Abstract
We analyzed the TcR Vβ gene usage beforeand after vaccination with the hepatitis B vaccine sincechanges in the TcR Vβ gene families would beconsidered to provide preliminary evidence of amechanism to prevent HBV infection. Six healthy adultvolunteers received immunizations. TcR Vβ usage,T-cell proliferation, and HLA class II alleles wereexamined in peripheral blood mononuclear cells (PBMC) both before and after vaccination. Furthermore,TcR Vβ usage in postimmunization PBMC was alsocompared with PBMC cultured with recombinant HBsAg(rHBsAg). The level of in vitro T-cell proliferation in the presence of rHBsAg increasedsignificantly (P < 0.01) in PBMC isolated aftervaccinations. Increases in the different TcR Vβgenes were also observed in each individual followingvaccinations, regardless of the similarity in their HLAalleles. Specific HBV-related antigen-responsive T cellswere induced after HB vaccination, without any commonrestriction for the TcR Vβ gene families. The mechanism that helps prevent HBV infection wasthus found to involve multiclonal alterations in the TcRVβ repertoire.
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REFERENCES
McIntyre B, Allison JP: The mouse T-cell receptor: Structural heterogeneity of molecules of normal T cells defined by xenoantiserum. Cell 34:739-746, 1983
Meuer SC, Fitzgerald KA, Hussey RE, Hodgdon JC, Schlossman SF, Reinherz EL: Clonotypic structures involved in antigen-specific human T-cell function. J Exp Med 157:705-719, 1983
Ben-Nun A, Liblau RS, Cohen L, Lehmann D, Tournier-Lasserve E, Rosenzweig A, Jingwu Z, Raus JCM, Bach MA: Restricted T-cell receptor V beta usage by myelin basic protein-specific T-cell clones in multiple sclerosis: Predominant genes vary in individuals. Proc Natl Acad Sci USA 88:2466-2470, 1991
Dalgleish AG, Wilson S, Gompels M, Ludlam C, Gazzard B, Coates AM, Habeshaw J: T-cell receptor variable gene products and early HIV-1 infection. Lancet 339:824-828, 1992
Lafon M, Lafage M, Martinez-Arends A, Ramirez R, Vuillier F, Charron D, Lotteau V, Scott-Algara D: Evidence for a viral superantigen in humans. Nature 358:507-510, 1992
Fink PJ, Matis LA, McElligott DL, Bookman M, Hedrick SM: Correlations between T-cell specificity and the structure of the antigen receptor. Nature 321:219-226, 1986
Nakamura K, Yuh K, Sugyo S, Shijo H, Kimura N, Okumura M: Apoptosis observed in peripheral T lymphocytes from patients with chronic hepatitis B. Gastroenterology 111:156-164, 1996
Dudley FJ, Fox RA, Sherlock S: Cellular immunity and hepatitis-associated, Australia antigen liver disease. Lancet 1:723-726, 1972
Millich DR: Immune response to hepatitis B virus proteins: Relevance of the murine model. Semin Liver Dis 11:93-112, 1991
Margolis HS: Prevention of acute and chronic liver disease through immunization: Hepatitis B and beyond. J Infect Dis 168:9-14, 1993
Kaneshige T, Hashimoto M, Matsumoto Y, Kinoshita T, Hirasawa T, Uchida K, Inoko H: Serologic and nucleotide sequencing analysis of a novel DR52 associated DRB1 allele with the DR “NJ25” specificity, designated DRB1*1307. Hum Immunol 41:151-159, 1994
Maeda M, Maruyama N, Ishii H, Uryu N, Ota M, Tsuji K, Inoko H: A simple and rapid method for HLA-DQA1 genotyping by digestion of PCR-amplified DNA with allele-specific restriction endonucleases. Tissue Antigens 34:290-298, 1989
Sengar DPS, Goldstein R: Comprehensive typing of DQB1 alleles by PCR-RFLP. Tissue Antigens 43:242-248, 1994
Fukumori Y, Nagao N, Ohnoki S, Shibata H, Okubo Y, Yamaguchi H: A polymerase chain reaction-restriction fragment length polymorphism method with allele-specific primers for HLA-DPB1 genotyping. InHLA 1991, Vol 2. K Tsuji, M Aikawa, T Sasazuki (eds). Oxford, Oxford Science Publications, 1992, pp 331-333
Bodmer JG, Marsh SGE, Albert ED, Bodmer WF, Bontrop RE, Charron D, Dupont B, Erlich HA, Mach B, Mayr WR, Parham P, Sasazuki T, Schreuder GMT, Strominger JL, Sve jgaard A, Terasaki PI: Nomenclature for factors of the HLA systems. Tissue Antigens 46:1-18, 1995
Wysocki LJ, Sato VL: “Panning” for lymphocytes: A method for cell selection. Proc Natl Acad Sci USA 75:2844-2848, 1978
Miyanohara A, Toh-e A, Nozaki C, Hamada F, Ohtomo N, Matsubara K: Expression of hepatitis B surface antigen in yeast. Proc Natl Acad Sci USA 80:1-5, 1983
Chomczynski P, Sacchi N: Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156-159, 1987
Kimura N, Toyonaga B, Yoshikai Y, Du RP, Mak TW: Sequences and repertoire of the human T-cell receptor a and b chain variable region genes in thymocytes. Eur J Immunol 17:375-383, 1987
Kimura N, Toyonaga B, Yoshikai Y, Triebel F, Debre P, Minden MD, Mak TW: Sequences and diversity of human T cell receptor ? chain variable region genes. J Exp Med 164:739-750, 1986
