Abstract
Escherichia coli JM109(DE3) harboring expression plasmid pkAQNÆC30, which carries the Thermus protease aqualysin I (AQI) gene, was cultivated with glucose as a sole carbon source. The final cell concentration was over 15 g dry weight/l and the amount of AQI produced reached approximately 130 kU/ml broth. Moreover, by using two carbon sources, glucose and glycerol, the production yield was increased to over 200 kU AQI/ml, while suppressing the formation of inhibitory acetic acid.
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Tsuji, K., Iijima, M., Matsuzawa, H. et al. Over-production of Thermus protease in dense culture of Escherichia coli using two carbon sources. Biotechnology Techniques 11, 395–398 (1997). https://doi.org/10.1023/A:1018460521315
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DOI: https://doi.org/10.1023/A:1018460521315