Abstract
The regulation of amylase activity in threeDrosophila species, D. melanogaster,D. funebris and D. saltans, wasanalyzed by measuring the specific activity levels infour dietary environments, cornmeal, glucose, 5% starch, and 10% starch, at threedevelopmental stages, i.e., the third-instar larval,pupal, and 2-day-old adult stages. The developmentalprofiles of amylase activity for the threeDrosophila species showed that the level of activity washigh at the larval and adult stages but substantiallylow at the pupal stage, suggesting thatDrosophila does not utilize starch at the pupalstage. Divergence in the regulation of amylase was observed amongthe three Drosophila species on the followingpoints. (1) The order of amylase specific activity wasD. melanogaster > D. funebris >D. saltans. (2) The response pattern to the dietary environment varied amongthe species and changed during development. (3) Thetiming of the switch in the response pattern to thedietary environment during development was before pupation in D. funebris and D.saltans but after pupation in D.melanogaster. The significance of the divergence inthe regulation of amylase activity for adaptation to astarch environment in Drosophila is discussed.
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LITERATURE CITED
Abe, K. (1958). Genetical and biochemical studies on amylase in Drosophila melanogaster. Jap. J. Genet. 33:138.
Da Lage, J. L., Lemeunier, F., Cariou, M. L., and David, J. R. (1992). Multiple amylase genes in Drosophila ananassae and related species. Genet. Res. 59:85.
Doane, W.W. (1967). Quantitation of amylases in Drosophila separated by acrylamide gel electrophoresis. J. Exp. Zool. 164:363.
Doane, W. W. (1969a). Amylase variants in Drosophila melanogaster. Linkage studies and characterization of enzyme extracts. J. Exp. Zool. 171:321.
Doane, W. W. (1969b). Drosophila amylase and problems in cellular differentiation. In Hanley, E. W. (ed.), RNA in Development, University of Utah Press, Salt Lake City.
Fujimoto, J., Kanou, C., Eguchi, Y., and Matsuo, Y. (1999). Adaptation to a starch environment and regulation of α-amylase in Drosophila. Biochem. Genet. 37:53.
Hickey, D. A., and Benkel, B. F. (1982). Regulation of amylase activity in Drosophila melanogaster. Effects of dietary carbohydrate. Biochem. Genet. 20:1117.
Inomata, N., Kanda, K., Cariou, M. L., Tachida, H., and Yamazaki, T. (1995). Evolution of the response patterns to dietary carbohydrates and the developmental differentiation of gene expression of α-amylase in Drosophila. J. Mol. Evol. 41:1076.
Inomata, N., Tachida, H., and Yamazaki, T. (1997). Molecular evolution of the Amy multigenes in the subgenus Sophophora of Drosophila. Mol. Biol. Evol. 14:942.
Kikkawa, H., and Abe, K. (1960). Genetic control of amylase in Drosophila melanogaster. Annot. Zool. Jpn. 33:14.
Matsuo, Y., and Yamazaki, T. (1984). Genetic analysis of natural populations of Drosophila melanogaster in Japan. IV. Natural selection on the inducibility, but not on the structural genes, of amylase loci. Genetics 108:879.
Matsuo, Y., and Yamazaki, T. (1986). Genetic analysis of natural populations of Drosophila melanogaster in Japan. VI. Differential regulation of duplicated amylase loci and degree of dominance of amylase activity in different environments. Jap. J. Genet. 61:543.
Matsuo, Y., and Yamazaki, T. (1997). Genetic factors on the second and third chromosomes responsible for the variation of amylase activity and inducibility in Drosophila melanogaster. Genet. Res. 70:97.
Milanovic, M., and Andjelkovic, M. (1992). Adaptive significance of amylase polymorphism in Drosophila. VI. Properties of two amylase variants and the effect of food components on amylase activity in Drosophila subobscura. Comp. Biochem. Phys. 101B:611.
Milanovic, M., and Andjelkovic, M. (1993). Biochemical and genetic diversity of alpha-amylase in Drosophila. Arch. Biol. Sci. Belgrade 45:63.
Milanovic, M., Stamenkovic-Bojic, G., and Andjelkovic, M. (1989). Adaptive significance of amylase polymorphism in Drosophila. IV. A comparative study of biochemical properties of the alpha-amylase in Drosophila melanogaster, Drosophila hydei, Drosophila subobscura and Drosophila busckii. Comp. Biochem. Phys. 93B:629.
Prigent, S., Matoub, M., Rouland, C., and Cariou, M. L. (1998). Metabolic evolution in alpha-amylases from Drosophila virilis and D. repleta, two species with different ecological niches. Comp. Biochem. B. Biochem. Mol. Biol. 119:407.
Shibata, H., and Yamazaki, T. (1994). A comparative study of the enzymological features of α-amylase in the Drosophila melanogaster species subgroup. Jap. J. Genet. 69:251.
Yamazaki, T. (1986). Genetic analysis of natural populations of Drosophila melanogaster in Japan. V. Genetic variabilities of amylase activities in different developmental stages and their relation to fitness. Jap. J. Genet. 61:329.
Yamazaki, T., and Matsuo, Y. (1984). Genetic analysis of natural populations of Drosophila melanogaster in Japan. III. Genetic variability of inducing factors of amylase and fitness. Genetics 108:223.
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Eguchi, Y., Matsuo, Y. Divergence of the Regulation of alpha-Amylase Activity in Drosophila melanogaster, Drosophila funebris, and Drosophila saltans. Biochem Genet 37, 41–52 (1999). https://doi.org/10.1023/A:1018714000671
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DOI: https://doi.org/10.1023/A:1018714000671