Choi Y, Kotzin B, Herron L, Callahan J, Marrack P, Kappler J: Interaction of Staphylococcus aureustoxin “superantigens” with human T cells. Proc Natl Acad Sci USA 86:8941-8945, 1989
Sambrook J, Fritsch EF, Maniatis T: Analysis and cloning of eukaryotic genomic DNA. InMolecular cloning, Vol 2, 2nd ed. C Nolan (ed). New York, Cold Spring Harbor Press, 1989, pp 9-58
Dong RP, Kimura A, Numano F, Nishimura Y, Sasazuki T: HLA-linked susceptibility and resistance to Takayasu arteritis. Heart Vessels 7(suppl):73-80, 1992
Dong RP, Kimura A, Hashimoto H, Akizuki M, Nishimura Y, Sasazuki T: Difference in HLA-linked genetic background between mixed connective tissue disease and systemic lupus erythematosus. Tissue Antigens 41:20-25, 1993
Woolf B: On estimating the relation between blood group and disease. Ann Hum Genet 19:251-253, 1955
Murillo LA, Tenjo FA, Clavijo OP, Orozco MA, Sampaio S, Kalil J, Patarroyo ME: A specific T-cell receptor genotype preference in the immune response to a synthetic Plasm odium falciparummalaria vaccine. Parasite Immunol 14:87-94, 1992
Hibberd ML, Wong FS, Nicholson LB, Demaine AG: Analysis of human T-cell receptor Vb gene usage following immunization to tetanus toxoid in vivo.Immunology 79:398-402, 1993
Goudeau A, Denis F, Mounier M, Dubois F, Klein J, Godefroy A, Ballet M, Mountij A: Comparative multicentre study of the immunogenicity of different hepatitis B vaccines in healthy volunteers. Postgrad Med J 63(suppl 2):125-128, 1987
Ferrari C, Bertoletti A, Penna A, Cavalli A, Valli A, Missale G, Pilli M, Fowler P, Giuberti T, Chisari FV, Fiaccadori F: Identification of immunodominant T-cell epitopes of the hepatitis B virus nucleocapsid antigen. J Clin Invest 88:214-222, 1991
Germain RN: The second class story. Nature 353:605-606, 1991
Barbey C, Watts C, Corradin G: Antigen-processing organelles from DRB1*1101 and DRB1*1104 B cell lines display a differential degradation activity*. Eur J Immunol 25:30-36, 1995
Clothia C, Boswell DR, Lesk AM: The outline structure of the T-cell ??? receptor. EMBO J 7:3745-3755, 1988
Kayser C, Waase I, Weyand CM, Goronzy JJ: T-cell receptor germline gene segments and HLA haplotypes control the length of the CDR3 of human T-cell receptor b chains. Cell Immunol 168:235-242, 1996
Russell JH, White CL, Loh DY: Receptor-stimulated death pathway is opened by antigen in mature T cells. Proc Natl Acad Sci USA 88:2151-2155, 1991
Ucker DS, Meyers J, Obermiller PS: Activation-driven T-cell death. 2. Quantitative differences alone distinguish stimuli triggering nontransformed T-cell proliferation or death. J Immunol 149:1583-1592, 1992
Takahashi T, Tanaka M, Brannan CI, Jenkins NA, Copeland NG, Suda T, Nagata S: Generalized lympho-proliferative disease in mice, caused by a point mutation in the Fas ligand. Cell 76:969-976, 1994
Owen-Schaub LB, Yonehara S, Crump WL III, Grimm EA: DNA fragmentation and cell death is selectively triggered in activated human lymphocytes by Fas antigen engagement. Cell Immunol 140:197-205, 1992
Suda T, Okazaki T, Naito Y, Yokota T, Arai N, Ozaki S, Nakao K, Nagata S: Expression of Fas ligand in cells of T cell lineage. J Immunol 154:3806-3813, 1995
Nagata S, Goldstein P: The Fas death factor. Science 267:1449-1456, 1995
Ferrari C, Penna A, Giuberti T, Tong MJ, Ribera E, Fiaccadori F, Chisari FV: Intrahepatic, nucleocapsid antigen-specific T cells in chronic active hepatitis B. J Immunol 139:2050-2058, 1987
Casini A, Alberti A, Banchetti E, Schiavon E, Patussi V, Pontisso P, Surrenti C, Realdi G: Cell-mediated immunity to HBcAg in chronic HBV infection. Am J Clin Pathol 88:494-498, 1987
Tsai SL, Chen PJ, Lai MY, Yang PM, Sung JL, Huang JH, Hwang LH, Chang TH, Chen DS: Acute exacerbations of chronic type B hepatitis are accompanied by increased T-cell responses to hepatitis B core and e antigens. J Clin Invest 89:87-96, 1992
Cassani F, Valentini P, Cataleta M, Manotti P, Francesconi R, Giostra F, Bianchi FB: Ultrasound-detected deep abdominal lymph nodes in chronic hepatitis C: High frequency and relationship with viremia. J Hepatol 23(suppl 1):143, 1995 (abstract)
Lechmann M, Ihlenfeldt HG, Braunschweiger I, Giers G, Jung G, Matz B, Kaiser R, Sauerbruch T, Spengler U: T-and B-cell responses to different hepatitis C virus antigens in patients with chronic hepatitis C infection and in healthy anti-hepatitis C virus-positive blood donors without viremia. Hepatology 24:790-795, 1996
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Yuh, K., Sugyo, S., Nakamura, K. et al. Analysis of Human T-Cell Antigen Receptor Variable β Gene Usage Following Vaccination with Recombinant HBsAg. Dig Dis Sci 43, 880–886 (1998). https://doi.org/10.1023/A:1018846921408
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DOI: https://doi.org/10.1023/A:1018846921408