Skip to main content
Log in

A New Esterase Gene Amplification Involved in OP Resistance in Culex pipiens Mosquitoes from China

  • Published:
Biochemical Genetics Aims and scope Submit manuscript

Abstract

Two overproduced esterases (A8 and B8) notpreviously described were found in southern China. Theyprovide a low resistance level to organophosphate (OP)insecticides, and correspond to a coamplification of both esterase loci (Est-2 and Est-3)classically involved in OP resistance for this mosquitospecies. This coamplification is distinct from all othersimilar events thus far reported. The peculiar situation in southern China, where numerous OP resistancealleles at these two loci were found, is discussed incomparison with the Mediterranean situation, the onlyone with a similar diversity of overproducedesterases.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

REFERENCES

  • Ben Cheikh, H., and Pasteur, N. (1993). Resistance to temephos: An organophosphate insecticide in Culex pipiens from Tunisia, North Africa. J. Am. Mosq. Control Assoc. 9:335.

    PubMed  Google Scholar 

  • Chevillon, C., Pasteur, N., Marquine, M., Heyse, D., and Raymond, M. (1995). Population structure and dynamics of selected genes in the mosquito Culex pipiens. Evolution 49:997.

    Google Scholar 

  • Curtis, C. F., and Pasteur, N. (1981). Organophosphate resistance in vector populations of Culex pipiens L. (Diptera: Culicidae). Bull. Entomol. Res. 71:153–161.

    Google Scholar 

  • Dong Yan, D. (1990). Studying on the bioassays of seven pyrethroids for the Culex pipiens. Pest Control 6:6.

    Google Scholar 

  • Finney, D. J. (1971). Probit Analysis, Cambridge University Press, England.

    Google Scholar 

  • Georghiou, G. P., Metcalf, R. L., and Gidden, F. E. (1966). Carbamate resistance in mosquitoes. Selection of Culex pipiens fatigans Wied. for resistance to Baygon. Bull. World Health Organiz. 35:691.

    Google Scholar 

  • Guillemaud, T., Rooker, S., Pasteur, N., and Raymond, M. (1996). Testing the unique amplification event and the worldwide migration hypothesis of insecticide resistance genes with sequence data. Heredity 77:535.

    PubMed  Google Scholar 

  • Guillemaud, T., Makate, N., Raymond, M., Hirst, B., and Callaghan, A. (1997). Esterase gene amplification in Culex pipiens. Insect Mol. Biol. 6:319.

    PubMed  Google Scholar 

  • Guillemaud, T., Lenormand, T., Bourguet, D., Chevillon, C., Pasteur, N., and Raymond, M. (1998). Evolution of resistance in Culex pipiens: Allele replacement and changing environment. Evolution 52:430.

    Google Scholar 

  • Marchi, A., and Addis, G. (1990). Resistenza agli insetticidi organofosfati in popolazioni sarde di Culex pipiens (Diptera: Culicidae). Parassitologia 32:177.

    PubMed  Google Scholar 

  • Mouchès, C., Pasteur, N., Bergé, J. B., Hyrien, O., Raymond, M., Robert de Saint Vincent, B., De Silvestri, M., and Georghiou, G. P. (1986). Amplification of an esterase gene is responsible for insecticide resistance in a California Culex mosquito. Science 233:778.

    PubMed  Google Scholar 

  • Mouchès, C., Pauplin, Y., Agawarl, M., Lemieux, L., Herzog, M., Abadon, M., Beyssat-Arnaouty, V., Hyrien, O., Robert de Saint Vincent, B., Georghiou, G. P., and Pasteur, N. (1990). Characterization of amplification core and esterase B1 gene responsible for insecticide resistance in Culex. Proc. Natl. Acad. Sci. USA 87:2574.

    PubMed  Google Scholar 

  • Pasteur, N., Sinègre, G., and Gabinaud, A. (1981). Est-2 and Est-3 polymorphism in Culex pipiens L. from southern France in relation to organophosphate resistance. Biochem. Genet. 19:499.

    PubMed  Google Scholar 

  • Pasteur, N., Pasteur, G., Bonhomme, F., and Britton-Davidian, J. (1988). Pratical Isozyme Genetics, Ellis Horwood, Chichester, England.

    Google Scholar 

  • Poirié, M., Raymond, M., and Pasteur, M. (1992). Identification of two distinct amplifications of the esterase B locus in Culex pipiens (L.) mosquitoes from Mediterranean countries. Biochem. Genet. 30:13.

    PubMed  Google Scholar 

  • Prabhaker, N., Coudriet, D. L., and Toscano, N. C. (1988). Effects of synergists on organophosphate and permethrin resistance in sweetpotato whitefly (Homoptera: Aleyrodidae). J. Econ. Entomol. 81:34.

