Skip to main content
SpringerLink
Log in

Molecular Identification of a Murine Ubiquitin/60S Ribosomal Fusion Protein and Expression Study in Mouse Kidney

  • Published:
Biochemical Genetics Aims and scope Submit manuscript

    We’re sorry, something doesn't seem to be working properly.

    Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

Abstract

We have isolated and sequenced a 500-bp cDNAclone which is the murine homologue of a ubiquitin/60Sribosomal fusion protein. The cDNA and the deduced aminoacid sequence are highly conserved. Northernblotting demonstrates constitutive expressionin a mouse renal epithelial cell line and in mousekidneys. Upon stimulation with interferon-gamma orphorbol esters, the expression is not changed,contrastingwith the stimulation seen for polyubiquitin.Expression of the 16-kD ubiquitin/60S protein is alsodemonstrated in mouse epithelial cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  • Baker, R. T., and Board, P. G. (1991). The human ubiquitin-52 amino acid fusion protein gene shares several structural features with mammalian ribosomal protein genes. Nucleic Acids Res. 19:1035.

    Google Scholar 

  • Benz, P. S., Fan, X. H., and Wüthrich, R. P. (1996). Enhanced tubular epithelial CD44 expression in MRL-lpr lupus nephritis. Kidney Int. 50:156.

    Google Scholar 

  • Finch, J. S., St. John, T., Krieg, P., Bonham, K., Smith, H. T., Fried, V. A., and Bowden, G. T. (1992). Overexpression of three ubiquitin genes in mouse epidermal tumors is associated with enhanced cellular proliferation and stress. Cell Growth Different. 3:269.

    Google Scholar 

  • Jennissen, H. P. (1995). Ubiquitin and the enigma of intracellular protein degradation. Eur. J. Biochem. 231:1.

    Google Scholar 

  • Muller, S., and Schwartz, L. M. (1995). Ubiquitin in homeostasis, development and disease. Bioessays 17:677.

    Google Scholar 

  • Nenoi, M. (1992). Induced accumulation of polyubiquitin gene transcripts in HeLa cells after UV-irradiation and TPA-treatment. Int. J. Radiat. Biol. 61:205.

    Google Scholar 

  • Oertli, B., Beck-Schimmer, B., Fan, X., and Wüthrich, R. P. (1998). Mechanisms of hyaluronan-induced up-regulation of ICAM-1 and VCAM-1 expression by murine kidney tubular epithelial cells: Hyaluronan triggers cell adhesion molecule expression through a mechanism involving activation of nuclear factor-κB and activating protein-1. J. Immunol. 161:3431.

    Google Scholar 

  • Redman, K. L., and Burris, G. W. (1996). The cDNA for the ubiquitin-52-amin o-acid fusion protein from rat encodes a previously unidentified 60S ribosomal subunit protein. Biochem. J. 315:315.

    Google Scholar 

  • St. John, T., Gallatin, W. M., Siegelman, M., Smith, H. T., Fried, V. A., and Weissman, I. L. (1986). Expression cloning of a lymphocyte homing receptor cDNA: Ubiquitin is the reactive species. Science 231:845.

    Google Scholar 

Download references

Authors
  1. Lin Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Rudolf P. Wuthrich
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sun, L., Wuthrich, R.P. Molecular Identification of a Murine Ubiquitin/60S Ribosomal Fusion Protein and Expression Study in Mouse Kidney. Biochem Genet 37, 139–147 (1999). https://doi.org/10.1023/A:1018778320235

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1018778320235

Search

Navigation