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Adsorption of a Trichoderma reesei endoglucanase and cellobiohydrolase onto bleached Kraft fibres

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Abstract

Cellulases can be used to modify pulp fibres. For the development of biotechnical applications, a better understanding of the adsorption of cellulases onto commercial wood fibres is needed. In this work, the adsorption behaviour of purified CBH I and EG II on bleached Kraft fibres was investigated. Three variables were studied with respect to their effect on adsorption: fibre type (hardwood or softwood), fibre history (never-dried or once-dried), and ionic strength. The results showed that fibre history had the largest influence on the extent of adsorption of each enzyme. The effect of ionic strength was shown to be dependent on the enzyme and fibre type. At high ionic strength, CBH I exhibited a higher affinity for both once-dried and never-dried fibres at low enzyme concentrations; however, salt was shown to decrease the extent of adsorption at higher enzyme dosages. In contrast, salt increased the maximum adsorption of EG II, most notably on the once-dried hardwood fibres. Fibre type was also shown to affect adsorption behaviour. CBH I had a higher affinity for softwood fibres than for hardwood fibres at low enzyme concentrations. The maximum adsorption of EG II onto once-dried softwood fibres increased by 80% compared to the once-dried hardwood fibres. Interestingly, this did not correlate to in creased fibre hydrolysis.

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Authors and Affiliations

  1. Department of Wood and Paper Science, North Carolina State University, Box 8005, Raleigh, North Carolina, USA

    P. J. GERBER, T. W. JOYCE & J. A. HEITMANN

  2. VTT , Biotechnology and Food Research, PO Box 1501, FIN-02044 VTT, Finland

    M. SIIKA-AHO & J. BUCHERT

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  1. P. J. GERBER
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  2. T. W. JOYCE
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  3. J. A. HEITMANN
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  4. M. SIIKA-AHO
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  5. J. BUCHERT
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GERBER, P.J., JOYCE, T.W., HEITMANN, J.A. et al. Adsorption of a Trichoderma reesei endoglucanase and cellobiohydrolase onto bleached Kraft fibres. Cellulose 4, 255–268 (1997). https://doi.org/10.1023/A:1018444008305

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