    Google Scholar 

  • Qiao, C.-L., and Raymond, M. (1995).The same esterase B1 haplotype is amplified in insecticide resistant mosquitoes of the Culex pipiens complex from the Americas and China. Heredity 74:339.

    PubMed  Google Scholar 

  • Ratsira, D., Prato, G., and Raymond, M. (1993). PROBIT, CNRS-UMII, Licence L93019, Avenix, 34680 St. Georges d'Orques, France.

  • Raymond, M., and Marquine, M. (1994). Evolution of insecticide resistance in Culex pipiens populations: The Corsican paradox. J. Evol. Biol. 7:315.

    Google Scholar 

  • Raymond, M., Pasteur, N., Georghiou, G. P., Mellon, R. B., Wirth, M. C., and Hawley, M. K. (1987). Detoxification esterases new to California, USA, in organophosphate-resistant Culex quinquefasciatus (Diptera: Culicidae). J. Med. Entomol. 24:24.

    PubMed  Google Scholar 

  • Raymond, M., Beyssat-Arnaouty, V., Sivasubramanian, N., Mouchès, C., Georghiou, G. P., and Pasteur, N. (1989). Amplification of various esterase B's responsible for organophosphate resistance in Culex mosquitoes. Biochem. Genet. 27:417.

    PubMed  Google Scholar 

  • Raymond, M., Callaghan, A., Fort, P., and Pasteur, N. (1991). Worldwide migration of amplified insecticide resistance genes in mosquitoes. Nature 350:151.

    PubMed  Google Scholar 

  • Raymond, M., Qiao, C. L., and Callaghan, A. (1996). Esterase polymorphism in insecticide susceptible populations of the mosquito Culex pipiens. Genet. Res. 67:19.

    PubMed  Google Scholar 

  • Rivet, Y., Marquine, M., and Raymond, M. (1993). French mosquito populations invaded by A2-B2 esterases causing insecticide resistance. Biol. J. Linn. Soc. 49:249.

    Google Scholar 

  • Rooker, S., Guillemaud, T., Bergé, J., Pasteur, N., and Raymond, M. (1996). Coamplification of esterase A and B genes as a single unit in the mosquito Culex pipiens. Heredity 77:555.

    PubMed  Google Scholar 

  • Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.

    Google Scholar 

  • Severini, C., Romi, R., Marinucci, M., and Raymond, M. (1993). Mechanisms of insecticide resistance in field populations of Culex pipiens from Italy. J. Am. Mosq. Control Assoc. 9:164.

    PubMed  Google Scholar 

  • Severini, C., Romi, R., Marinucci, M., Guillemaud, T., and Raymond, M. (1997). First record of A5-B5 esterases in an organophosphate-resistant field population of Culex pipiens from Italy. Med. Vet. Entomol. 11:123.

    PubMed  Google Scholar 

  • Vaughan, A., and Hemingway, J. (1995). Mosquito carboxylesteras e Esta2 (A2). Cloning and sequence of the full-length cDNA for a major insecticide resistance gene worldwide in the mosquito Culex quinquefasciatus. J. Biol. Chem. 270:17044.

    PubMed  Google Scholar 

  • Villani, F., Majori, G., and Romi, R. (1982). Osservazioni preliminari sui livelli di sensibilitá agli esteri fosforici in popolazioni italiane del complesso Culex pipiens. Parassitologia 24:245.

    PubMed  Google Scholar 

  • Villani, F., Urbanelli, S., Gad, A., Nudelman, S., and Bullini, L. (1986). Electrophoretic variations of Culex pipiens from Egypt and Israel. Biol. J. Linn. Soc. 29:49.

    Google Scholar 

  • Wirth, M., Marquine, M., Georghiou, G. P., and Pasteur, N. (1990). Esterase A2 and B2 in Culex quinquefasciatus (Diptera: Culicidae): Role in organophosphate resistance and linkage. J. Econ. Entomol. 27:202.

    Google Scholar 

  • Xu, J., Qu, F., and Liu, W. (1994). Diversity of amplified esterase B genes responsible for organophosphate resistance in Culex quinquefasciatus from China. J. Med. Coll. PLA 9:20.

    Google Scholar 

Download references

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Qiao, CL., Marquine, M., Pasteur, N. et al. A New Esterase Gene Amplification Involved in OP Resistance in Culex pipiens Mosquitoes from China. Biochem Genet 36, 417–426 (1998). https://doi.org/10.1023/A:1018757815692

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1018757815692

Navigation