Abstract
A simple and efficient technique for in vitro unpollinated ovary culture in rice which is also applicable for indica genotypes was developed for breeding and genetic studies. Sampling explants at the auricle distance of 7–12 cm between the two uppermost leaves of a tiller, providing a chilling pretreatment and ovaries with 1/3 of the hulls intact gave optimum response to culture. For callus induction with the spontaneous breaking of ovaries, N6 media supplemented with NAA (2 mg/l) and DMSO (0.6–0.8%) gave a mean PCI value of 3.8% and range of 0.8–12.5% among genotypes. Media combining 2,4,5-T or 2,4-D with NAA in N6 medium also has reasonably good callus induction. For calli induced inside, 2,4-D (0.2–0.5 mg/l), NAA (2 mg/l) and KT (1 mg/l) contained media were superior. The maximum green plant regeneration (PPR) of 77.3% was found with the medium containing NAA 0.25 mg/l, IAA 0.5 mg/l and KT 2.0 mg/l. Significant genotype, medium and their interaction effects for per cent ovary survival and callus induction were observed.
Similar content being viewed by others
References
Ao, G.M., S.X. Zhao & G.H. Li, 1982. In vitroinduction of haploid plantlets from unpollinated ovaries of corn (Zea maysL.). Acta Genet Sin 9 (4): 281-283.
Asselin, M.M. de, 1980. Obtention d'haploides in vitroà partir d’ovaires non fecondes de riz, Oryza sativaL. CR Acad Sci Paris Ser D 296: 498-492.
Castillo, A.M. & L. Cistue, 1993. Production of gynogenic haploids of Hordeum vulgareL. Plant Cell Rep 12 (3): 139-143.
Chen, C.C. & C.M. Lin, 1981. Genotypic differences in plant production in anther culture of rice. In: W.C. Chang (Ed.), Proc Symp Plant Cell Tissue Culture, pp. 199-203. Acad Sin, Taipei.
Chu, C., 1978. The N6 medium and its application to anther culture of cereal crops. In: Proceedings of Symposium on Plant Tissue Culture, pp. 43-50. Science Press, Peking.
Genovesi, A.D. & C.W. Majill, 1979. Improved rate of callus and green plant production from rice anther culture following cold shock. Crop Sci 19: 662-664.
Gomez, K.A. & A.A. Gomez, 1984. Statistical Procedures for& Agriculture Research (2nd edition), pp. 199-205. John Wiley Sons.
Hosemanns, D. & D. Bossoutrot, 1983. Induction of haploid plants from in vitroculture of unpollinated beet ovules (Beta vulgarisL.). Plant Breeding 91: 74-77.
Li, C.C., H. Cheng & R.L. Song, 1983. A preliminary study of in vitroparthenogenesis ovary culture in rice. Fujian Nongye Keji, Fujian Agric Sci Technol 4: 25-27.
San Noeum, L.H., 1976. Haploides d'Hordeum vulgareL. par culture in vitrod'ovaires non fecondes. Ann Amélior Plantes 26: 751-754.
Svirshchevskaya, A.M. & V.E. Bormotov, 1994. Features of regeneration following gynogenesis in sugarbeet. Doklady Akademii Nauk Belarusi 38 (6): 57-59.
Tao, Z.R., M.S. Liu & Z.C. Zhu, 1985. In vitroproduction of haploid plantlets from unpollinated ovaries of potato. Hereditas 7 (5): 24.
Tian, H.Q. & H.Y. Yang, 1983. Synergid apogamy and egg cell anomalous division in cultured ovaries of Oryza sativaL. Acta Bot Sin 25: 403-408.
Yan, H., J. Dong, C. Zhou & H.Y. Yang, 1987. Regulation of in vitroparthenogenesis and somatic proliferation in sunflower by several factors. Acta Bot Sin 29 (6): 580-587.
Zhou, C. & H.Y. Yang, 1980. In vitroinduction of haploid plantlets from unpollinated young ovaries of Oryza sativaL. Acta Genet Sin 7 (3): 287-288.
Zhou, C. & H.Y. Yang, 1981. In vitroembryogenesis in unfertilized embryo sacs of Oryza sativaL. Acta Bot Sin 23 (3): 176-179.
Zhu, Z.C. & H.S. Wu, 1979. In vitroproduction of haploid plantlets from the unpollinated ovaries of Triticum aestivumand Nicotiana tabacum. Acta Genet Sin 6 (2): 181-183
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rongbai, L., Pandey, M., Garg, G. et al. Development of a technique for in vitro unpollinated ovary culture in rice, Oryza sativa L.. Euphytica 104, 159–166 (1998). https://doi.org/10.1023/A:1018613121306
Issue Date:
DOI: https://doi.org/10.1023/A:1018613121306