 

  17. Fe rris DK, Harel-Be llan A, Morimoto RI, We lch WJ, Farrar WL: Mitogen and lymphokine stimulation of heat shock proteins in T lymphocyte s. Proc Natl Acad Sci USA 85:3850 - 3854, 1988

    Google Scholar 

  18. Pee termans WE, D'Hae ns GR, Ceuppens JL, Rutgeerts P, Geboe s K: Mucosal expre ssion by B7-positive ce lls of the 60-kilodalton heat-shock protein in inammatory bowel disease. Gastroenterology 108:75- 82, 1995

    Google Scholar 

  19. Mary JY, Modigliani R: De velopment and validation of an endoscopic index of the severity for Crohn's disease: A prospective multicentre study [Groupe d'Etudes Therape utiques des affection inammatoires du tube digestif (GETAID) ]. Gut 30:983- 989, 1989

    Google Scholar 

  20. Rachmile witz D: Coated mesalazine (5-aminosalicylic acids) versus sulphasalazine in the treatment of active ulcerative colitis: a randomized trial. BMJ 298:82- 86, 1989

    Google Scholar 

  21. Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartne r FH, Provenzano MD, Fujimoto EK, Goeke NM, Olson BJ, Klenk DC: Measureme nt of protein using bicinchonicic acid. Anal Biochem 150:76 - 85, 1985

    Google Scholar 

  22. Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:6080 - 6085, 1970

    Google Scholar 

  23. Zhao Y, Levin RM, Monson FC, Chacko S: Expression of constitutive heat shock protein-70 in normal (non-stressed) rabbit urinary bladder tissue. Mol Cell Biochem 121:13- 19, 1993

    Google Scholar 

  24. Shi SR, Chaiwun B, Young I, Cote RJ, Taylor CL: Antigen retrieval technique utilizing citrate buffer or urea solution for immunohistochemical demonstration of androgen rece ptor in formalin-fixed paraffin se ctions. J Histochem Cytochem 41:1599 - 1604, 1993

    Google Scholar 

  25. Cordell JL, Falini B, Erber WN, Ghosh AK, Abdulaziz Z, Mac Donald S, Pulford KAF, Stein H, Mason DY: Immune nzymatic labeling of monoclonal antibodies using immune complexe s of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J Histochem Cytochem 32:219 - 229, 1984

    Google Scholar 

  26. Minota S, Came ron B, Welch WJ, Winfie ld JB: Autoantibodies to the constitutive 73-KD member of the hsp 70 family of heat shock proteins in systemic lupus erythematosus. J Exp Med 168:1475- 1480, 1988

    Google Scholar 

  27. Arora SK, Singh G, Seghal S: Comparative evaluation of anti-heat shock protein antibodies in SLE and healthy controls. Scand J Rheumatol 24:160 - 163, 1995

    Google Scholar 

  28. Hrycaj P, Wurm K, Mennet P, Müller W: Antibodies to heat shock proteins in patients with pulmonary sarcoidosis. Sarcoidosis 12:124 - 130, 1995

    Google Scholar 

  29. De Graeff-Meeder ER, Voorhorst M, Van Eden W, Schuurman HJ, Huber D, Barkley D, Maini RN, Kuis W, Rijkers GT, Ze ge rs BJM: Antibodies to the mycobacte rial 65-kd heat shock protein are reactive with synovial tissue adjuvant arthritic rats and patients with rheumatoid arthritis and osteoarthritis. Am J Pathol 137:1013- 1017, 1990

    Google Scholar 

  30. Patte rson DSP, Allen WM: Chronic mycobacte rial enteritis in ruminants as a model for Crohn's disease: Proc R Soc Med 65:998 - 1001, 1972

    Google Scholar 

  31. Markesich DC, Sawai ET, Butel JS, Graham DY: Investigations on etiology of Crohn's disease: Humoral immune re sponse to stress (heat shock) proteins. Dig Dis Sci 36:454 - 460, 1991

