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Nonenzymatic glycation of cartilage proteoglycans: an in vivo and in vitro study

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Abstract

In this study we have investigated whether proteoglycans (aggrecan) are modified by nonenzymatic glycation as in collagen. Purified human aggrecan from osteoarthritic and normal human knee articular cartilage was assayed for pentosidine, a cross-link formed by nonenzymatic glycation, using reverse-phase HPLC. In addition, an in vitro study was done by incubation of purified bovine nasal cartilage aggrecan with ribose. Pentosidine was found in all the purified human aggrecan samples. 2-3% of the total articular cartilage pentosidine was found in aggrecan. Purified link protein also contained penosidine. The in vitro study led to pentosidine formation, but did not appear to increase the molecular size of the aggrecan suggesting that pentosidine was creating intramolecular cross-links. Similar amounts of glycation were found in osteoarthritic and normal cartilage. Like collagen, aggrecan and link proteins are crosslinked by nonenzymatic glycation in normal and osteoarthritic cartilage. Crosslinking could be reproduced, in vitro, by incubating aggrecan with ribose.

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References

  1. Hamlin CR, Kohn RR (1971) Biochim Biophys Acta 236: 458–67.

    PubMed  CAS  Google Scholar 

  2. Eyre DR, Paz MA, Gallop PM (1984) [Review] Annu Rev Biochem 53: 717–48.

    Article  PubMed  CAS  Google Scholar 

  3. Last JA, Armstrong LG, Reiser K (1990) Int J Biochem 22: 559–64.

    Article  PubMed  CAS  Google Scholar 

  4. Reiser KM (1991) Proc Soc Exp Biol Med 196: 17–29.

    PubMed  CAS  Google Scholar 

  5. Miyata S, Monnier VM (1992) J Clin Invest 89: 1102–12.

    PubMed  CAS  Google Scholar 

  6. Sell DR, Monnier VM (1990) J Clin Invest 85: 380–84.

    PubMed  CAS  Google Scholar 

  7. Odetti P, Fogarty J, Sell DR, Monnier VM (1992) Diabetes 41: 153–59.

    PubMed  CAS  Google Scholar 

  8. Nagraj RH, Sell DR, Prabhakaran M, Ortwerth BJ, Monnier VM (1991) Proc Natl Acad Sci USA 88: 10257–61.

    Article  Google Scholar 

  9. Hormel SE, Eyre DR (1991) Biochim Biophy Acta 1078: 243–50.

    CAS  Google Scholar 

  10. Takahashi M, Kushida K, Ohishi T, Kawana K, Hoshino H, Uchiyama A, Inoue T (1994) Arth and Rheum 37: 724–28.

    CAS  Google Scholar 

  11. Uchiyama A, Ohishi T, Takahashi M, Kushida K, Inoue T, Fujie M, Horiuchi K (1991) J Biochem 110: 714–18.

    PubMed  CAS  Google Scholar 

  12. Kirk E, Kvorning SA (1949) J Gerontol 4: 273–84.

    PubMed  CAS  Google Scholar 

  13. Schnider SL, Kohn RR (1981) J Clin Invest 67: 1630–35.

    Article  PubMed  CAS  Google Scholar 

  14. Sell DR, Monnier VM (1989) J Biol Chem 264: 21597–602.

    PubMed  CAS  Google Scholar 

  15. Maroudas A, Palla G, Gilav E (1992) Connective Tissue Research 28: 161–69.

    PubMed  CAS  Google Scholar 

  16. Neame PJ, Christner JE, Baker JR (1986) J Biol Chem 261: 3519–35.

    PubMed  CAS  Google Scholar 

  17. Fu MX, Wells-Knecht KJ, Blackledge, Lyons TJ, Thorpe SR, Baynes JW (1994) Diabetes 43: 676–82.

    PubMed  CAS  Google Scholar 

  18. Kahn A, Pottenger LA, Phillips FM (1991) J Orthop Res 9: 777–86.

    Article  PubMed  CAS  Google Scholar 

  19. Hascall VC, Sajdera SW (1969) J Biol Chem 244: 2384–96.

    PubMed  CAS  Google Scholar 

  20. Bitter T, Muir HM (1962) Anal Biochem 4: 330–34.

    Article  PubMed  CAS  Google Scholar 

  21. Tang LH, Rosenberg L, Reiner A, Poole AR (1979) J Biol Chem 254: 10523–31.

    PubMed  CAS  Google Scholar 

  22. Rosenberg L, Choi HU, Tang LH, Pal S, Johnson T, Lyons DA, Laue TM (1991) J Bio Chem 266: 7016–24.

    CAS  Google Scholar 

  23. Stegeman, Stadler S (1967) Clin Chim Acta 18: 267–73.

    Article  Google Scholar 

  24. Eyre DR, Koob TJ, Ness KP (1984) Anal Biochem 137: 380–88.

    Article  PubMed  CAS  Google Scholar 

  25. Stenesh J (1975) In Dictionary of Biochemistry pp 56. New York, Wiley Interscience Publication.

    Google Scholar 

  26. Monnier VM, Sell DR (1994) In Maillard Reactions in Chemistry, Food, and Health. (Labuza TP, Reineccius GA, Monnier VM, O'Brien J, Baynes J, eds) pp 235–243. Cambridge: The Royal Society of Chemistry.

    Google Scholar 

  27. Assoumani MB, Maxime D, Nguyen NP (1994) In Maillard Reactions in Chemistry, Food, and Health, Labuza TP, Reineccius GA, Monnier VM, O'Brien J, Baynes J, eds) pp 43–50. Cambridge: The Royal Society of Chemistry.

    Google Scholar 

  28. Monnier VM, Sell DR, Nagraj RH, Miyata S (1991) Gerontology 37: 152–65.

    Article  PubMed  CAS  Google Scholar 

  29. Bailey AJ, Sims TJ, Avery NC, Halligan EP (1995) Biochem J 305: 385–90.

    PubMed  CAS  Google Scholar 

  30. Eyre D, Dickson I, Ness K (1988) Biochem J 252: 495–500.

    PubMed  CAS  Google Scholar 

  31. Behrens F, Kraft EL, Ogema TR (1989) J Orthop Res 7: 335–43.

    Article  PubMed  CAS  Google Scholar 

  32. Kohn RR, Cerami A, Monnier VM (1984) Diabetes 33: 57–59.

    PubMed  CAS  Google Scholar 

  33. Dyer DG, Blackledge JA, Thorpe SR, Baynes JW (1991) J Biol Chem 266: 11654–60.

    PubMed  CAS  Google Scholar 

  34. Khalifah RG, Todd P, Booth AA, Yang SX, Mott JD, Hudson BG (1996) Biochem 35: 4645–54.

    Article  CAS  Google Scholar 

  35. Phillips FM, Pottenger LA, Hay RV (1990) J Orthop Res 8: 189–98.

    Article  PubMed  CAS  Google Scholar 

  36. Buckwalter JA, Roughley PJ, Rosenberg LC (1994) Microscopic Research and Technique 28: 398–408.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Section of Orthopedic Surgery, University of Chicago Medical Center, MC 6032, 5841 S. Maryland Ave, Chicago, IL, 60637, USA

    Hemlata K. Pokharna

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  1. Hemlata K. Pokharna
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  2. Lawrence A. Pottenger
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Pokharna, H.K., Pottenger, L.A. Nonenzymatic glycation of cartilage proteoglycans: an in vivo and in vitro study. Glycoconj J 14, 917–923 (1997). https://doi.org/10.1023/A:1018514727213

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