Abstract
A method that allows the performance of double-colour chromosome painting (FISH) on previously G-banded human sperm metaphases has been developed. Sperm chromosomes were obtained by using the fusion technique between zona-free hamster oocytes and human spermatozoa. Single- and double-colour chromosome painting was performed using DNA libraries specific for chromosomes X, Y and 21 on either unstained or G-banded preparations. The hybridization efficiency was very high (98%). The sequential staining technique is very useful for analyses of structural (stable) and numerical chromosome aberrations in human sperm and thus can increase the efficiency of the human sperm—hamster oocytes fusion system to assess the risk to human germ cells as a result of endogenous and exogenous factors.
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Colls, P., Templado, C., Martinez-Pasarell, O. et al. Sequential G-banding FISH on human sperm chromosomes. Chromosome Res 5, 457–461 (1997). https://doi.org/10.1023/A:1018464929628
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DOI: https://doi.org/10.1023/A:1018464929628