Advertisement

Chromosome Research

, Volume 5, Issue 4, pp 262–267 | Cite as

Fluorescence in situ hybridization of ribosomal DNA to mitotic chromosomes of tsetse flies (Diptera: Glossinidae: Glossina)

  • Ute Willhoeft
Article

Abstract

Ribosomal genes were mapped in Glossina austeni, G. brevipalpis, G. f. fuscipes, G. m. submorsitans, G. p. palpalis, G. pallidipes and G. tachinoides on mitotic chromosomes by fluorescence in situ hybridization (FISH) using Drosophila hydei genomic clones that contain the 28S ribosomal DNA. In all species except G. brevipalpis, the ribosomal genes were located on the long arm of autosome L1. The Y chromosomes of G. pallidipes and G. p. palpalis showed additional hybridization signals. Supernumerary chromosomes were found in G. austeni, G. brevipalpis and G. pallidipes. The C-banding pattern obtained by in situ hybridization was compared with Giemsa C-banding patterns that were published previously. The karyotype of G. brevipalpis was found to differ from that of other Glossina species, with either two or three FISH signals being obtained with a ribosomal probe, depending on the individual analysed. The rDNA genes are the first physically mapped markers in tsetse flies and will be useful for mapping approaches.

FISH Glossina ribosomal DNA tsetse flies 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Amos A, Dover G (1981) The distribution of repetitive DNAs; between regular and supernumerary chromosomes in species of Glossina (tsetse): A two-step process in the origin of supernumeraries. Chromosoma 8: 673–690.Google Scholar
  2. Blanchetot A, Gooding RH (1994) Genetic variability and segregation analysis in Glossina morsitans morsitans (Diptera: Glossinidae) using DNA fingerprinting. Genome 37: 289–295.Google Scholar
  3. Davies EDG, Southern DI (1976) Giemsa C-banding within the genus Glossina (Diptera, Glossinidae). Genetica 46: 413–418.Google Scholar
  4. Feldmann U (1992) Guidelines for the rearing of tsetse flies using the membrane feeding technique. In: Ochienǵ Odero JPR, ed. Techniques for Insect Rearing for the Development of Integrated Pest Vector Management Strategies, Vol.2. Proceedings International Group Training Course. Nairobi: ICIPE, pp 449–465.Google Scholar
  5. Gooding RH (1982) Classification of nine species and subspecies of tsetse flies (Diptera: Glossinidae: Glossina Wiedemann) based on molecular genetics and breeding data. Can J Zool 60: 2737–2744.Google Scholar
  6. Gooding RH (1984) Tsetse genetics: a review. Quaestiones Entomologicae 20: 89–128Google Scholar
  7. Gooding RH (1992) Genetic variation in tsetse flies and implications for trypanosomiasis. Parasitol Today 8: 92-95.Google Scholar
  8. Gooding, RH, Rolseth BM (1992) Genetics of Glossina morsitans morsitans (Diptera: Glossinnidae). XIV. Map locations of the loci for phosphoglucomutase and glucose-6-phosphate isomerase. Genome 35: 699–701.Google Scholar
  9. Gooding R, Rolseth BM (1995). Genetics of Glossina palpalis palpalis: designation of linkage groups and the mapping of eight biochemical and visible marker genes. Genome 38: 833–837.Google Scholar
  10. Langley PA, Maudlin I, Leedham MP (1984) Genetic and behavioural differences between Glossina pallidipes from Uganda and Zimbabwe. Entomol Exp Appl 35: 55–60.Google Scholar
  11. Maudlin, I (1970) Preliminary studies onthe karyotypes of five species of Glossina. Parasitology 61: 71–74.Google Scholar
  12. Moloo SK, Kutuza SB (1970) Feeding and crop emptying in Glossina brevipalpis Newstead. Acta Trop 27: 356–377.Google Scholar
  13. Nash TAM, Kernaghan RJ, Boyle JA (1966) The large-scale rearing of Glossina austeni (Newst. in the laboratory. I. The use of pregnant and non-pregnant goats as hosts. Ann Trop Med Parasitol 60: 39–47.Google Scholar
  14. Renkawitz-Pohl R, Glaetzar KH, Kunz W (1980) Characterization of cloned ribosomal DNA from Drosophila hydei. Nucleic Acids Res 8: 4593–4611.Google Scholar
  15. Southern DI (1980) Chromosome diversity in tsetse flies. In: Blackman RL, Hewitt GM, Ashburner M, eds. Insect Cytogenetics. Symposia of the Royal Entomological Society of London, No. 10, Oxford Blackwell Scientific Publications, pp 225–243.Google Scholar
  16. Southern DI, Pell PE (1981) Cytogenetical aspects of morsitans tsetse flies with particular reference to Glossina pallidipes (Diptera, Glossinidae). Cytobios 30: 135-152.Google Scholar
  17. Southern DI, Craig-Cameron TA, Pell PE (1972) A critical chromosome analysis of Glossina austeni Newst. (Diptera, Glossinidae). Bull Entomol Res 62: 195–198.Google Scholar
  18. Vreysen MJB, Mramba F, Khamis I (1992) Laboratory and field observations in relation to the release of sterile Glossina austeni on Unguja (Zanzibar island. In: Tsetse Control, Diagnosis and Chemotherapy Using Nuclear Techniques. Vienna: IAEA TECDOC 634, pp. 219–230.Google Scholar
  19. Wetzel H, Luger D (1978) In vitro feeding in the rearing of tsetse flies (Glossina m. morsitans and G p. palpalis, Diptera: Glossinidae) Tropenmed Parasitol 29: 239–251.Google Scholar
  20. Willhoeft U, Franz G (1996) Identification of the sex-determining region of the Ceratitis capitata Y chromosome by deletion mapping. Genetics 144: 739–745.Google Scholar

Copyright information

© Chapman and Hall 1997

Authors and Affiliations

  • Ute Willhoeft
    • 1
  1. 1.Entomology UnitFAO/IAEA (Food Agriculture Organization International Atomic Energy Agency) Agricultural and Biotechnology LaboratorySeibersdorfAustria

Personalised recommendations