    Google Scholar 

  32. Kaufmann SHE: He at shock proteins and the immune re sponse. Immunol Today 11:129 - 135, 1990

    Google Scholar 

  33. Wand-Würtemberge r A, Schoel B, Ivanyi J, Kaufmann SHE: Surface expression by mononuclear phagocyte s of an epitope shared with mycobacterial heat shock protein 60. Eur J Immunol 21:1089 - 1092, 1991

    Google Scholar 

  34. Pirze r U, Schönhaar A, Fleische r B, Hermann E, Meyer zum Büschenfelde KH: Reactivity of infiltrating T lymphocyte s with microbial antigens in Crohn's disease. Lance t 338:1238 - 1239, 1991

    Google Scholar 

  35. Riabowol KT, Mizzen LA, Welch W: He at shock is lethal to fibroblasts microinjected with antibodies against hsp 70. Science 242:433- 436, 1988

    Google Scholar 

  36. Heufelder AE, Goe llner JR, Wenzel BE, Bahn RS: Immunohistochemical dete ction and localization of a 72 kilodalton heat shock protein in autoimmune thyroid disease. J Clin Endocrinol Metab 74:724 - 731, 1992

    Google Scholar 

  37. Winrow VR, Mojdehi GM, Ryder SD, Rhodes JM, Blake DR, Rampton DS: Stress proteins in colorectal mucosa. Enhanced expression in ulcerative colitis. Dig Dis Sci 38:1994 - 2000, 1993

    Google Scholar 

  38. Minota S, Koyasu S, Yahara I, Winfie ld J: Autoantibodies to the heat shock protein hsp 90 in systemic lupus erythematosus. J Clin Invest 81:106 - 109, 1988

    Google Scholar 

  39. Norton PM, Isenberg DA, Latchmann DS: Elevated levels of the 90 kd heat shock protein in a proportion of SLE patients with active disease. J Autoimmun 2:187- 195, 1989

    Google Scholar 

  40. Zhao Y, Chacko S, Levin RM: Expre ssion of stress proteins (HSP 70 and HSP 90) in the rabbit urinary bladder subjected to partial outlet obstruction. Mol Cell Biochem 130:49 - 55, 1994

    Google Scholar 

  41. Baca-Estrada ME, Gupta RS, Stead RH, Croitoru K: Intestinal expression and ce llular immune response s to human heatshock protein 60 in Crohn's disease. Dig Dis Sci 39:498 - 506, 1994

    Google Scholar 

  42. Parsell DA, Lindquist S: The function of heat shock proteins in stress tolerance: Degradation and reactivation of damaged proteins. Annu Rev Gene t 27:437- 496, 1993

    Google Scholar 

  43. Yonehara M, Minami Y, Kawata Y, Nagai J, Yahara I: Heat induced chaperone activity of HSP 90. J Biol Chem 271:2641- 2645, 1996

    Google Scholar 

  44. Gass P, Schroder H, Prior P, Kiessling M: Constitutive expression of heat shock protein 90 (HSP 90) in neurons of the rat brain. Neurosci Lett 182:188 - 192, 1994

    Google Scholar 

  45. Itoh H, Tashima Y, Eishi Y, Okeda R: Localisation of HSP 90 in rat brain. Int J Biochem 25:93- 99, 1993

    Google Scholar 

  46. Quaraishi H, Brown I: Expre ssion of heat shock protein 90 (hsp 90) in neuronal and nonneuronal tissues of the control and hype rthermic rabbit. Exp Cell Re s 219:358 - 363, 1995

    Google Scholar 

  47. Vamvakopoulos NO: Tissue-spe cifi c expression of heat shock proteins 70 and 90: Potential implication for differential sensitivity of tissues to glucocorticoids. Mol Cell Endocrinol 98:49 - 54, 1993

    Google Scholar 

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Authors
  1. Maren Stah
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  2. Diether Ludwig
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  3. Klaus Fellermann
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  4. Eduard F. Stange
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Stah, M., Ludwig, D., Fellermann, K. et al. Intestinal Expression of Human Heat Shock Protein 90 in Patients with Crohn's Disease and Ulcerative Colitis. Dig Dis Sci 43, 1079–1087 (1998). https://doi.org/10.1023/A:1018847205420

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  • DOI: https://doi.org/10.1023/A:1018847205420

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