Abstract
As astaxanthin could not be extracted with various organic solvents from intact thick-walled cyst cells of Haematococcus pluvialis, cells were held in 40% (v/v) acetone for 2 min at 80°C, followed by lyophilization or treatment of cells with specific lytic enzymes. By these treatments, 70% of the astaxanthin was extracted from the cells. Furthermore, the heat-acetone treatment was useful to remove chlorophyll specifically from the cells, contrary to saponification.
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References
Benemann, JR (1992) J Appl Phycol 4:233–245
Borowitzka, MA, Huisman, JM and Osborn, A (1991) J Appl Phycol 3:295–304
Boussiba, S and Vonshak, A (1991) Plant Cell Physiol 32:1077–1082
Britton, G (1985) Methods Enzymol 111:113–149
Cheeke, PR (1997) Natural Toxicants in Feeds, Forages and Poisonous Plants, Danville: Interstate Publ.
Cordero, B, Otero, A, Patino, M, Arredondo, BO and Fabregas, J (1996) Biotechnol Lett 18:213–218
Grung, M, D'Souza, FML, Borowitzka, M and Liaaen-Jensen, S (1992) J Appl Phycol 4:165–171
Harker, M, Tsavalos, AJ and Young, AJ (1996) J Ferment Bioeng 82:113–118
Kobayashi, M, Kakizono, T and Nagai, S (1991) J Ferment Bioeng 71:335–339
Kobayashi, M, Kakizono, T and Nagai, S (1993) Appl Environ Microbiol 59:867–873
Kobayashi, M, Kurimura, Y, Kakizono, T, Nishio, N and Tsuji, Y (1997a) J Ferment Bioeng 84:49–52
Kobayashi, M, Kurimura, Y and Tsuji, Y (1997b) Biotechnol Lett 19:507–509
Kobayashi, M, Hirai, N, Kurimura, Y, Ohigashi, H and Tsuji, Y (1997c) Plant Growth Regul (in press)
Kobayashi, M, Kakizono, T, Nishio, N, Nagai, S, Kurimura, Y and Tsuji, Y (1997d) Appl Microbiol Biotechnol (in press)
Lee, YK and Soh, CW (1991) J Phycol 27:575–577
Miki, W (1991) Pure Appl Chem 63:141–146
Okagbue, RN and Lewis, MJ (1983) Biotechnol Lett 5:731–736
Renstrøm, B, Borch, G, Skulberg, OM and Liaaen-Jensen, S (1981) Phytochemistry 20:2561–2564
Sommer, TR, Potts, WT and Morrissy NM (1991) Aquaculture 94:79–88
Tjahjono, AE, Hayama, Y, Kakizono, T, Terada, Y, Nishio, N and Nagai, S (1994) Biotechnol Lett 16:133–138
Yuan, JP, Gong, XD and Chen, F (1996) Biotechnol Tech 10:655–660
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Kobayashi, M., Kurimura, Y., Sakamoto, Y. et al. Selective extraction of astaxanthin and chlorophyll from the green alga Haematococcus pluvialis. Biotechnology Techniques 11, 657–660 (1997). https://doi.org/10.1023/A:1018455209445
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DOI: https://doi.org/10.1023/A:1018455209445