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Characterization of xanthans from selected Xanthomonas strains cultivated under constant dissolved oxygen

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Abstract

Seventeen wild-type Xanthomonas isolates were screened in terms of broth viscosity in shake-flasks. As culture conditions affect polymer characteristics, a fair comparison among isolates required their cultivation in a fermenter under controlled dissolved oxygen tension. Three isolates and a reference strain were studied. The mean molecular weights and molecular weight distributions of their xanthans were determined. Products showed different pyruvate (0.2–7%), acetate (5–10%) and proteinaceous nitrogen (1–3%) contents. The selected isolates exhibit properties which could improve xanthan gum production and some could be used to produce polymers with specific characteristics.

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References

  • Callet, F., Milas, M. & Rinaudo, M. 1987 Influence of acetyl and pyruvate contents on rheological properties of xanthan gum in dilute solution. International Journal of Biological Macromolecules 9, 291-293.

    Google Scholar 

  • Flores, F., Torres, L.G. & Galindo, E. 1994 Effect of the dissolved oxygen tension during cultivation of X. Campestris on the production and quality of xanthan gum. Journal of Biotechnology 34, 165-173.

    Google Scholar 

  • Galindo, E. 1994a Aspects of the process for xanthan production, Transactions of the Institute of Chemical Engineering (part C) 72, 227-237.

    Google Scholar 

  • Galindo, E. 1994b Engineering and microbiological aspects of the production of microbial polysaccharides: xanthan as a model. In Bioproducts Processing: Technology for the Tropics, ed Hashim, A.M., Institute of Chemical Engineering Symposium Series, Vol. 137, pp. 169-177.

  • Galindo, E., Salcedo, G., Flores, C. & Ramirez, M.E. 1993 Improved shake-flask test for the screening of xanthan-producing microorganisms. World Journal of Microbiology and Biotechnology 9, 122-124

    Google Scholar 

  • Galindo, E., Salcedo, G. & Ramirez, M.E. 1994 Preservation of Xanthomonas campestris on agar slopes: effects on xanthan production. Applied Microbiology and Biotechnology, 40: 634-637.

    Google Scholar 

  • Galindo, E., Torrestiana, B. & Garcia-Rejón 1989 Rheological characterization of xanthan fermentation broths and their reconstituted solutions. Bioprocess Engineering 4, 113-118.

    Google Scholar 

  • Graham, H.D. 1971 Microdetermination of Keltrol (xanthan gum). Journal of Dairy Science 54, 1162-1128.

    Google Scholar 

  • Hadjivassiliou, A.A. & Rieder, S.V. 1968 The enzymatic assay of pyruvic and lactic acid. A definitive procedure. Clinica Chimica Acta 19, 357-361.

    Google Scholar 

  • Herbst, H., Peters, H.U., Suh, I.S., Schumpe, A. & Deckwer, W.D. 1988 Monitoring xanthan quality during fermentation by size exclusion chromatography. Biotechnology Techniques 2 (2), 101-104.

    Google Scholar 

  • Herbst, H., Schumpe, A. & Deckwer, W.D. 1992 Xanthan production in stirred tank fermenters: oxygen transfer and scale-up. Chemical Engineering and Technology 15, 425-434.

    Google Scholar 

  • Hwang, J. & Kokini, J.L. 1991 Structure and rheological function of the side branches of carbohydrate polymers. Journal of Texture Studies 22, 123-167.

    Google Scholar 

  • Kennedy, J.F. & Bradshaw, I.J. 1984 Production, properties and applications of xanthan. Progress in Industrial Microbiology 19, 319-371.

    Google Scholar 

  • Kennedy, A.F.D. & Sutherland, I.W. 1987 Analysis of bacterial exopolysaccharides. Biotechnology and Applied Biochemistry 9, 12-19.

    PubMed  Google Scholar 

  • Kleinitz, W., Littmann, W. & Herbst, H. 1989 Screening of xanthan-biopolymer for a high salinity oil reservoir. Proceedings of the Fifth European Symposium on Improved Oil Recovery, 25-27 April 1989 pp. 1-9. Budapest, Hungary.

  • McComb, E.A. & McCready, R.M. 1957 Determination of acetyl in pectin and in acetylated carbohydrate polymers. Hidroxamic acid reaction. Analytical Chemistry 29, 819-821.

    Google Scholar 

  • Montes, A.L. 1966 Bromatología. Vol. I, pp. 58-60. Argentina: Ed. Universitaria de Buenos Aires.

  • Moraine, R.A. & Rogovin, P. 1971 Xanthan biopolymer production at increased concentration by pH control. Biotechnology and Bioengineering 13, 381-391.

