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Evolution of the Amylase Isozymes in the Drosophila melanogaster Species Subgroup

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Abstract

The relationship between the net charge ofmolecules and their mobility on electrophoresis wasanalyzed for Drosophila alpha-amylases. Most ofthe differences in electrophoretic mobility, 98.2%, canbe explained by the charge state. Therefore fivereference amino acid sites, which are informativeresidues for charge differences among amylase isozymes,were considered for the evolution of the isozymes in Drosophila melanogaster. Theamylase isozymes in D. melanogaster can beclassified into three groups, I (AMY1,AMY2, and AMY3-A), II(AMY3-B and AMY4), and III(AMY5, AMY6-A, andAMY6-B), based on the differences in the reference sites. The mostprimitive amylase in D. melanogaster was foundto belong to Group I, most likely the AMY2isozyme. Groups II and III could have been derived fromGroup I. These results were confirmed by the analysis of 38amino acid sites with charge differences inDrosophila.

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Authors
  1. Yoshinori Matsuo
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  2. Nobuyuki Inomata
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  3. Tsuneyuki Yamazaki
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Matsuo, Y., Inomata, N. & Yamazaki, T. Evolution of the Amylase Isozymes in the Drosophila melanogaster Species Subgroup. Biochem Genet 37, 289–300 (1999). https://doi.org/10.1023/A:1018755109757

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  • DOI: https://doi.org/10.1023/A:1018755109757

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