Abstract
Hairy roots of strawberry were cultivated in three different types of bioreactors: an air-sparged bioreactor (control), a droplet bioreactor and a mist bioreactor. The highest biomass yields (3.7 g dry wt/l) were achieved in the air-sparged and in the mist bioreactor. In the droplet bioreactor the cultivation medium was insufficiently atomized into droplets and nutrient uptake and growth were slower due to uneven wetting of hairy roots.
Similar content being viewed by others
References
Buitelaar, R.M., Lanfenhoff, A.A.M., Heidstra, R. and Tramper, J. (1991) Enzyme Microb. Technol. 31, 487–494.
Davioud, E., Kan, C., Hamon, J. Tempe, J. and Husson, H.-P. (1989) Phytochem. 28, 2675–2680.
DiIorio, A.A., Cheetham, R.D. and Weathers, P.J. (1992) Appl. Microb. Biotechnol. 37, 457–462.
Gamborg, O.L., Miller, R.A. and Ojima, K. (1968) Exp. Cell Res. 50, 151–158.
Gomori, G.A (1942) J. Lab. Clin. Med. 27, 955–960.
Kim, Y.H. and Yoo, Y.J. (1993) Biotechnol. Techn. 7, 859–862.
Kondo, O., Honda, A., Taya, M. and Kobayashi, T. (1989) Appl. Microb. Technol. 32, 291–294.
Krombholz, R., Mersinger, R., Kreis, W. and Reinhard, E. (1992) Planta Med. 58, 328–33.
McKelvey, S., Gehrig, J., Hollar, K. and Curtis, W. (1993) Biotechnol. Techn. 8, 639–644.
Nuutila, A.M., Toivonen, L. and Kauppinen, V. (1994) Biotechnol. Techn. 7, 61–66.
Nuutila, A.M., Vestberg, M. and Kauppinen, V. (1995) Plant Cell Rep. 14, 505–509.
Sharp, J.M. and Doran, P.M. (1990) J. Biotechnol. 16, 171–186.
Whitney, P.J. (1992) Enzyme Microb. Technol. 14, 13–17.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nuutila, A., Lindqvist, AS. & Kauppinen, V. Growth of hairy root cultures of strawberry (Fragaria ¥ ananassa Duch.) in three different types of bioreactors. Biotechnology Techniques 11, 363–366 (1997). https://doi.org/10.1023/A:1018444117681
Issue Date:
DOI: https://doi.org/10.1023/A:1018444117681