    Google Scholar 

  • Morris, E.R. 1977 Molecular origin of xanthan solution properties. In Extracellular Microbial Polysaccharides, eds Sandford, P.A. & Laskin, A., ACS Symp. Series, Vol. 45, pp. 81-89. Washington, DC: American Chemical Society.

    Google Scholar 

  • Nitschke, M. & Thomas, R.W.S.P. 1995 Xanthan gum production by wild-type isolates of Xanthomonas campestris. World Journal of Microbiology and Biotechnology 11, 502-504.

    Google Scholar 

  • Ramírez, M.E., Fucikovsky, L., García-Jiménez, F., Quintero, R. & Galindo, E. 1988 Xanthan gum production by altered pathogenicity variants of Xanthomonas campestris. Applied Microbiology and Biotechnology 29, 5-10.

    Google Scholar 

  • Roseiro, J.C., Esgalhado, M.E., Amaral-CollaÇo, M.T. & Emery, A.N. 1992 Medium development for xanthan productions. Process Biochemistry 27, 167-175.

    Google Scholar 

  • SaÂnchez, A., MartõÂnez, A., Torres, L. & Galindo, E. 1992 Power consumption of three impeller combinations in mixing xanthan fermentation broths. Process Biochemistry 27, 351-365.

    Google Scholar 

  • Sandford, P.A., Pittsley, J.E., Knutson, C.A., Watson, P.R., Cadmus, M.C. & Jeanes, A. 1977 Variation in Xanthomonas campestris NRRL B-1459: characterization of xanthan products of differing pyruvic acid content. In Extracellular Microbial Polysaccharides, eds Sanford, P.A. & Laskin, A., ACS Symp. Series vol. 45, pp. 192-209. Washington, DC: American Chemical Society.

    Google Scholar 

  • Scamparini, A.R.P. & Rosato, Y.B. 1987 Production of xanthan gum by different isolates of Xanthomonas campestris. Proceedings of the 4th European Congress on Biotechnology, 1, eds Niejssel, D.M., Van der Meer, R.R. & Luyben, K.Ch.A.M. pp. 301-304. Amsterdam: Elsevier Science.

    Google Scholar 

  • Shatwell, K.P. & Sutherland, I.W. 1990 The influence of acetyl and pyruvate substituents on the helix-coil transition behaviour of xanthan. Carbohydrate Research 206, 87-103.

    Google Scholar 

  • Suh, I.S., Herbst, H., Schumpe, A. & Deckwer, W.D. 1990 The molecular weight of xanthan polysaccharide produced under oxygen limitation. Biotechnology Letters 12 (3), 201-206.

    Google Scholar 

  • Tako, M. & Nakamura, S. 1984 Rheological properties of deacetylated xanthan in aqueous media. Agriculture and Biological Chemistry 48 (12), 2987-2993.

    Google Scholar 

  • Torrestiana, B., Fucikovsky, L. & Galindo, E. 1990 Xanthan production by some Xanthomonas isolates. Letters in Applied Microbiology 10, 81-83.

    Google Scholar 

  • Xueming, Z., Nienow, A.W., Kent, C.A., Chatwin, S. & Galindo, E. 1991 Improving xanthan fermentation performance by changing agitators, Proceedings of 7th European Conference on Mixing, 18-20 September 1991, Brugge, Belgium, Vol. I, KVI vzw, pp. 277-283.

    Google Scholar 

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Authors and Affiliations

  1. Depto. de Bioingenieri´a, Instituto de Biotecnologi´a, Universidad Nacional Auto´noma de Me´xico, Apdo. Post. 510-3, Cuernavaca, Morelos, 62250, Me´xico

    A. Sa´nchez & E. Galindo

  2. Instituto de Ecologi´a, Apdo. Postal 63, Xalapa, Veracruz, 91000, Me´xico

    M.E. Rami´rez

  3. Coordinacio´n de Ingenieri´a Ambiental, Instituto de Ingenieri´a, UNAM, Ciudad Universitaria, Apdo. Postal 70-472, Coyoaca´n Me´xico, 04510, D.F. Me´xico

    L.G. Torres

Authors
  1. A. Sa´nchez
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  2. M.E. Rami´rez
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  3. L.G. Torres
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  4. E. Galindo
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Sa´nchez, A., Rami´rez, M., Torres, L. et al. Characterization of xanthans from selected Xanthomonas strains cultivated under constant dissolved oxygen. World Journal of Microbiology and Biotechnology 13, 443–451 (1997). https://doi.org/10.1023/A:1018532418417

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  • DOI: https://doi.org/10.1023/A:1018532418417

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