Abstract
A new isotope-assisted cross-relaxation editing experiment, [1H-13C]DINE-NOESY[1H-15N]HSQC (DINE = Double INEPT Edited), is proposed. It is based on the selectiveinversion of CH/CH3 or CH2 protons in the middle of the mixing time. The experiment sortsout the spin diffusion paths according to the principal mediators, either the CH/CH3 or theCH2 protons. This is useful in the structure refinement process, as it enables proper alignmentof the aliphatic protons in the vicinity of NH protons.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bax, A., Ikura, M., Kay, L.E., Torchia, D.A. and Tschudin, R. (1990) J. Magn. Reson., 86, 304–318.
Bodenhausen, G. and Ruben, D.J. (1980) Chem. Phys. Lett., 69, 185–190.
Clore, G.M. and Gronenborn, A.M. (1991) Science, 252, 1390–1399.
Fejzo, J., Krezel, A.M., Westler, W.M., Macura, S. and Markley, J.L. (1991) J. Magn. Reson., 92, 651–657.
Fejzo, J., Westler, W.M., Markley, J.L. and Macura, S. (1992) J. Am. Chem. Soc., 114, 1523–1524.
Griffey, R.H. and Redfield, A.G. (1987) Q. Rev. Biophys., 19, 51–82.
Ikura, M., Bax, A., Clore, G.M. and Gronenborn, A.M. (1990) J. Am. Chem. Soc., 112, 9020–9022.
Ikura, M. and Bax, A. (1992) J. Am. Chem. Soc., 114, 2433–2440.
Kalk, A. and Berendsen, H.J.C. (1976) J. Magn. Reson., 24, 343–366.
Macura, S. and Ernst, R.R. (1980) Mol. Phys., 41, 95–117.
Macura, S., Fejzo, J., Hoogstraten, C.G., Westler, W.M. and Markley, J.L. (1992) Isr. J. Chem., 32, 245–256.
Marion, D., Driscoll, P.C., Kay, L.E., Wingfield, P.T., Bax, A., Gronenborn, A.M. and Clore, G.M. (1989) Biochemistry, 28, 6150–6156.
Massefski Jr., W. and Redfield, A.G. (1988) J. Magn. Reson., 78, 150–155.
Norwood, T., Boyd, J., Heritage, J.E., Soffe, N. and Campbell, I. (1990) J. Magn. Reson., 87, 488–501.
Otting, G. and Wüthrich, K. (1990) Q. Rev. Biophys., 23, 39–96.
Vijay-Kumar, S., Bugg, C. and Cook, W. (1987) J. Mol. Biol., 194, 531–544.
Vincent, S., Zwahlen, C., Bolton, P.H., Logan, T. and Bodenhausen, G. (1996) J. Am. Chem. Soc., 118, 3531–3532.
Zolnai, Z., Juranic, N., Markley, J.L. and Macura, S. (1995) Chem. Phys., 200, 161–179.
Zwahlen, C., Vincent, S., Di Bari, L., Levitt, M. and Bodenhausen, G. (1994) J. Am. Chem. Soc., 116, 362–368.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Juranic, N., Zolnai, Z. & Macura, S. Identification of spin diffusion pathways in proteins by isotope-assisted NMR cross- relaxation network editing. J Biomol NMR 9, 317–322 (1997). https://doi.org/10.1023/A:1018639211400
Issue Date:
DOI: https://doi.org/10.1023/A:1018639211400