Ribosomal genes were mapped in Glossina austeni, G. brevipalpis, G. f. fuscipes, G. m. submorsitans, G. p. palpalis, G. pallidipes and G. tachinoides on mitotic chromosomes by fluorescence in situ hybridization (FISH) using Drosophila hydei genomic clones that contain the 28S ribosomal DNA. In all species except G. brevipalpis, the ribosomal genes were located on the long arm of autosome L1. The Y chromosomes of G. pallidipes and G. p. palpalis showed additional hybridization signals. Supernumerary chromosomes were found in G. austeni, G. brevipalpis and G. pallidipes. The C-banding pattern obtained by in situ hybridization was compared with Giemsa C-banding patterns that were published previously. The karyotype of G. brevipalpis was found to differ from that of other Glossina species, with either two or three FISH signals being obtained with a ribosomal probe, depending on the individual analysed. The rDNA genes are the first physically mapped markers in tsetse flies and will be useful for mapping approaches.
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Amos A, Dover G (1981) The distribution of repetitive DNAs; between regular and supernumerary chromosomes in species of Glossina (tsetse): A two-step process in the origin of supernumeraries. Chromosoma 8: 673–690.
Blanchetot A, Gooding RH (1994) Genetic variability and segregation analysis in Glossina morsitans morsitans (Diptera: Glossinidae) using DNA fingerprinting. Genome 37: 289–295.
Davies EDG, Southern DI (1976) Giemsa C-banding within the genus Glossina (Diptera, Glossinidae). Genetica 46: 413–418.
Feldmann U (1992) Guidelines for the rearing of tsetse flies using the membrane feeding technique. In: Ochienǵ Odero JPR, ed. Techniques for Insect Rearing for the Development of Integrated Pest Vector Management Strategies, Vol.2. Proceedings International Group Training Course. Nairobi: ICIPE, pp 449–465.
Gooding RH (1982) Classification of nine species and subspecies of tsetse flies (Diptera: Glossinidae: Glossina Wiedemann) based on molecular genetics and breeding data. Can J Zool 60: 2737–2744.
Gooding RH (1984) Tsetse genetics: a review. Quaestiones Entomologicae 20: 89–128
Gooding RH (1992) Genetic variation in tsetse flies and implications for trypanosomiasis. Parasitol Today 8: 92-95.
Gooding, RH, Rolseth BM (1992) Genetics of Glossina morsitans morsitans (Diptera: Glossinnidae). XIV. Map locations of the loci for phosphoglucomutase and glucose-6-phosphate isomerase. Genome 35: 699–701.
Gooding R, Rolseth BM (1995). Genetics of Glossina palpalis palpalis: designation of linkage groups and the mapping of eight biochemical and visible marker genes. Genome 38: 833–837.
Langley PA, Maudlin I, Leedham MP (1984) Genetic and behavioural differences between Glossina pallidipes from Uganda and Zimbabwe. Entomol Exp Appl 35: 55–60.
Maudlin, I (1970) Preliminary studies onthe karyotypes of five species of Glossina. Parasitology 61: 71–74.
Moloo SK, Kutuza SB (1970) Feeding and crop emptying in Glossina brevipalpis Newstead. Acta Trop 27: 356–377.
Nash TAM, Kernaghan RJ, Boyle JA (1966) The large-scale rearing of Glossina austeni (Newst. in the laboratory. I. The use of pregnant and non-pregnant goats as hosts. Ann Trop Med Parasitol 60: 39–47.
Renkawitz-Pohl R, Glaetzar KH, Kunz W (1980) Characterization of cloned ribosomal DNA from Drosophila hydei. Nucleic Acids Res 8: 4593–4611.
Southern DI (1980) Chromosome diversity in tsetse flies. In: Blackman RL, Hewitt GM, Ashburner M, eds. Insect Cytogenetics. Symposia of the Royal Entomological Society of London, No. 10, Oxford Blackwell Scientific Publications, pp 225–243.
Southern DI, Pell PE (1981) Cytogenetical aspects of morsitans tsetse flies with particular reference to Glossina pallidipes (Diptera, Glossinidae). Cytobios 30: 135-152.
Southern DI, Craig-Cameron TA, Pell PE (1972) A critical chromosome analysis of Glossina austeni Newst. (Diptera, Glossinidae). Bull Entomol Res 62: 195–198.
Vreysen MJB, Mramba F, Khamis I (1992) Laboratory and field observations in relation to the release of sterile Glossina austeni on Unguja (Zanzibar island. In: Tsetse Control, Diagnosis and Chemotherapy Using Nuclear Techniques. Vienna: IAEA TECDOC 634, pp. 219–230.
Wetzel H, Luger D (1978) In vitro feeding in the rearing of tsetse flies (Glossina m. morsitans and G p. palpalis, Diptera: Glossinidae) Tropenmed Parasitol 29: 239–251.
Willhoeft U, Franz G (1996) Identification of the sex-determining region of the Ceratitis capitata Y chromosome by deletion mapping. Genetics 144: 739–745.
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Willhoeft, U. Fluorescence in situ hybridization of ribosomal DNA to mitotic chromosomes of tsetse flies (Diptera: Glossinidae: Glossina). Chromosome Res 5, 262–267 (1997). https://doi.org/10.1023/A:1018471620542
- ribosomal DNA
- tsetse flies