Biotechnology Techniques

, Volume 11, Issue 9, pp 657–660 | Cite as

Selective extraction of astaxanthin and chlorophyll from the green alga Haematococcus pluvialis

  • M. Kobayashi
  • Y. Kurimura
  • Y. Sakamoto
  • Y. Tsuji


As astaxanthin could not be extracted with various organic solvents from intact thick-walled cyst cells of Haematococcus pluvialis, cells were held in 40% (v/v) acetone for 2 min at 80°C, followed by lyophilization or treatment of cells with specific lytic enzymes. By these treatments, 70% of the astaxanthin was extracted from the cells. Furthermore, the heat-acetone treatment was useful to remove chlorophyll specifically from the cells, contrary to saponification.


Enzyme Acetone Chlorophyll Organic Solvent Green Alga 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Chapman and Hall 1997

Authors and Affiliations

  • M. Kobayashi
    • 1
  • Y. Kurimura
    • 1
  • Y. Sakamoto
    • 1
  • Y. Tsuji
    • 1
  1. 1.Research Laboratory of Higashimaru Shoyu Co., Ltd.Tatsuno, HyogoJapan

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