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Isolation and genetic analysis of ethanol-sensitive mutants of thermotolerant Kluyveromyces marxianus

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Biotechnology Techniques

Abstract

Six monogenic mutants of K. marxianus, affected in their ability to tolerate ethanol, were assigned to 6 loci etr1 through etr6, probably the hot spots for ethyl methane sulfonate as these loci were found mutated in each of the non-monogenic mutants as well. Differential ethanol tolerance of allelic hexagenic mutants suggests that even more than 6 genes may be involved in controlling ethanol tolerance in K. marxianus.

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Gulati, M., Dhamija, S., Gera, R. et al. Isolation and genetic analysis of ethanol-sensitive mutants of thermotolerant Kluyveromyces marxianus. Biotechnology Techniques 11, 343–346 (1997). https://doi.org/10.1023/A:1018483731793

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  • DOI: https://doi.org/10.1023/A:1018483731793

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